Restriction map for
pFN21AB7903
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# NO CUT ENZYMES ( 187 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaII AviII AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DpnII DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FokI FseI FspI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MaeII MaeIII
MamI MboI MfeI MflI MluI
MluNI MroI MscI MseI MslI
MspA1I MunI NaeI NarI NcoI
NdeI NdeII NgoMIV NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsaI RsrII SacII SalI SapI
Sau3AI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tsp45I Tsp509I Tth111I XbaI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AccII 86 CGCG CG^CG
AluI 13 AGCT AG^CT
Aor51HI 117 AGCGCT AGC^GCT
AspHI 12 GAGCTC G[AT]GC[AT]^C
BsaI 165 GAGACC GGTCTC(1/5)
BsaWI 99 TCCGGT [AT]^CCGG[AT]
BsiHKAI 12 GAGCTC G[AT]GC[AT]^C
BsmAI 165 GAGAC GTCTC(1/5)
BsrI 267 ACTGG ACTGG(1/-1)
BstNI 235 CCAGG CC^[AT]GG
BstUI 86 CGCG CG^CG
BstXI 260 CCAAAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EarI 280 GAAGAG CTCTTC(1/4)
Eco47III 117 AGCGCT AGC^GCT
EcoRII 235 CCAGG ^CC[AT]GG
FauI 69 GCGGG CCCGC(4/6)
HaeII 117 AGCGCT [GA]GCGC^[CT]
HphI 102 GGTGA GGTGA(8/7)
Ksp632I 280 GAAGAG CTCTTC(1/4)
MboII 280 GAAGA GAAGA(8/7)
MvaI 235 CCAGG CC^[AT]GG
MvnI 86 CGCG CG^CG
PflMI 27 CCAGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 12 GAGCTC GAGCT^C
SfaNI 290 GCATC GCATC(5/9)
SmaI 109 CCCGGG CCC^GGG
TspRI 60 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 27 CCAGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 23 CCAGCCAGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 109 CCCGGG C^CCGGG
XmaI 109 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AciI 85 CCGC CCGC(-3/-1)
69 GCGG
AvaI 109 CCCGGG C^[CT]CG[GA]G
170 CTCGGG
BanII 12 GAGCTC G[GA]GC[CT]^C
139 GAGCCC
BbvI 93 GCAGC GCAGC(8/12)
205 GCTGC
BmyI 12 GAGCTC G[AGT]GC[ACT]^C
139 GAGCCC
BpmI 233 CTCCAG CTGGAG(16/14)
268 CTGGAG
BsmFI 112 GGGAC GGGAC(10/14)
107 GTCCC
BsoBI 109 CCCGGG C^[CT]CG[GA]G
170 CTCGGG
Bsp1286I 12 GAGCTC G[AGT]GC[ACT]^C
139 GAGCCC
CfoI 87 GCGC GCG^C
118 GCGC
Cfr13I 72 GGGCC G^G[ACGT]CC
174 GGGCC
DdeI 62 CTGAG C^T[ACGT]AG
161 CTAAG
EcoT14I 32 CCTTGG C^C[AT][AT]GG
247 CCAAGG
HhaI 87 GCGC GCG^C
118 GCGC
HinP1I 87 GCGC G^CGC
118 GCGC
Sau96I 72 GGGCC G^G[ACGT]CC
174 GGGCC
StyI 32 CCTTGG C^C[AT][AT]GG
247 CCAAGG
TaqI 10 TCGA T^CGA
16 TCGA
TseI 93 GCAGC G^C[AT]GC
205 GCTGC
TthHB8I 10 TCGA T^CGA
16 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
BcnI BsaJI BseRI BsiYI BslI
BssKI Cac8I Fnu4HI HaeIII HapII
HinfI HpaII ItaI MnlI MspI
MwoI NciI NlaIV PleI ScrFI