Restriction map for
pFN21AE1522
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccII AclI AflII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsiEI BsiWI BsmBI BspHI BssHII
BssSI BstEII BstPI BstUI BstXI
BstYI Bsu36I CelII CfoI CpoI
DraII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HhaI HinP1I
HpaI KasI KpnI KspI MfeI
MflI MluI MunI MvnI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NsiI PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI RcaI RsrII
SacII SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SwaI Tth111I Van91I
XbaI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AccIII 100 TCCGGA T^CCGGA
AcyI 512 GACGCC G[GA]^CG[CT]C
Asp700I 528 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 477 GAGCTC G[AT]GC[AT]^C
AvaI 419 CTCGAG C^[CT]CG[GA]G
AvaII 1101 GGACC G^G[AT]CC
BalI 263 TGGCCA TGG^CCA
BcnI 7 CCCGG CC^[GC]GG
BglI 523 GCCTTGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 335 CTCCAG CTGGAG(16/14)
BsaBI 274 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 512 GACGCC G[GA]^CG[CT]C
BsaI 1247 GAGACC GGTCTC(1/5)
BseAI 100 TCCGGA T^CCGGA
BseRI 352 GAGGAG GAGGAG(10/8)
BsgI 1359 CTGCAC GTGCAG(16/14)
BsiHKAI 477 GAGCTC G[AT]GC[AT]^C
BsmI 532 GCATTC GAATGC(1/-1)
BsoBI 419 CTCGAG C^[CT]CG[GA]G
Bsp1407I 656 TGTACA T^GTACA
BspDI 1471 ATCGAT AT^CGAT
BspEI 100 TCCGGA T^CCGGA
BspLU11I 798 ACATGT A^CATGT
BsrBI 74 CCGCTC CCGCTC(-3/-3)
BsrFI 43 ACCGGC [GA]^CCGG[CT]
BsrGI 656 TGTACA T^GTACA
Bst1107I 1352 GTATAC GTA^TAC
BstBI 1133 TTCGAA TT^CGAA
BstZ17I 1352 GTATAC GTA^TAC
Cfr10I 43 ACCGGC [GA]^CCGG[CT]
Cfr13I 1101 GGACC G^G[ACGT]CC
ClaI 1471 ATCGAT AT^CGAT
DraI 1432 TTTAAA TTT^AAA
DraIII 833 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 464 CCACGG C^C[GA][CT]GG
EcoRI 827 GAATTC G^AATTC
EcoT14I 500 CCTTGG C^C[AT][AT]GG
HgaI 512 GACGC GACGC(5/10)
Hin1I 512 GACGCC G[GA]^CG[CT]C
HindIII 673 AAGCTT A^AGCTT
MamI 274 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 263 TGGCCA TGG^CCA
MroI 100 TCCGGA T^CCGGA
MscI 263 TGGCCA TGG^CCA
MslI 465 CACGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 7 CCCGG CC^[GC]GG
NdeI 905 CATATG CA^TATG
NspI 798 ACATGT [GA]CATG^[CT]
NspV 1133 TTCGAA TT^CGAA
PaeR7I 419 CTCGAG C^TCGAG
PleI 1234 GAGTC GAGTC(4/5)
PshAI 1340 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 477 GAGCTC GAGCT^C
SalI 1183 GTCGAC G^TCGAC
SapI 1764 GCTCTTC GCTCTTC(1/4)
Sau96I 1101 GGACC G^G[ACGT]CC
SfuI 1133 TTCGAA TT^CGAA
SspBI 656 TGTACA T^GTACA
SspI 238 AATATT AAT^ATT
StuI 1718 AGGCCT AGG^CCT
StyI 500 CCTTGG C^C[AT][AT]GG
XcmI 851 CCAAAGTCTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 419 CTCGAG C^TCGAG
XmnI 528 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 55 GAATTT [GA]^AATT[CT]
827 GAATTC
AflIII 189 ACGTGT A^C[GA][CT]GT
798 ACATGT
ApoI 55 GAATTT [GA]^AATT[CT]
827 GAATTC
BanII 477 GAGCTC G[GA]GC[CT]^C
698 GAGCCC
BbsI 936 GTCTTC GAAGAC(2/6)
1148 GTCTTC
BfaI 229 CTAG C^TAG
509 CTAG
BmyI 477 GAGCTC G[AGT]GC[ACT]^C
698 GAGCCC
BpuAI 936 GTCTTC GAAGAC(2/6)
1148 GTCTTC
BsaWI 100 TCCGGA [AT]^CCGG[AT]
1687 TCCGGT
BsiYI 1092 CCTTCAGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1307 CCAAGAACGGG
BslI 1092 CCTTCAGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1307 CCAAGAACGGG
Bsp1286I 477 GAGCTC G[AGT]GC[ACT]^C
698 GAGCCC
BspMI 811 GCAGGT ACCTGC(4/8)
1317 GCAGGT
BstAPI 211 GCAGTCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
811 GCAGGTGCTGC
DpnII 871 GATC ^GATC
1474 GATC
EaeI 9 CGGCCA [CT]^GGCC[GA]
263 TGGCCA
HincII 159 GTTGAC GT[CT]^[GA]AC
1183 GTCGAC
HindII 159 GTTGAC GT[CT]^[GA]AC
1183 GTCGAC
HphI 747 GGTGA GGTGA(8/7)
946 GGTGA
MaeI 229 CTAG C^TAG
509 CTAG
MaeII 189 ACGT A^CGT
1640 ACGT
MboI 871 GATC ^GATC
1474 GATC
MseI 1156 TTAA T^TAA
1433 TTAA
NdeII 871 GATC ^GATC
1474 GATC
PvuII 652 CAGCTG CAG^CTG
742 CAGCTG
Sau3AI 871 GATC ^GATC
1474 GATC
Tru9I 1156 TTAA T^TAA
1433 TTAA
Tsp45I 424 GTGAC ^GT[GC]AC
1483 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AccI AciI AfaI AluI AlwNI
BbvI BsaJI BsmAI BsmFI BsrDI
BsrI BssKI BstF5I BstNI Cac8I
DdeI EarI EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
Ksp632I MaeIII MboII MnlI MspA1I
MspI MvaI MwoI NlaIII NlaIV
PstI RsaI ScrFI SfaNI SfcI
SmlI TaqI TfiI TseI Tsp509I
TspRI TthHB8I