Restriction map for
pFN21AE5221
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII AccI AclI AcyI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I AspEI AspHI AspI AvaI
AvaII AviII AvrII BamHI BanII
BbeI BbrPI BbvCI BcgI BclI
BcnI BfaI BfrI BglI BglII
BlnI BlpI BmyI BpmI Bpu1102I
BsaHI BsaI BsiHKAI BsiWI BsmFI
BsmI BsoBI Bsp1286I BspDI BspLU11I
BsrDI BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I CelII ClaI
CpoI DraI DraII DraIII DrdI
Eam1105I Eco47III EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI Hin1I HindIII HphI KasI
KspI MaeI MfeI MluI MslI
MunI NarI NciI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SpeI SphI Sse8387I SspI
StuI SwaI TaqI Tsp45I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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Acc65I 716 GGTACC G^GTACC
AccIII 722 TCCGGA T^CCGGA
AcsI 727 AAATTT [GA]^AATT[CT]
AlwI 442 GATCC GGATC(4/5)
AlwNI 175 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 513 CCTAAGG CC^T[ACGT]AGG
ApoI 727 AAATTT [GA]^AATT[CT]
Asp718I 716 GGTACC G^GTACC
BanI 716 GGTACC G^G[CT][GA]CC
BbsI 79 GTCTTC GAAGAC(2/6)
BpuAI 79 GTCTTC GAAGAC(2/6)
BsaAI 577 TACGTA [CT]AC^GT[GA]
BsaBI 489 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 722 TCCGGA [AT]^CCGG[AT]
BseAI 722 TCCGGA T^CCGGA
BsiEI 114 CGGCCG CG[GA][CT]^CG
BsmAI 233 GTCTC GTCTC(1/5)
BsmBI 232 CGTCTC CGTCTC(1/5)
Bsp1407I 349 TGTACA T^GTACA
BspEI 722 TCCGGA T^CCGGA
BspHI 708 TCATGA T^CATGA
BspMI 53 ACCTGC ACCTGC(4/8)
BsrBI 199 CCGCTC CCGCTC(-3/-3)
BsrFI 150 GCCGGC [GA]^CCGG[CT]
BsrGI 349 TGTACA T^GTACA
BssHII 242 GCGCGC G^CGCGC
BstYI 441 AGATCC [GA]^GATC[CT]
Bsu36I 513 CCTAAGG CC^T[ACGT]AGG
Cfr10I 150 GCCGGC [GA]^CCGG[CT]
DsaI 65 CCGTGG C^C[GA][CT]GG
EagI 114 CGGCCG C^GGCCG
EarI 733 GAAGAG CTCTTC(1/4)
EclXI 114 CGGCCG C^GGCCG
Eco52I 114 CGGCCG C^GGCCG
Eco81I 513 CCTAAGG CC^T[ACGT]AGG
HaeII 29 AGCGCC [GA]GCGC^[CT]
HgaI 231 GCGTC GACGC(5/10)
HincII 474 GTTAAC GT[CT]^[GA]AC
HindII 474 GTTAAC GT[CT]^[GA]AC
HpaI 474 GTTAAC GTT^AAC
KpnI 716 GGTACC GGTAC^C
Ksp632I 733 GAAGAG CTCTTC(1/4)
MamI 489 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 441 AGATCC [GA]^GATC[CT]
MroI 722 TCCGGA T^CCGGA
MspA1I 636 CAGCTG C[AC]G^C[GT]G
NaeI 150 GCCGGC GCC^GGC
NgoMIV 150 GCCGGC G^CCGGC
NspI 482 GCATGT [GA]CATG^[CT]
PleI 220 GAGTC GAGTC(4/5)
PvuII 636 CAGCTG CAG^CTG
RcaI 708 TCATGA T^CATGA
SmlI 402 CTTGAG C^T[CT][GA]AG
SnaBI 577 TACGTA TAC^GTA
SspBI 349 TGTACA T^GTACA
XhoII 441 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 29 enzymes) #
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AluI 179 AGCT AG^CT
637 AGCT
BalI 263 TGGCCA TGG^CCA
632 TGGCCA
BciVI 544 GGATAC GTATCC(6/5)
738 GTATCC
BseRI 25 GAGGAG GAGGAG(10/8)
320 GAGGAG
BsgI 172 GTGCAG GTGCAG(16/14)
413 GTGCAG
BstAPI 174 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
415 GCAGTGTATGC
BstF5I 255 CATCC GGATG(2/0)
495 CATCC
Cfr13I 158 GGGCC G^G[ACGT]CC
192 GGCCC
DpnII 442 GATC ^GATC
505 GATC
EcoT14I 556 CCAAGG C^C[AT][AT]GG
648 CCAAGG
FauI 228 CCCGC CCCGC(4/6)
156 GCGGG
FokI 255 CATCC GGATG(9/13)
495 CATCC
HapII 151 CCGG C^CGG
723 CCGG
HpaII 151 CCGG C^CGG
723 CCGG
MaeII 578 ACGT A^CGT
673 ACGT
MboI 442 GATC ^GATC
505 GATC
MluNI 263 TGGCCA TGG^CCA
632 TGGCCA
MscI 263 TGGCCA TGG^CCA
632 TGGCCA
MseI 298 TTAA T^TAA
475 TTAA
MspI 151 CCGG C^CGG
723 CCGG
NdeII 442 GATC ^GATC
505 GATC
NlaIV 140 GGCTCC GG[ACGT]^[ACGT]CC
716 GGTACC
PstI 55 CTGCAG CTGCA^G
584 CTGCAG
Sau3AI 442 GATC ^GATC
505 GATC
Sau96I 158 GGGCC G^G[ACGT]CC
192 GGCCC
SfcI 55 CTGCAG C^T[GA][CT]AG
584 CTGCAG
StyI 556 CCAAGG C^C[AT][AT]GG
648 CCAAGG
TfiI 90 GAATC G^A[AT]TC
519 GAATC
Tru9I 298 TTAA T^TAA
475 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AccII AciI AfaI BbvI BsaJI
BsiYI BslI BsrI BssKI BstNI
BstUI Cac8I CfoI DdeI EaeI
EcoRII Fnu4HI HaeIII HhaI HinP1I
HinfI ItaI MaeIII MboII MnlI
MvaI MvnI MwoI NlaIII RsaI
ScrFI SfaNI TseI Tsp509I TspRI