Restriction map for
pFN21AE6216
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# NO CUT ENZYMES ( 170 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BbvCI BciVI BfaI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRV EcoT14I EcoT22I
FauI FseI HaeII Hin1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MaeII MboII
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
TfiI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 51 CGCG CG^CG
AcsI 451 GAATTC [GA]^AATT[CT]
AflIII 347 ACATGT A^C[GA][CT]GT
AlwNI 4 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 451 GAATTC [GA]^AATT[CT]
AvaII 129 GGACC G^G[AT]CC
AviII 60 TGCGCA TGC^GCA
BcgI 548 GCATCAAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 140 TGATCA T^GATCA
BcnI 494 CCGGG CC^[GC]GG
BglI 477 GCCCAGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 282 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 233 CTCCTC GAGGAG(10/8)
BspLU11I 347 ACATGT A^CATGT
BstF5I 83 CATCC GGATG(2/0)
BstUI 51 CGCG CG^CG
BstYI 287 AGATCC [GA]^GATC[CT]
CfoI 61 GCGC GCG^C
DraII 128 AGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 166 GACCCCATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 9 CCTGATGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 128 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 451 GAATTC G^AATTC
FbaI 140 TGATCA T^GATCA
FokI 83 CATCC GGATG(9/13)
FspI 60 TGCGCA TGC^GCA
HapII 494 CCGG C^CGG
HhaI 61 GCGC GCG^C
HinP1I 61 GCGC G^CGC
HinfI 153 GACTC G^A[ACGT]TC
HpaII 494 CCGG C^CGG
MaeIII 190 GTGAC ^GT[ACGT]AC
MamI 282 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 287 AGATCC [GA]^GATC[CT]
MseI 352 TTAA T^TAA
MslI 328 CACTTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 312 CAGCTG C[AC]G^C[GT]G
MspI 494 CCGG C^CGG
MvnI 51 CGCG CG^CG
NciI 494 CCGGG CC^[GC]GG
NspI 347 ACATGT [GA]CATG^[CT]
PleI 153 GACTC GAGTC(4/5)
PpuMI 128 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 312 CAGCTG CAG^CTG
Tru9I 352 TTAA T^TAA
Tsp45I 190 GTGAC ^GT[GC]AC
XhoII 287 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AfaI 317 GTAC GT^AC
389 GTAC
AlwI 288 GATCC GGATC(4/5)
281 GGATC
BanII 367 GAGCCC G[GA]GC[CT]^C
379 GAGCCC
BmyI 367 GAGCCC G[AGT]GC[ACT]^C
379 GAGCCC
BpmI 157 CTGGAG CTGGAG(16/14)
469 CTGGAG
BsiYI 9 CCTGATGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
150 CCTGACTCTGG
BslI 9 CCTGATGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
150 CCTGACTCTGG
Bsp1286I 367 GAGCCC G[AGT]GC[ACT]^C
379 GAGCCC
Cfr13I 54 GGCCC G^G[ACGT]CC
129 GGACC
HaeIII 54 GGCC GG^CC
274 GGCC
HgaI 515 GCGTC GACGC(5/10)
537 GCGTC
HphI 138 GGTGA GGTGA(8/7)
488 TCACC
NlaIII 293 CATG CATG^
348 CATG
RsaI 317 GTAC GT^AC
389 GTAC
Sau96I 54 GGCCC G^G[ACGT]CC
129 GGACC
SfaNI 12 GATGC GCATC(5/9)
548 GCATC
Tsp509I 354 AATT ^AATT
452 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AciI AluI BbvI BsaJI BsmFI
BsrI BssKI BstNI Cac8I DdeI
DpnII EcoRII Fnu4HI ItaI MboI
MnlI MvaI MwoI NdeII NlaIV
Sau3AI ScrFI TseI TspRI