Restriction map for
pFN21AE1575
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# NO CUT ENZYMES ( 133 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BalI BamHI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrGI BsrI BssHII Bst1107I BstBI
BstEII BstPI BstXI BstYI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI Eam1105I Eco47III EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
MaeI MamI MfeI MflI MluI
MluNI MroI MscI MseI MunI
NaeI NcoI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SalI ScaI
SexAI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI SspBI
SspI StyI SwaI TfiI Tru9I
Tsp509I Tth111I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 407 GGTACC G^GTACC
AfaI 408 GTAC GT^AC
AlwI 308 GGATC GGATC(4/5)
AocI 672 CCTGAGG CC^T[ACGT]AGG
Asp718I 407 GGTACC G^GTACC
AvaI 39 CTCGGG C^[CT]CG[GA]G
BbsI 900 GTCTTC GAAGAC(2/6)
BpuAI 900 GTCTTC GAAGAC(2/6)
BsaAI 151 TACGTG [CT]AC^GT[GA]
BsaI 766 GAGACC GGTCTC(1/5)
BsaWI 254 ACCGGA [AT]^CCGG[AT]
BseRI 996 GAGGAG GAGGAG(10/8)
BsgI 853 GTGCAG GTGCAG(16/14)
BsiEI 117 CGGCCG CG[GA][CT]^CG
BsoBI 39 CTCGGG C^[CT]CG[GA]G
BsrDI 933 CATTGC GCAATG(2/0)
BsrFI 557 GCCGGT [GA]^CCGG[CT]
BssSI 760 CTCGTG CACGAG(-5/-1)
BstAPI 165 GCAGTTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 672 CCTGAGG CC^T[ACGT]AGG
Cfr10I 557 GCCGGT [GA]^CCGG[CT]
EagI 117 CGGCCG C^GGCCG
EarI 1002 GAAGAG CTCTTC(1/4)
EclXI 117 CGGCCG C^GGCCG
Eco52I 117 CGGCCG C^GGCCG
Eco81I 672 CCTGAGG CC^T[ACGT]AGG
EcoNI 447 CCTGAACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HinfI 639 GACTC G^A[ACGT]TC
KpnI 407 GGTACC GGTAC^C
Ksp632I 1002 GAAGAG CTCTTC(1/4)
MaeIII 1049 GTGAC ^GT[ACGT]AC
NdeI 634 CATATG CA^TATG
PflMI 198 CCAGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 639 GACTC GAGTC(4/5)
RsaI 408 GTAC GT^AC
SapI 1002 GAAGAGC GCTCTTC(1/4)
SbfI 775 CCTGCAGG CCTGCA^GG
SfaNI 929 GCATC GCATC(5/9)
Sse8387I 775 CCTGCAGG CCTGCA^GG
StuI 772 AGGCCT AGG^CCT
TaqI 179 TCGA T^CGA
Tsp45I 1049 GTGAC ^GT[GC]AC
TthHB8I 179 TCGA T^CGA
Van91I 198 CCAGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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ApaI 44 GGGCCC GGGCC^C
683 GGGCCC
AspHI 757 GAGCTC G[AT]GC[AT]^C
1005 GAGCTC
BbeI 12 GGCGCC GGCGC^C
216 GGCGCC
BcnI 110 CCCGG CC^[GC]GG
115 CCCGG
BsiHKAI 757 GAGCTC G[AT]GC[AT]^C
1005 GAGCTC
BsmBI 514 GAGACG CGTCTC(1/5)
601 GAGACG
BspMI 458 ACCTGC ACCTGC(4/8)
844 ACCTGC
EaeI 2 TGGCCG [CT]^GGCC[GA]
117 CGGCCG
KasI 12 GGCGCC G^GCGCC
216 GGCGCC
MaeII 152 ACGT A^CGT
377 ACGT
MboII 1002 GAAGA GAAGA(8/7)
901 TCTTC
MslI 99 CACCTCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
138 CATCGCCGTG
NarI 12 GGCGCC GG^CGCC
216 GGCGCC
NciI 110 CCCGG CC^[GC]GG
115 CCCGG
PpuMI 455 AGGACCT [GA]G^G[AT]CC[CT]
787 GGGACCT
PstI 607 CTGCAG CTGCA^G
776 CTGCAG
SacI 757 GAGCTC GAGCT^C
1005 GAGCTC
SfcI 607 CTGCAG C^T[GA][CT]AG
776 CTGCAG
SfoI 12 GGCGCC GGC^GCC
216 GGCGCC
XcmI 357 CCAGAGGATGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
914 CCATCCCCATCGTGG
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# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AcyI AluI AvaII
BanI BanII BbvI BglI BmyI
BpmI BsaHI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII HphI ItaI KspI
MboI MnlI MspA1I MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
SacII Sau3AI Sau96I ScrFI TseI
TspRI