Restriction map for
pFN21AE6247
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AocI Aor51HI ApaI ApoI AscI
AseI AsnI Asp718I AspI AvaI
AviII AvrII BalI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaJI BsaWI BseAI BsiEI BsiWI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HaeIII HapII HindIII HpaI
HpaII KpnI KspI MaeIII MamI
MfeI MluI MluNI MroI MscI
MspA1I MspI MunI MvnI NaeI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
StyI SwaI Tsp45I Tsp509I Tth111I
Van91I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AhdI 76 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI 308 AGCT AG^CT
Alw44I 88 GTGCAC G^TGCAC
ApaLI 88 GTGCAC G^TGCAC
Asp700I 304 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 76 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 88 GTGCAC G[AT]GC[AT]^C
BamHI 3 GGATCC G^GATCC
BanI 269 GGCGCC G^G[CT][GA]CC
BanII 172 GAGCCC G[GA]GC[CT]^C
BbeI 269 GGCGCC GGCGC^C
BfaI 142 CTAG C^TAG
BseRI 381 CTCCTC GAGGAG(10/8)
BsgI 622 GTGCAG GTGCAG(16/14)
BsiHKAI 88 GTGCAC G[AT]GC[AT]^C
BsmBI 67 GAGACG CGTCTC(1/5)
BsrDI 501 CATTGC GCAATG(2/0)
BstAPI 81 GCAGTCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraII 454 AGGACCC [GA]G^G[ACGT]CC[CT]
Eam1105I 76 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 304 GAAGAG CTCTTC(1/4)
EcoO109I 454 AGGACCC [GA]G^G[ACGT]CC[CT]
FauI 552 GCGGG CCCGC(4/6)
HaeII 269 GGCGCC [GA]GCGC^[CT]
HgaI 59 GACGC GACGC(5/10)
HincII 208 GTTGAC GT[CT]^[GA]AC
HindII 208 GTTGAC GT[CT]^[GA]AC
HphI 326 TCACC GGTGA(8/7)
KasI 269 GGCGCC G^GCGCC
Ksp632I 304 GAAGAG CTCTTC(1/4)
MaeI 142 CTAG C^TAG
MaeII 147 ACGT A^CGT
NarI 269 GGCGCC GG^CGCC
NspI 509 ACATGC [GA]CATG^[CT]
PpuMI 454 AGGACCC [GA]G^G[AT]CC[CT]
SapI 304 GAAGAGC GCTCTTC(1/4)
ScaI 569 AGTACT AGT^ACT
SfoI 269 GGCGCC GGC^GCC
SspI 436 AATATT AAT^ATT
TfiI 364 GAATC G^A[AT]TC
XbaI 141 TCTAGA T^CTAGA
XcmI 347 CCACCATACCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 304 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 36 enzymes) #
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AciI 537 GCGG CCGC(-3/-1)
552 GCGG
AcyI 59 GACGCC G[GA]^CG[CT]C
269 GGCGCC
AfaI 570 GTAC GT^AC
627 GTAC
AvaII 33 GGTCC G^G[AT]CC
455 GGACC
BbvI 285 GCAGC GCAGC(8/12)
591 GCTGC
BsaHI 59 GACGCC G[GA]^CG[CT]C
269 GGCGCC
BsmFI 57 GGGAC GGGAC(10/14)
478 GGGAC
BspMI 236 ACCTGC ACCTGC(4/8)
30 GCAGGT
BsrI 45 CCAGT ACTGG(1/-1)
213 CCAGT
BssKI 63 CCTGG ^CC[ACGT]GG
561 CCAGG
BssSI 37 CACGAG CACGAG(-5/-1)
616 CTCGTG
BstF5I 411 GGATG GGATG(2/0)
587 GGATG
BstNI 63 CCTGG CC^[AT]GG
561 CCAGG
BstXI 350 CCATACCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
506 CCAACATGCTGG
Cac8I 115 GCAAGC GC[ACGT]^[ACGT]GC
552 GCGGGC
CfoI 247 GCGC GCG^C
270 GCGC
Cfr13I 33 GGTCC G^G[ACGT]CC
455 GGACC
EcoRII 63 CCTGG ^CC[AT]GG
561 CCAGG
FokI 411 GGATG GGATG(9/13)
587 GGATG
HhaI 247 GCGC GCG^C
270 GCGC
Hin1I 59 GACGCC G[GA]^CG[CT]C
269 GGCGCC
HinP1I 247 GCGC G^CGC
270 GCGC
MseI 217 TTAA T^TAA
377 TTAA
MslI 351 CATACCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
528 CACAGTGATG
MvaI 63 CCTGG CC^[AT]GG
561 CCAGG
PleI 337 GACTC GAGTC(4/5)
480 GACTC
PstI 238 CTGCAG CTGCA^G
370 CTGCAG
RsaI 570 GTAC GT^AC
627 GTAC
Sau96I 33 GGTCC G^G[ACGT]CC
455 GGACC
ScrFI 63 CCTGG CC^[ACGT]GG
561 CCAGG
SfcI 238 CTGCAG C^T[GA][CT]AG
370 CTGCAG
TaqI 318 TCGA T^CGA
545 TCGA
Tru9I 217 TTAA T^TAA
377 TTAA
TseI 285 GCAGC G^C[AT]GC
591 GCTGC
TspRI 368 CACTG CA[GC]TG[ACGT][ACGT]^
530 CAGTG
TthHB8I 318 TCGA T^CGA
545 TCGA
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# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AlwI AlwNI BmyI BpmI BsiYI
BslI BsmAI Bsp1286I BstYI DdeI
DpnII Fnu4HI HinfI ItaI MboI
MboII MflI MnlI MwoI NdeII
NlaIII NlaIV Sau3AI SfaNI XhoII