Restriction map for
pFN21AE1307
##################################
# NO CUT ENZYMES ( 111 enzymes) #
##################################
AccIII AclI AcsI AflII AflIII
AgeI Alw44I ApaLI ApoI AscI
AseI AsnI Asp700I AvrII BamHI
BbeI BbrPI BbsI BbvCI BciVI
BfrI BglI BglII BlnI BpuAI
BsaAI BsaBI BsaWI BseAI BsiWI
BsmI BspDI BspEI BspLU11I BsrBI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DrdI DsaI EagI EclXI Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FseI HindIII HinfI HpaI KasI
KspI MaeIII MamI MfeI MluI
MroI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspI
SwaI TfiI Tsp45I XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AatII 460 GACGTC GACGT^C
Acc65I 572 GGTACC G^GTACC
AccII 269 CGCG CG^CG
AcyI 460 GACGTC G[GA]^CG[CT]C
AocI 993 CCTGAGG CC^T[ACGT]AGG
Aor51HI 824 AGCGCT AGC^GCT
ApaI 341 GGGCCC GGGCC^C
Asp718I 572 GGTACC G^GTACC
AspHI 1372 GAGCTC G[AT]GC[AT]^C
AspI 720 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 338 CCCGGG C^[CT]CG[GA]G
AviII 802 TGCGCA TGC^GCA
BcgI 242 GCAGCTGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1126 CTAG C^TAG
BlpI 1173 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1173 GCTCAGC GC^T[ACGT]AGC
BsaHI 460 GACGTC G[GA]^CG[CT]C
BseRI 300 CTCCTC GAGGAG(10/8)
BsiEI 954 CGACCG CG[GA][CT]^CG
BsiHKAI 1372 GAGCTC G[AT]GC[AT]^C
BsoBI 338 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1214 TGTACA T^GTACA
BspHI 593 TCATGA T^CATGA
BsrDI 470 GCAATG GCAATG(2/0)
BsrFI 182 ACCGGC [GA]^CCGG[CT]
BsrGI 1214 TGTACA T^GTACA
BstAPI 239 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 269 CGCG CG^CG
BstYI 755 AGATCC [GA]^GATC[CT]
Bsu36I 993 CCTGAGG CC^T[ACGT]AGG
CelII 1173 GCTCAGC GC^T[ACGT]AGC
Cfr10I 182 ACCGGC [GA]^CCGG[CT]
DraIII 603 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 824 AGCGCT AGC^GCT
Eco81I 993 CCTGAGG CC^T[ACGT]AGG
FspI 802 TGCGCA TGC^GCA
HgaI 778 GACGC GACGC(5/10)
Hin1I 460 GACGTC G[GA]^CG[CT]C
HincII 145 GTTGAC GT[CT]^[GA]AC
HindII 145 GTTGAC GT[CT]^[GA]AC
KpnI 572 GGTACC GGTAC^C
MaeI 1126 CTAG C^TAG
MflI 755 AGATCC [GA]^GATC[CT]
MseI 1067 TTAA T^TAA
MvnI 269 CGCG CG^CG
PflFI 720 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 417 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 243 CAGCTG CAG^CTG
RcaI 593 TCATGA T^CATGA
SacI 1372 GAGCTC GAGCT^C
SapI 1044 GCTCTTC GCTCTTC(1/4)
SmaI 338 CCCGGG CCC^GGG
SspBI 1214 TGTACA T^GTACA
StuI 921 AGGCCT AGG^CCT
Tru9I 1067 TTAA T^TAA
Tsp509I 19 AATT ^AATT
Tth111I 720 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 417 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 755 AGATCC [GA]^GATC[CT]
XmaCI 338 CCCGGG C^CCGGG
XmaI 338 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 79 GTCTAC GT^[AC][GT]AC
1285 GTAGAC
AhdI 148 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
584 GACACAATGTC
AspEI 148 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
584 GACACAATGTC
BalI 131 TGGCCA TGG^CCA
425 TGGCCA
BanI 572 GGTACC G^G[CT][GA]CC
876 GGTGCC
BsaI 534 GAGACC GGTCTC(1/5)
1023 GAGACC
BsiYI 42 CCCTCTGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
417 CCACTGCCTGG
BslI 42 CCCTCTGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
417 CCACTGCCTGG
BsmBI 458 GAGACG CGTCTC(1/5)
505 GAGACG
BstXI 38 CCACCCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1319 CCAACTTCCTGG
Cac8I 427 GCCAGC GC[ACGT]^[ACGT]GC
899 GCTGGC
EaeI 131 TGGCCA [CT]^GGCC[GA]
425 TGGCCA
Eam1105I 148 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
584 GACACAATGTC
EarI 1045 CTCTTC CTCTTC(1/4)
70 GAAGAG
Ksp632I 1045 CTCTTC CTCTTC(1/4)
70 GAAGAG
MboII 70 GAAGA GAAGA(8/7)
1046 TCTTC
MluNI 131 TGGCCA TGG^CCA
425 TGGCCA
MscI 131 TGGCCA TGG^CCA
425 TGGCCA
MslI 309 CATCTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
679 CACTTATATG
PpuMI 60 AGGACCC [GA]G^G[AT]CC[CT]
989 GGGACCT
PstI 237 CTGCAG CTGCA^G
1273 CTGCAG
SfcI 237 CTGCAG C^T[GA][CT]AG
1273 CTGCAG
TaqI 512 TCGA T^CGA
582 TCGA
TthHB8I 512 TCGA T^CGA
582 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BanII BbvI BclI BcnI
BmyI BpmI BsaJI BsgI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI CfoI Cfr13I DdeI
DpnII DraII EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MaeII MboI MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TseI
TspRI XcmI