Restriction map for
pFN21AE4340
##################################
# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BciVI BclI
BfrI BglII BlnI BsaAI BsaHI
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BsmI Bsp1407I BspEI BspHI
BspLU11I BsrBI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstYI
BstZ17I CpoI DraI DrdI DsaI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MfeI MflI MluI MroI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
Tsp45I XbaI XhoI XhoII XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AfaI 819 GTAC GT^AC
AocI 745 CCTGAGG CC^T[ACGT]AGG
Aor51HI 356 AGCGCT AGC^GCT
Asp700I 921 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 195 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 767 CTCGGG C^[CT]CG[GA]G
BalI 173 TGGCCA TGG^CCA
BbsI 940 GTCTTC GAAGAC(2/6)
BglI 175 GCCAACGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 940 GTCTTC GAAGAC(2/6)
BsaBI 525 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 118 GAGACG CGTCTC(1/5)
BsoBI 767 CTCGGG C^[CT]CG[GA]G
BspDI 208 ATCGAT AT^CGAT
BsrDI 426 CATTGC GCAATG(2/0)
BsrFI 215 ACCGGC [GA]^CCGG[CT]
BstXI 458 CCATTCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 745 CCTGAGG CC^T[ACGT]AGG
Cfr10I 215 ACCGGC [GA]^CCGG[CT]
ClaI 208 ATCGAT AT^CGAT
DraIII 725 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 829 CTCTTC CTCTTC(1/4)
Eco47III 356 AGCGCT AGC^GCT
Eco81I 745 CCTGAGG CC^T[ACGT]AGG
EcoNI 552 CCTCTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 602 GCGGG CCCGC(4/6)
Ksp632I 829 CTCTTC CTCTTC(1/4)
MamI 525 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 173 TGGCCA TGG^CCA
MscI 173 TGGCCA TGG^CCA
MseI 26 TTAA T^TAA
MspA1I 760 CAGCTG C[AC]G^C[GT]G
NlaIV 257 GGAACC GG[ACGT]^[ACGT]CC
PflFI 195 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 724 CCACACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 571 GAGTC GAGTC(4/5)
PpuMI 512 AGGACCT [GA]G^G[AT]CC[CT]
PstI 145 CTGCAG CTGCA^G
PvuII 760 CAGCTG CAG^CTG
RsaI 819 GTAC GT^AC
SapI 828 GCTCTTC GCTCTTC(1/4)
SexAI 104 ACCTGGT A^CC[AT]GGT
SfcI 145 CTGCAG C^T[GA][CT]AG
StuI 560 AGGCCT AGG^CCT
TfiI 878 GATTC G^A[AT]TC
Tru9I 26 TTAA T^TAA
Tsp509I 676 AATT ^AATT
Tth111I 195 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 724 CCACACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 924 CCAGTTCCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 921 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwI 327 GATCC GGATC(4/5)
992 GGATC
BanII 370 GAGCTC G[GA]GC[CT]^C
445 GAGCTC
BcgI 759 GCAGCTGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
969 GCAGGAGTGTCG
BsaJI 74 CCGGGG C^C[ACGT][ACGT]GG
852 CCCTGG
BsmAI 118 GAGAC GTCTC(1/5)
857 GAGAC
BsmFI 51 GGGAC GGGAC(10/14)
604 GGGAC
BspMI 438 ACCTGC ACCTGC(4/8)
841 GCAGGT
BssSI 889 CACGAG CACGAG(-5/-1)
373 CTCGTG
DraII 512 AGGACCT [GA]G^G[ACGT]CC[CT]
749 AGGGCCT
EaeI 173 TGGCCA [CT]^GGCC[GA]
800 TGGCCG
EcoO109I 512 AGGACCT [GA]G^G[ACGT]CC[CT]
749 AGGGCCT
HaeII 356 AGCGCT [GA]GCGC^[CT]
1013 AGCGCC
HgaI 120 GACGC GACGC(5/10)
864 GACGC
HinfI 571 GAGTC G^A[ACGT]TC
878 GATTC
HphI 187 TCACC GGTGA(8/7)
668 TCACC
MaeIII 164 GTAAC ^GT[ACGT]AC
308 GTTAC
SacI 370 GAGCTC GAGCT^C
445 GAGCTC
SmlI 220 CTCAAG C^T[CT][GA]AG
961 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AccII AciI AluI AspHI AvaII
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BsgI BsiHKAI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I CelII CfoI
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII ItaI MaeI MaeII MboI
MboII MnlI MslI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
Sau3AI Sau96I ScrFI SfaNI TaqI
TseI TspRI TthHB8I