Restriction map for
pFN21AE6145
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# NO CUT ENZYMES ( 197 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BciVI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmAI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DdeI DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaI HpaII
KasI KpnI Ksp632I KspI MaeI
MaeII MaeIII MboII MfeI MluI
MluNI MroI MscI MseI MslI
MspA1I MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIII NotI NruI NsiI
NspI PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
TfiI Tru9I Tsp45I Tsp509I TspRI
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AlwI 66 GGATC GGATC(4/5)
AspHI 227 GAGCTC G[AT]GC[AT]^C
AvaII 6 GGACC G^G[AT]CC
BanII 227 GAGCTC G[GA]GC[CT]^C
BcgI 137 GCAAAATGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BmyI 227 GAGCTC G[AGT]GC[ACT]^C
BsaBI 48 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 87 CCTGGG C^C[ACGT][ACGT]GG
BsiHKAI 227 GAGCTC G[AT]GC[AT]^C
BsiYI 57 CCCAGAACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 57 CCCAGAACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 5 GGGAC GGGAC(10/14)
Bsp1286I 227 GAGCTC G[AGT]GC[ACT]^C
BsrDI 234 CATTGC GCAATG(2/0)
BstBI 253 TTCGAA TT^CGAA
BstYI 66 GGATCT [GA]^GATC[CT]
Cfr13I 6 GGACC G^G[ACGT]CC
DraII 5 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO109I 5 GGGACCC [GA]G^G[ACGT]CC[CT]
HphI 187 GGTGA GGTGA(8/7)
MamI 48 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 66 GGATCT [GA]^GATC[CT]
NspV 253 TTCGAA TT^CGAA
PpuMI 5 GGGACCC [GA]G^G[AT]CC[CT]
PstI 158 CTGCAG CTGCA^G
SacI 227 GAGCTC GAGCT^C
Sau96I 6 GGACC G^G[ACGT]CC
SfcI 158 CTGCAG C^T[GA][CT]AG
SfuI 253 TTCGAA TT^CGAA
SmlI 199 CTTGAG C^T[CT][GA]AG
StuI 84 AGGCCT AGG^CCT
TaqI 254 TCGA T^CGA
TthHB8I 254 TCGA T^CGA
XcmI 53 CCATCCCAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 66 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 24 GTAC GT^AC
204 GTAC
AluI 77 AGCT AG^CT
228 AGCT
BbvI 128 GCAGC GCAGC(8/12)
160 GCAGC
BpmI 42 CTGGAG CTGGAG(16/14)
222 CTGGAG
BssKI 87 CCTGG ^CC[ACGT]GG
168 CCAGG
BstNI 87 CCTGG CC^[AT]GG
168 CCAGG
Cac8I 150 GCAAGC GC[ACGT]^[ACGT]GC
238 GCCAGC
DpnII 67 GATC ^GATC
144 GATC
EcoRII 87 CCTGG ^CC[AT]GG
168 CCAGG
Fnu4HI 128 GCAGC GC^[ACGT]GC
160 GCAGC
HaeIII 85 GGCC GG^CC
193 GGCC
ItaI 128 GCAGC GC^[ACGT]GC
160 GCAGC
MboI 67 GATC ^GATC
144 GATC
MvaI 87 CCTGG CC^[AT]GG
168 CCAGG
NdeII 67 GATC ^GATC
144 GATC
NlaIV 5 GGGACC GG[ACGT]^[ACGT]CC
6 GGACCC
RsaI 24 GTAC GT^AC
204 GTAC
Sau3AI 67 GATC ^GATC
144 GATC
ScrFI 87 CCTGG CC^[ACGT]GG
168 CCAGG
SfaNI 48 GATGC GCATC(5/9)
242 GCATC
TseI 128 GCAGC G^C[AT]GC
160 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 5 enzymes) #
#########################################
BsrI BstF5I FokI MnlI MwoI