Restriction map for
pFN21AE4370
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BamHI BbeI
BbrPI BciVI BcnI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BseAI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI
BstEII BstPI BstXI Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I FseI FspI
HgaI Hin1I HincII HindII HindIII
HinfI HpaI KasI KpnI KspI
MaeI MamI MfeI MluI MroI
MunI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspBI
SspI StyI SwaI TaqI TfiI
Tth111I TthHB8I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccI 204 GTATAC GT^[AC][GT]AC
AccII 389 CGCG CG^CG
AcsI 912 GAATTT [GA]^AATT[CT]
AlwNI 356 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 668 GGGCCC GGGCC^C
ApoI 912 GAATTT [GA]^AATT[CT]
AvaII 852 GGACC G^G[AT]CC
BanI 811 GGCACC G^G[CT][GA]CC
BbsI 877 GAAGAC GAAGAC(2/6)
BglI 119 GCCTGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 877 GAAGAC GAAGAC(2/6)
BsaWI 957 TCCGGT [AT]^CCGG[AT]
BseRI 662 GAGGAG GAGGAG(10/8)
BsmAI 738 GAGAC GTCTC(1/5)
BsmI 1133 GAATGC GAATGC(1/-1)
BspMI 379 ACCTGC ACCTGC(4/8)
BsrDI 460 GCAATG GCAATG(2/0)
Bst1107I 204 GTATAC GTA^TAC
BstUI 389 CGCG CG^CG
BstZ17I 204 GTATAC GTA^TAC
EcoT22I 561 ATGCAT ATGCA^T
HaeII 1027 GGCGCT [GA]GCGC^[CT]
MaeII 604 ACGT A^CGT
MseI 764 TTAA T^TAA
MslI 564 CATGGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 389 CGCG CG^CG
NsiI 561 ATGCAT ATGCA^T
NspI 978 GCATGC [GA]CATG^[CT]
PflMI 520 CCAACTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 466 CTGCAG CTGCA^G
PvuII 97 CAGCTG CAG^CTG
ScaI 51 AGTACT AGT^ACT
SfcI 466 CTGCAG C^T[GA][CT]AG
SphI 978 GCATGC GCATG^C
StuI 126 AGGCCT AGG^CCT
Tru9I 764 TTAA T^TAA
Van91I 520 CCAACTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 629 CCAAAGGGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 52 GTAC GT^AC
871 GTAC
BbvCI 361 GCTGAGG CCTCAGC(-5/-2)
865 GCTGAGG
BcgI 373 CGAAGAACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
419 CGAAGCCAGTGC
BclI 491 TGATCA T^GATCA
926 TGATCA
BsaJI 354 CCCAGG C^C[ACGT][ACGT]GG
1018 CCAGGG
BsgI 134 GTGCAG GTGCAG(16/14)
427 GTGCAG
BstYI 29 GGATCT [GA]^GATC[CT]
1040 GGATCT
CfoI 390 GCGC GCG^C
1028 GCGC
DraII 667 GGGGCCC [GA]G^G[ACGT]CC[CT]
668 GGGCCCC
EcoO109I 667 GGGGCCC [GA]G^G[ACGT]CC[CT]
668 GGGCCCC
FbaI 491 TGATCA T^GATCA
926 TGATCA
HapII 332 CCGG C^CGG
958 CCGG
HhaI 390 GCGC GCG^C
1028 GCGC
HinP1I 390 GCGC G^CGC
1028 GCGC
HpaII 332 CCGG C^CGG
958 CCGG
MflI 29 GGATCT [GA]^GATC[CT]
1040 GGATCT
MspI 332 CCGG C^CGG
958 CCGG
RsaI 52 GTAC GT^AC
871 GTAC
SacI 87 GAGCTC GAGCT^C
1124 GAGCTC
SapI 84 GAAGAGC GCTCTTC(1/4)
993 GAAGAGC
SfaNI 439 GATGC GCATC(5/9)
688 GCATC
TspRI 425 CAGTG CA[GC]TG[ACGT][ACGT]^
816 CAGTG
XhoII 29 GGATCT [GA]^GATC[CT]
1040 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AluI AlwI AspHI BalI
BanII BbvI BmyI BpmI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EaeI EarI EcoRII
FauI Fnu4HI FokI HaeIII HphI
ItaI Ksp632I MaeIII MboI MboII
MluNI MnlI MscI MspA1I MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI TseI Tsp45I Tsp509I