Restriction map for
pFN21AE6316
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
AlwNI AocI Aor51HI ApaI AscI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HindIII HpaI HpaII KasI
KpnI KspI MaeII MamI MfeI
MluI MluNI MroI MscI MspI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SwaI Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 26 enzymes) #
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AflIII 125 ACATGT A^C[GA][CT]GT
Alw44I 29 GTGCAC G^TGCAC
ApaLI 29 GTGCAC G^TGCAC
BanI 399 GGCACC G^G[CT][GA]CC
BbsI 589 GAAGAC GAAGAC(2/6)
BglI 559 GCCTCCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 461 CTCCAG CTGGAG(16/14)
BpuAI 589 GAAGAC GAAGAC(2/6)
BsmFI 775 GGGAC GGGAC(10/14)
BsmI 794 GCATTC GAATGC(1/-1)
BspLU11I 125 ACATGT A^CATGT
BspMI 593 ACCTGC ACCTGC(4/8)
BstF5I 661 CATCC GGATG(2/0)
BstXI 860 CCAATGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 401 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 1072 CCTGGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 160 GAATTC G^AATTC
FauI 599 CCCGC CCCGC(4/6)
FokI 661 CATCC GGATG(9/13)
MslI 580 CATAACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 224 CCATGG C^CATGG
PflMI 219 CCACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 215 GAGTC GAGTC(4/5)
SexAI 402 ACCTGGT A^CC[AT]GGT
SfaNI 100 GCATC GCATC(5/9)
Van91I 219 CCACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AciI 435 CCGC CCGC(-3/-1)
600 CCGC
AcsI 160 GAATTC [GA]^AATT[CT]
523 GAATTT
AfaI 712 GTAC GT^AC
763 GTAC
ApoI 160 GAATTC [GA]^AATT[CT]
523 GAATTT
AseI 769 ATTAAT AT^TAAT
849 ATTAAT
AsnI 769 ATTAAT AT^TAAT
849 ATTAAT
BanII 446 GAGCTC G[GA]GC[CT]^C
880 GAGCTC
BfaI 541 CTAG C^TAG
877 CTAG
BsgI 842 CTGCAC GTGCAG(16/14)
1178 CTGCAC
BstYI 818 AGATCC [GA]^GATC[CT]
1202 AGATCC
DsaI 224 CCATGG C^C[GA][CT]GG
758 CCACGG
HincII 9 GTTGAC GT[CT]^[GA]AC
1247 GTCAAC
HindII 9 GTTGAC GT[CT]^[GA]AC
1247 GTCAAC
MaeI 541 CTAG C^TAG
877 CTAG
MflI 818 AGATCC [GA]^GATC[CT]
1202 AGATCC
MspA1I 1027 CAGCTG C[AC]G^C[GT]G
1175 CAGCTG
NspI 125 ACATGT [GA]CATG^[CT]
1188 ACATGC
PshBI 769 ATTAAT AT^TAAT
849 ATTAAT
PvuII 1027 CAGCTG CAG^CTG
1175 CAGCTG
RsaI 712 GTAC GT^AC
763 GTAC
SacI 446 GAGCTC GAGCT^C
880 GAGCTC
SfcI 62 CTACAG C^T[GA][CT]AG
147 CTATAG
SmlI 137 CTCAAG C^T[CT][GA]AG
905 CTCAAG
SspI 626 AATATT AAT^ATT
923 AATATT
StuI 66 AGGCCT AGG^CCT
295 AGGCCT
TaqI 426 TCGA T^CGA
441 TCGA
TfiI 72 GATTC G^A[AT]TC
180 GAATC
TthHB8I 426 TCGA T^CGA
441 TCGA
XcmI 371 CCAGCCTGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
857 CCACCAATGCCTTGG
XhoII 818 AGATCC [GA]^GATC[CT]
1202 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AluI AlwI AspHI AvaII BbvI
BmyI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstNI Cac8I Cfr13I DdeI
DpnII EarI EcoRII EcoT14I Fnu4HI
HaeIII HinfI HphI ItaI Ksp632I
MaeIII MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI StyI Tru9I
TseI Tsp45I Tsp509I TspRI