Restriction map for
pFN21AE4576
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# NO CUT ENZYMES ( 105 enzymes) #
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AatII AccI AccII AccIII AclI
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I AspEI AspI AviII BbrPI
BcgI BciVI BlpI Bpu1102I BsaAI
BsaWI BseAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BsrDI BsrGI
BssHII BssSI Bst1107I BstEII BstPI
BstUI BstZ17I Bsu36I CelII ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoT22I FseI FspI HgaI HpaI
KspI MaeII MfeI MluI MroI
MunI MvnI NaeI NdeI NgoMIV
NotI NruI NsiI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SfiI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 65 enzymes) #
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Acc65I 508 GGTACC G^GTACC
AcyI 2173 GGCGCC G[GA]^CG[CT]C
AfaI 509 GTAC GT^AC
AflII 1360 CTTAAG C^TTAAG
Asp718I 508 GGTACC G^GTACC
AspHI 1064 GAGCTC G[AT]GC[AT]^C
AvaI 62 CCCGAG C^[CT]CG[GA]G
AvrII 1881 CCTAGG C^CTAGG
BalI 2081 TGGCCA TGG^CCA
BanII 1064 GAGCTC G[GA]GC[CT]^C
BbeI 2173 GGCGCC GGCGC^C
BbvCI 27 CCTCAGC CCTCAGC(-5/-2)
BclI 246 TGATCA T^GATCA
BcnI 12 CCGGG CC^[GC]GG
BfrI 1360 CTTAAG C^TTAAG
BglI 7 GCCTGCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1881 CCTAGG C^CTAGG
BmyI 1064 GAGCTC G[AGT]GC[ACT]^C
BsaBI 18 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2173 GGCGCC G[GA]^CG[CT]C
BsaI 1585 GGTCTC GGTCTC(1/5)
BseRI 440 GAGGAG GAGGAG(10/8)
BsiEI 1538 CGACCG CG[GA][CT]^CG
BsiHKAI 1064 GAGCTC G[AT]GC[AT]^C
BsmI 617 GCATTC GAATGC(1/-1)
BsoBI 62 CCCGAG C^[CT]CG[GA]G
Bsp1286I 1064 GAGCTC G[AGT]GC[ACT]^C
BstAPI 1365 GCACAGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 155 TTCGAA TT^CGAA
DraIII 1366 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 2081 TGGCCA [CT]^GGCC[GA]
EarI 1929 GAAGAG CTCTTC(1/4)
EcoNI 2064 CCTGCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1944 GAATTC G^AATTC
EcoRV 307 GATATC GAT^ATC
FauI 2130 CCCGC CCCGC(4/6)
FbaI 246 TGATCA T^GATCA
HaeII 2173 GGCGCC [GA]GCGC^[CT]
Hin1I 2173 GGCGCC G[GA]^CG[CT]C
HindIII 1728 AAGCTT A^AGCTT
KasI 2173 GGCGCC G^GCGCC
KpnI 508 GGTACC GGTAC^C
Ksp632I 1929 GAAGAG CTCTTC(1/4)
MamI 18 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2081 TGGCCA TGG^CCA
MscI 2081 TGGCCA TGG^CCA
NarI 2173 GGCGCC GG^CGCC
NciI 12 CCGGG CC^[GC]GG
NcoI 1029 CCATGG C^CATGG
NheI 2247 GCTAGC G^CTAGC
NspV 155 TTCGAA TT^CGAA
PflMI 657 CCAACTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1351 GACCTTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 2090 CTGCAG CTGCA^G
RsaI 509 GTAC GT^AC
SacI 1064 GAGCTC GAGCT^C
SexAI 1642 ACCAGGT A^CC[AT]GGT
SfoI 2173 GGCGCC GGC^GCC
SfuI 155 TTCGAA TT^CGAA
SphI 1487 GCATGC GCATG^C
TaqI 156 TCGA T^CGA
Tsp45I 2032 GTGAC ^GT[GC]AC
TthHB8I 156 TCGA T^CGA
Van91I 657 CCAACTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 117 CCATATTAACTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AflIII 314 ACATGT A^C[GA][CT]GT
1791 ACATGT
BamHI 50 GGATCC G^GATCC
427 GGATCC
BbsI 2011 GAAGAC GAAGAC(2/6)
93 GTCTTC
BglII 764 AGATCT A^GATCT
1803 AGATCT
BpuAI 2011 GAAGAC GAAGAC(2/6)
93 GTCTTC
BspLU11I 314 ACATGT A^CATGT
1791 ACATGT
BspMI 2088 ACCTGC ACCTGC(4/8)
298 GCAGGT
BsrBI 57 CCGCTC CCGCTC(-3/-3)
1825 GAGCGG
BsrFI 952 GCCGGT [GA]^CCGG[CT]
2192 ACCGGC
BstXI 566 CCAAAATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1754 CCACACTGGTGG
Cfr10I 952 GCCGGT [GA]^CCGG[CT]
2192 ACCGGC
HincII 652 GTTGAC GT[CT]^[GA]AC
1696 GTTGAC
HindII 652 GTTGAC GT[CT]^[GA]AC
1696 GTTGAC
MslI 1605 CATCATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1755 CACACTGGTG
PpuMI 1037 AGGTCCT [GA]G^G[AT]CC[CT]
1349 AGGACCT
SfcI 1574 CTGTAG C^T[GA][CT]AG
2090 CTGCAG
StuI 885 AGGCCT AGG^CCT
1010 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AcsI AluI AlwI AlwNI
ApoI AvaII BanI BbvI BfaI
BpmI BsaJI BsgI BsiYI BslI
BsmAI BsmFI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PvuII Sau3AI
Sau96I ScrFI SfaNI SmlI StyI
TfiI Tru9I TseI Tsp509I TspRI
XhoII