Restriction map for
pFN21AE1558
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# NO CUT ENZYMES ( 139 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AvaII AviII
AvrII BalI BbrPI BcgI BciVI
BcnI BfrI BglII BlnI BsaAI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I Cfr10I Cfr13I
ClaI CpoI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FseI FspI HgaI HincII
HindII HindIII HpaI KpnI Ksp632I
KspI MaeII MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PflMI PinAI PmaCI
PmeI PmlI PpuMI Psp1406I PvuI
RsrII SacII SalI SapI Sau96I
SbfI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Van91I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AcsI 770 AAATTC [GA]^AATT[CT]
AcyI 358 GGCGCC G[GA]^CG[CT]C
AlwNI 459 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 770 AAATTC [GA]^AATT[CT]
AseI 1018 ATTAAT AT^TAAT
AsnI 1018 ATTAAT AT^TAAT
Asp700I 914 GAATCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 183 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 98 CTCGAG C^[CT]CG[GA]G
BamHI 1004 GGATCC G^GATCC
BanI 358 GGCGCC G^G[CT][GA]CC
BbeI 358 GGCGCC GGCGC^C
BbvCI 985 CCTCAGC CCTCAGC(-5/-2)
BclI 1029 TGATCA T^GATCA
BfaI 857 CTAG C^TAG
BglI 283 GCCATCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 388 GCTCAGC GC^T[ACGT]AGC
BpmI 156 CTGGAG CTGGAG(16/14)
Bpu1102I 388 GCTCAGC GC^T[ACGT]AGC
BsaHI 358 GGCGCC G[GA]^CG[CT]C
BsaI 124 GAGACC GGTCTC(1/5)
BsgI 14 CTGCAC GTGCAG(16/14)
BsmBI 239 CGTCTC CGTCTC(1/5)
BsmFI 31 GTCCC GGGAC(10/14)
BsoBI 98 CTCGAG C^[CT]CG[GA]G
BspHI 651 TCATGA T^CATGA
BspMI 18 ACCTGC ACCTGC(4/8)
BsrDI 1033 CATTGC GCAATG(2/0)
BstAPI 292 GCATTTATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 533 CCAAGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1004 GGATCC [GA]^GATC[CT]
CelII 388 GCTCAGC GC^T[ACGT]AGC
DraI 942 TTTAAA TTT^AAA
FauI 105 GCGGG CCCGC(4/6)
FbaI 1029 TGATCA T^GATCA
HaeII 358 GGCGCC [GA]GCGC^[CT]
HaeIII 607 GGCC GG^CC
HapII 63 CCGG C^CGG
Hin1I 358 GGCGCC G[GA]^CG[CT]C
HpaII 63 CCGG C^CGG
KasI 358 GGCGCC G^GCGCC
MaeI 857 CTAG C^TAG
MflI 1004 GGATCC [GA]^GATC[CT]
MspI 63 CCGG C^CGG
NarI 358 GGCGCC GG^CGCC
PaeR7I 98 CTCGAG C^TCGAG
PflFI 183 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 210 GACCATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1018 ATTAAT AT^TAAT
RcaI 651 TCATGA T^CATGA
ScaI 402 AGTACT AGT^ACT
SfoI 358 GGCGCC GGC^GCC
SmlI 98 CTCGAG C^T[CT][GA]AG
Tth111I 183 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 991 CCAGAAGATAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 98 CTCGAG C^TCGAG
XhoII 1004 GGATCC [GA]^GATC[CT]
XmnI 914 GAATCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
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AspHI 715 GAGCTC G[AT]GC[AT]^C
1054 GAGCTC
BbsI 161 GAAGAC GAAGAC(2/6)
217 GTCTTC
BpuAI 161 GAAGAC GAAGAC(2/6)
217 GTCTTC
BsiHKAI 715 GAGCTC G[AT]GC[AT]^C
1054 GAGCTC
BsmAI 124 GAGAC GTCTC(1/5)
240 GTCTC
HphI 335 TCACC GGTGA(8/7)
900 TCACC
MspA1I 176 CAGCTG C[AC]G^C[GT]G
817 CAGCTG
NlaIV 358 GGCGCC GG[ACGT]^[ACGT]CC
1004 GGATCC
PleI 164 GACTC GAGTC(4/5)
204 GACTC
PstI 20 CTGCAG CTGCA^G
512 CTGCAG
PvuII 176 CAGCTG CAG^CTG
817 CAGCTG
SacI 715 GAGCTC GAGCT^C
1054 GAGCTC
SfcI 20 CTGCAG C^T[GA][CT]AG
512 CTGCAG
TaqI 99 TCGA T^CGA
144 TCGA
TfiI 914 GAATC G^A[AT]TC
1046 GAATC
TthHB8I 99 TCGA T^CGA
144 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AfaI AluI AlwI BanII
BbvI BmyI BsaJI BseRI BsiYI
BslI Bsp1286I BssKI BstF5I BstNI
Cac8I CfoI DdeI DpnII EcoRII
Fnu4HI FokI HhaI HinP1I HinfI
ItaI MaeIII MboI MboII MnlI
MseI MslI MvaI MwoI NdeII
NlaIII RsaI Sau3AI ScrFI SfaNI
Tru9I TseI Tsp45I Tsp509I TspRI