Restriction map for
pFN21AE4591
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# NO CUT ENZYMES ( 68 enzymes) #
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AccIII AclI AcsI AflII AflIII
Alw44I ApaLI ApoI AscI AseI
AsnI Asp700I AvrII BamHI BfaI
BfrI BlnI BsaBI BseAI BsiWI
Bsp1407I BspEI BspLU11I BsrGI BssHII
BstBI CpoI DraI DraIII EcoRI
EcoT22I HindIII HpaI MaeI MamI
MfeI MluI MroI MseI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PmeI PshAI
PshBI Psp1406I PvuI RsrII SbfI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tru9I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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Acc65I 599 GGTACC G^GTACC
AgeI 1180 ACCGGT A^CCGGT
AhdI 2338 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 599 GGTACC G^GTACC
AspEI 2338 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 2698 CACGTG CAC^GTG
BsaI 2564 GAGACC GGTCTC(1/5)
BsmBI 673 CGTCTC CGTCTC(1/5)
BsmI 3132 GAATGC GAATGC(1/-1)
BspDI 2626 ATCGAT AT^CGAT
BspHI 1829 TCATGA T^CATGA
BsrDI 918 CATTGC GCAATG(2/0)
Bst1107I 2098 GTATAC GTA^TAC
BstZ17I 2098 GTATAC GTA^TAC
ClaI 2626 ATCGAT AT^CGAT
DrdI 199 GACGGGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2338 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HincII 742 GTCGAC GT[CT]^[GA]AC
HindII 742 GTCGAC GT[CT]^[GA]AC
KpnI 599 GGTACC GGTAC^C
KspI 2333 CCGCGG CCGC^GG
NcoI 907 CCATGG C^CATGG
PinAI 1180 ACCGGT A^CCGGT
PmaCI 2698 CACGTG CAC^GTG
PmlI 2698 CACGTG CAC^GTG
RcaI 1829 TCATGA T^CATGA
SacI 799 GAGCTC GAGCT^C
SacII 2333 CCGCGG CCGC^GG
SalI 742 GTCGAC G^TCGAC
SapI 2436 GCTCTTC GCTCTTC(1/4)
ScaI 2864 AGTACT AGT^ACT
SexAI 2525 ACCTGGT A^CC[AT]GGT
SfiI 2013 GGCCGAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 1237 CTCAAG C^T[CT][GA]AG
TfiI 2285 GATTC G^A[AT]TC
Tsp509I 98 AATT ^AATT
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# TWO CUT ENZYMES ( 24 enzymes) #
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BbeI 2045 GGCGCC GGCGC^C
2113 GGCGCC
BbsI 1941 GAAGAC GAAGAC(2/6)
3268 GAAGAC
BbvCI 1626 CCTCAGC CCTCAGC(-5/-2)
2305 GCTGAGG
BcgI 2925 CGAGGGCCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2618 GCAACCGCATCG
BciVI 1666 GTATCC GTATCC(6/5)
3121 GTATCC
BclI 1175 TGATCA T^GATCA
2774 TGATCA
BglII 1007 AGATCT A^GATCT
2144 AGATCT
BlpI 936 GCTCAGC GC^T[ACGT]AGC
3054 GCTCAGC
Bpu1102I 936 GCTCAGC GC^T[ACGT]AGC
3054 GCTCAGC
BpuAI 1941 GAAGAC GAAGAC(2/6)
3268 GAAGAC
BsaAI 2698 CACGTG [CT]AC^GT[GA]
2770 TACGTG
BstEII 387 GGTCACC G^GT[ACGT]ACC
484 GGTGACC
BstPI 387 GGTCACC G^GT[ACGT]ACC
484 GGTGACC
CelII 936 GCTCAGC GC^T[ACGT]AGC
3054 GCTCAGC
EagI 554 CGGCCG C^GGCCG
960 CGGCCG
EclXI 554 CGGCCG C^GGCCG
960 CGGCCG
Eco52I 554 CGGCCG C^GGCCG
960 CGGCCG
EcoO65I 387 GGTCACC G^GT[ACGT]ACC
484 GGTGACC
EcoRV 946 GATATC GAT^ATC
2629 GATATC
FbaI 1175 TGATCA T^GATCA
2774 TGATCA
FseI 551 GGCCGGCC GGCCGG^CC
1272 GGCCGGCC
KasI 2045 GGCGCC G^GCGCC
2113 GGCGCC
NarI 2045 GGCGCC GG^CGCC
2113 GGCGCC
SfoI 2045 GGCGCC GGC^GCC
2113 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AatII AccI AccII AciI AcyI
AfaI AluI AlwI AlwNI AocI
Aor51HI ApaI AspHI AspI AvaI
AvaII AviII BalI BanI BanII
BbvI BcnI BglI BmyI BpmI
BsaHI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco47III Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
FspI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NspI PflFI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI StuI StyI TaqI
TseI Tsp45I TspRI Tth111I TthHB8I
Van91I XcmI XhoII XmaCI XmaI