Restriction map for
pFN21AE6320
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AlwI
AocI ApaI ApaLI AseI AsnI
Asp718I AspEI AvaII AviII AvrII
BalI BamHI BbsI BbvCI BcgI
BciVI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrGI BssSI Bst1107I BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FseI FspI
HgaI HincII HindII HindIII HpaI
KpnI MamI MfeI MflI MluI
MluNI MroI MscI MunI NcoI
NotI NruI NsiI NspI NspV
PacI PflMI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SalI SapI
SbfI ScaI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI TfiI
Van91I XbaI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcsI 874 AAATTT [GA]^AATT[CT]
AcyI 11 GGCGCC G[GA]^CG[CT]C
AflIII 1028 ACACGT A^C[GA][CT]GT
ApoI 874 AAATTT [GA]^AATT[CT]
AscI 71 GGCGCGCC GG^CGCGCC
Asp700I 1069 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 982 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 11 GGCGCC GGCGC^C
BbrPI 1029 CACGTG CAC^GTG
BclI 972 TGATCA T^GATCA
BfaI 34 CTAG C^TAG
BsaAI 1029 CACGTG [CT]AC^GT[GA]
BsaHI 11 GGCGCC G[GA]^CG[CT]C
BsaI 1195 GAGACC GGTCTC(1/5)
BsaWI 864 TCCGGT [AT]^CCGG[AT]
BseRI 1192 GAGGAG GAGGAG(10/8)
BsrBI 127 CCGCTC CCGCTC(-3/-3)
FbaI 972 TGATCA T^GATCA
Hin1I 11 GGCGCC G[GA]^CG[CT]C
HinfI 133 GAGTC G^A[ACGT]TC
KasI 11 GGCGCC G^GCGCC
KspI 23 CCGCGG CCGC^GG
MaeI 34 CTAG C^TAG
MaeII 1030 ACGT A^CGT
MaeIII 1090 GTGAC ^GT[ACGT]AC
NaeI 292 GCCGGC GCC^GGC
NarI 11 GGCGCC GG^CGCC
NdeI 614 CATATG CA^TATG
NgoMIV 292 GCCGGC G^CCGGC
NheI 33 GCTAGC G^CTAGC
PaeR7I 130 CTCGAG C^TCGAG
PflFI 982 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 133 GAGTC GAGTC(4/5)
PmaCI 1029 CACGTG CAC^GTG
PmlI 1029 CACGTG CAC^GTG
SacII 23 CCGCGG CCGC^GG
SexAI 1001 ACCTGGT A^CC[AT]GGT
SfoI 11 GGCGCC GGC^GCC
StuI 242 AGGCCT AGG^CCT
TaqI 131 TCGA T^CGA
Tsp45I 1090 GTGAC ^GT[GC]AC
Tsp509I 875 AATT ^AATT
Tth111I 982 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 131 TCGA T^CGA
XcmI 674 CCATCACAGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 130 CTCGAG C^TCGAG
XmnI 1069 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 162 GTAC GT^AC
1017 GTAC
AlwNI 370 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
997 CAGAACCTG
Aor51HI 36 AGCGCT AGC^GCT
359 AGCGCT
AspHI 172 GAGCAC G[AT]GC[AT]^C
1048 GAGCAC
BanI 11 GGCGCC G^G[CT][GA]CC
577 GGTGCC
BanII 283 GAGCCC G[GA]GC[CT]^C
436 GAGCCC
BsgI 217 CTGCAC GTGCAG(16/14)
950 GTGCAG
BsiHKAI 172 GAGCAC G[AT]GC[AT]^C
1048 GAGCAC
BsmAI 1195 GAGAC GTCTC(1/5)
728 GTCTC
BsmI 814 GAATGC GAATGC(1/-1)
936 GAATGC
BsrFI 143 ACCGGC [GA]^CCGG[CT]
292 GCCGGC
BstAPI 219 GCACCGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
369 GCAGGGCCTGC
Cfr10I 143 ACCGGC [GA]^CCGG[CT]
292 GCCGGC
DdeI 1041 CTCAG C^T[ACGT]AG
1124 CTAAG
DsaI 23 CCGCGG C^C[GA][CT]GG
109 CCACGG
Eco47III 36 AGCGCT AGC^GCT
359 AGCGCT
FauI 286 CCCGC CCCGC(4/6)
642 GCGGG
HphI 655 GGTGA GGTGA(8/7)
842 TCACC
MseI 593 TTAA T^TAA
1085 TTAA
MslI 523 CATCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
678 CACAGACATG
PstI 261 CTGCAG CTGCA^G
451 CTGCAG
RsaI 162 GTAC GT^AC
1017 GTAC
SfaNI 123 GATGC GCATC(5/9)
522 GCATC
SmlI 130 CTCGAG C^T[CT][GA]AG
355 CTCAAG
Tru9I 593 TTAA T^TAA
1085 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AccII AciI AluI AvaI BbvI
BcnI BmyI BpmI BsaJI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrI
BssHII BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DpnII DraII
EarI EcoO109I EcoRII Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HpaII ItaI Ksp632I MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfcI TseI
TspRI