Restriction map for
pFN21AE2306
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# NO CUT ENZYMES ( 127 enzymes) #
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AatII AccI AccII AccIII AclI
AflII AflIII AgeI Alw44I Aor51HI
ApaLI AscI AseI AsnI AvrII
BamHI BbeI BbsI BcgI BclI
BfrI BlnI BlpI Bpu1102I BpuAI
BsaWI BseAI BseRI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BsrI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EagI EarI EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HaeII HindIII
HinfI HpaI KasI Ksp632I KspI
MfeI MluI MroI MseI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PleI PmeI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Tru9I Van91I XbaI
XcmI XhoI
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# ONE CUT ENZYMES ( 66 enzymes) #
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Acc65I 620 GGTACC G^GTACC
AcyI 258 GACGCC G[GA]^CG[CT]C
AhdI 705 GACTGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 38 CCTCAGG CC^T[ACGT]AGG
ApaI 831 GGGCCC GGGCC^C
Asp700I 483 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 620 GGTACC G^GTACC
AspEI 705 GACTGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 402 GAGCAC G[AT]GC[AT]^C
AspI 688 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 347 TGCGCA TGC^GCA
BalI 104 TGGCCA TGG^CCA
BbrPI 222 CACGTG CAC^GTG
BbvCI 420 CCTCAGC CCTCAGC(-5/-2)
BbvI 468 GCTGC GCAGC(8/12)
BciVI 566 GTATCC GTATCC(6/5)
BfaI 679 CTAG C^TAG
BglII 807 AGATCT A^GATCT
BsaAI 222 CACGTG [CT]AC^GT[GA]
BsaBI 561 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 258 GACGCC G[GA]^CG[CT]C
BsaI 628 GAGACC GGTCTC(1/5)
BsiHKAI 402 GAGCAC G[AT]GC[AT]^C
BsmFI 91 GTCCC GGGAC(10/14)
BspMI 440 ACCTGC ACCTGC(4/8)
BssSI 868 CACGAG CACGAG(-5/-1)
Bsu36I 38 CCTCAGG CC^T[ACGT]AGG
CfoI 348 GCGC GCG^C
DsaI 459 CCATGG C^C[GA][CT]GG
Eam1105I 705 GACTGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 38 CCTCAGG CC^T[ACGT]AGG
EcoT14I 459 CCATGG C^C[AT][AT]GG
FauI 498 GCGGG CCCGC(4/6)
FspI 347 TGCGCA TGC^GCA
HgaI 258 GACGC GACGC(5/10)
HhaI 348 GCGC GCG^C
Hin1I 258 GACGCC G[GA]^CG[CT]C
HinP1I 348 GCGC G^CGC
HincII 733 GTCAAC GT[CT]^[GA]AC
HindII 733 GTCAAC GT[CT]^[GA]AC
HphI 702 GGTGA GGTGA(8/7)
KpnI 620 GGTACC GGTAC^C
MaeI 679 CTAG C^TAG
MaeIII 703 GTGAC ^GT[ACGT]AC
MamI 561 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 104 TGGCCA TGG^CCA
MscI 104 TGGCCA TGG^CCA
MslI 300 CATCATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 656 CAGCGG C[AC]G^C[GT]G
NcoI 459 CCATGG C^CATGG
NlaIII 460 CATG CATG^
PflFI 688 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 222 CACGTG CAC^GTG
PmlI 222 CACGTG CAC^GTG
PstI 469 CTGCAG CTGCA^G
SfcI 469 CTGCAG C^T[GA][CT]AG
SfiI 156 GGCCCAGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 544 CTCAAG C^T[CT][GA]AG
StyI 459 CCATGG C^C[AT][AT]GG
TaqI 77 TCGA T^CGA
TseI 468 GCTGC G^C[AT]GC
Tsp45I 703 GTGAC ^GT[GC]AC
TspRI 141 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 688 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 77 TCGA T^CGA
XmnI 483 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 13 enzymes) #
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AcsI 61 AAATTT [GA]^AATT[CT]
364 GAATTT
ApoI 61 AAATTT [GA]^AATT[CT]
364 GAATTT
BanI 27 GGTGCC G^G[CT][GA]CC
620 GGTACC
BglI 36 GCCCTCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
157 GCCCAGGCGGC
BsmBI 693 CGTCTC CGTCTC(1/5)
256 GAGACG
EaeI 104 TGGCCA [CT]^GGCC[GA]
572 TGGCCG
EcoNI 75 CCTCGACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
202 CCTCTGGAAGG
PpuMI 89 AGGTCCC [GA]G^G[AT]CC[CT]
500 GGGTCCT
SfaNI 299 GCATC GCATC(5/9)
796 GCATC
SmaI 280 CCCGGG CCC^GGG
698 CCCGGG
StuI 638 AGGCCT AGG^CCT
825 AGGCCT
XmaCI 280 CCCGGG C^CCGGG
698 CCCGGG
XmaI 280 CCCGGG C^CCGGG
698 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaI AvaII BanII BcnI BmyI
BpmI BsaJI BsgI BsiYI BslI
BsmAI BsoBI Bsp1286I BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HpaII ItaI
MaeII MboI MboII MflI MnlI
MspI MvaI MwoI NciI NdeII
NlaIV RsaI Sau3AI Sau96I ScrFI
Tsp509I XhoII