Restriction map for
pFN21AB5757
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# NO CUT ENZYMES ( 94 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI Alw44I ApaLI
ApoI AscI AseI AsnI Asp718I
AviII AvrII BcgI BclI BfrI
BglII BlnI BsaBI BsaI BsaWI
BseAI BsiWI BsmI BspDI BspEI
BspHI BsrDI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DraIII DrdI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KpnI KspI MamI MfeI
MluI MroI MseI MunI NaeI
NcoI NdeI NgoMIV NheI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
ScaI SfuI SgrAI SnaBI SpeI
SphI SspI SwaI TfiI Tru9I
Tsp509I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AhdI 1640 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1056 CCTTAGG CC^T[ACGT]AGG
Asp700I 306 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1640 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 1303 CACGTG CAC^GTG
BciVI 160 GTATCC GTATCC(6/5)
BfaI 1150 CTAG C^TAG
BsaAI 1303 CACGTG [CT]AC^GT[GA]
Bsp1407I 674 TGTACA T^GTACA
BsrBI 1066 GAGCGG CCGCTC(-3/-3)
BsrGI 674 TGTACA T^GTACA
BssHII 1624 GCGCGC G^CGCGC
BssSI 1867 CTCGTG CACGAG(-5/-1)
BstAPI 1466 GCAGCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1056 CCTTAGG CC^T[ACGT]AGG
Eam1105I 1640 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 601 CTCTTC CTCTTC(1/4)
Eco81I 1056 CCTTAGG CC^T[ACGT]AGG
HincII 1609 GTCAAC GT[CT]^[GA]AC
HindII 1609 GTCAAC GT[CT]^[GA]AC
HinfI 1687 GACTC G^A[ACGT]TC
Ksp632I 601 CTCTTC CTCTTC(1/4)
MaeI 1150 CTAG C^TAG
NotI 1632 GCGGCCGC GC^GGCCGC
PleI 1687 GACTC GAGTC(4/5)
PmaCI 1303 CACGTG CAC^GTG
PmlI 1303 CACGTG CAC^GTG
SapI 600 GCTCTTC GCTCTTC(1/4)
SexAI 1643 ACCAGGT A^CC[AT]GGT
SfiI 804 GGCCATAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 1330 CTCAAG C^T[CT][GA]AG
SspBI 674 TGTACA T^GTACA
XmnI 306 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AflIII 701 ACATGT A^C[GA][CT]GT
2333 ACATGT
ApaI 1541 GGGCCC GGGCC^C
2308 GGGCCC
AspI 1753 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1945 GACAATGTC
BamHI 385 GGATCC G^GATCC
1289 GGATCC
BsiEI 1584 CGGCCG CG[GA][CT]^CG
1633 CGGCCG
BspLU11I 701 ACATGT A^CATGT
2333 ACATGT
BsrFI 443 ACCGGC [GA]^CCGG[CT]
1396 ACCGGC
Cfr10I 443 ACCGGC [GA]^CCGG[CT]
1396 ACCGGC
EagI 1584 CGGCCG C^GGCCG
1633 CGGCCG
EclXI 1584 CGGCCG C^GGCCG
1633 CGGCCG
Eco52I 1584 CGGCCG C^GGCCG
1633 CGGCCG
MslI 504 CACCTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1173 CACTCTGGTG
PflFI 1753 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1945 GACAATGTC
SbfI 456 CCTGCAGG CCTGCA^GG
687 CCTGCAGG
SmaI 272 CCCGGG CCC^GGG
1196 CCCGGG
Sse8387I 456 CCTGCAGG CCTGCA^GG
687 CCTGCAGG
Tth111I 1753 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1945 GACAATGTC
XcmI 1409 CCATCGAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2114 CCACCAGCCCCCTGG
XmaCI 272 CCCGGG C^CCGGG
1196 CCCGGG
XmaI 272 CCCGGG C^CCGGG
1196 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI Aor51HI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbsI BbvCI BbvI BcnI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI Eco47III
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
HphI ItaI KasI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV NspI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI StuI StyI
TaqI TseI Tsp45I TspRI TthHB8I
XhoII