Restriction map for
pFN21AE4424
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvrII BbeI BbrPI BbvCI
BcgI BciVI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaWI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrDI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FseI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MfeI MluI MroI MslI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RsrII SacII
SalI SapI SbfI ScaI SfcI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 529 GTAGAC GT^[AC][GT]AC
AccII 509 CGCG CG^CG
AlwNI 586 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 881 AGCGCT AGC^GCT
AviII 1223 TGCGCA TGC^GCA
BalI 821 TGGCCA TGG^CCA
BamHI 647 GGATCC G^GATCC
BbsI 696 GAAGAC GAAGAC(2/6)
BclI 368 TGATCA T^GATCA
BfaI 799 CTAG C^TAG
BpuAI 696 GAAGAC GAAGAC(2/6)
BsaI 443 GAGACC GGTCTC(1/5)
BsiEI 53 CGACCG CG[GA][CT]^CG
BsmBI 484 GAGACG CGTCTC(1/5)
BspHI 161 TCATGA T^CATGA
BsrBI 1029 CCGCTC CCGCTC(-3/-3)
BssSI 1249 CACGAG CACGAG(-5/-1)
BstUI 509 CGCG CG^CG
BstXI 945 CCAGCTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 731 CTAAG C^T[ACGT]AG
EaeI 821 TGGCCA [CT]^GGCC[GA]
EarI 165 GAAGAG CTCTTC(1/4)
Eco47III 881 AGCGCT AGC^GCT
FbaI 368 TGATCA T^GATCA
FspI 1223 TGCGCA TGC^GCA
HgaI 486 GACGC GACGC(5/10)
Ksp632I 165 GAAGAG CTCTTC(1/4)
MaeI 799 CTAG C^TAG
MluNI 821 TGGCCA TGG^CCA
MscI 821 TGGCCA TGG^CCA
MseI 977 TTAA T^TAA
MvnI 509 CGCG CG^CG
NcoI 521 CCATGG C^CATGG
PflMI 585 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 161 TCATGA T^CATGA
SexAI 446 ACCTGGT A^CC[AT]GGT
SmlI 100 CTCAAG C^T[CT][GA]AG
TaqI 281 TCGA T^CGA
Tru9I 977 TTAA T^TAA
TthHB8I 281 TCGA T^CGA
Van91I 585 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 261 GTAC GT^AC
393 GTAC
ApaI 679 GGGCCC GGGCC^C
1178 GGGCCC
AvaII 906 GGACC G^G[AT]CC
1014 GGACC
BsaBI 153 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
292 GATGTTGATC
BsmFI 688 GGGAC GGGAC(10/14)
905 GGGAC
BsrI 28 CCAGT ACTGG(1/-1)
1182 CCAGT
DraII 679 GGGCCCC [GA]G^G[ACGT]CC[CT]
1177 GGGGCCC
EcoNI 655 CCTCCAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
819 CCTGGCCAAGG
EcoO109I 679 GGGCCCC [GA]G^G[ACGT]CC[CT]
1177 GGGGCCC
EcoT14I 521 CCATGG C^C[AT][AT]GG
824 CCAAGG
HaeII 834 AGCGCC [GA]GCGC^[CT]
881 AGCGCT
MamI 153 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
292 GATGTTGATC
PvuII 763 CAGCTG CAG^CTG
946 CAGCTG
RsaI 261 GTAC GT^AC
393 GTAC
SmaI 710 CCCGGG CCC^GGG
1082 CCCGGG
SspI 16 AATATT AAT^ATT
328 AATATT
StyI 521 CCATGG C^C[AT][AT]GG
824 CCAAGG
TfiI 3 GAATC G^A[AT]TC
1091 GAATC
Tsp509I 84 AATT ^AATT
984 AATT
XmaCI 710 CCCGGG C^CCGGG
1082 CCCGGG
XmaI 710 CCCGGG C^CCGGG
1082 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaI
BanI BanII BbvI BcnI BmyI
BpmI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsoBI Bsp1286I
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DpnII DsaI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI SacI Sau3AI Sau96I ScrFI
SfaNI TseI Tsp45I TspRI XcmI
XhoII