Restriction map for
pFN21AE1348
##################################
# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BbeI BbrPI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI BspDI BspEI BspHI BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraIII DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HhaI Hin1I HinP1I HindIII
HpaI KasI KpnI KspI MamI
MluI MluNI MroI MscI MslI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SmlI SpeI Sse8387I SspI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AcsI 1193 AAATTC [GA]^AATT[CT]
AflIII 1283 ACATGT A^C[GA][CT]GT
AluI 429 AGCT AG^CT
AlwNI 389 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 1193 AAATTC [GA]^AATT[CT]
AseI 736 ATTAAT AT^TAAT
AsnI 736 ATTAAT AT^TAAT
Asp700I 1189 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 549 GGATCC G^GATCC
BanI 355 GGTGCC G^G[CT][GA]CC
BanII 22 GGGCTC G[GA]GC[CT]^C
BbsI 105 GAAGAC GAAGAC(2/6)
BbvCI 319 GCTGAGG CCTCAGC(-5/-2)
BcgI 1393 GCATGCAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BmyI 22 GGGCTC G[AGT]GC[ACT]^C
BpmI 905 CTCCAG CTGGAG(16/14)
BpuAI 105 GAAGAC GAAGAC(2/6)
BsaAI 273 TACGTA [CT]AC^GT[GA]
BsaWI 11 ACCGGA [AT]^CCGG[AT]
BsgI 936 CTGCAC GTGCAG(16/14)
BsmFI 1258 GGGAC GGGAC(10/14)
Bsp1286I 22 GGGCTC G[AGT]GC[ACT]^C
BspLU11I 1283 ACATGT A^CATGT
BstBI 364 TTCGAA TT^CGAA
Cac8I 1393 GCATGC GC[ACGT]^[ACGT]GC
DraI 151 TTTAAA TTT^AAA
DraII 264 AGGTCCT [GA]G^G[ACGT]CC[CT]
DrdI 325 GACAACACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1215 GAAGAG CTCTTC(1/4)
EcoO109I 264 AGGTCCT [GA]G^G[ACGT]CC[CT]
HapII 12 CCGG C^CGG
HgaI 316 GACGC GACGC(5/10)
HincII 1165 GTCAAC GT[CT]^[GA]AC
HindII 1165 GTCAAC GT[CT]^[GA]AC
HpaII 12 CCGG C^CGG
Ksp632I 1215 GAAGAG CTCTTC(1/4)
MfeI 143 CAATTG C^AATTG
MspA1I 428 CAGCTG C[AC]G^C[GT]G
MspI 12 CCGG C^CGG
MunI 143 CAATTG C^AATTG
NdeI 971 CATATG CA^TATG
NspV 364 TTCGAA TT^CGAA
PpuMI 264 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 736 ATTAAT AT^TAAT
PvuII 428 CAGCTG CAG^CTG
SfuI 364 TTCGAA TT^CGAA
SnaBI 273 TACGTA TAC^GTA
SphI 1393 GCATGC GCATG^C
StuI 893 AGGCCT AGG^CCT
XmnI 1189 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AvaII 265 GGTCC G^G[AT]CC
1259 GGACC
BfaI 292 CTAG C^TAG
982 CTAG
BsaI 89 GAGACC GGTCTC(1/5)
1008 GAGACC
BsiYI 1066 CCTATTGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1123 CCTATAACTGG
BslI 1066 CCTATTGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1123 CCTATAACTGG
Bsp1407I 964 TGTACA T^GTACA
1016 TGTACA
BsrGI 964 TGTACA T^GTACA
1016 TGTACA
BstF5I 434 CATCC GGATG(2/0)
887 CATCC
BstYI 197 GGATCT [GA]^GATC[CT]
549 GGATCC
Cfr13I 265 GGTCC G^G[ACGT]CC
1259 GGACC
DdeI 320 CTGAG C^T[ACGT]AG
1345 CTCAG
FokI 434 CATCC GGATG(9/13)
887 CATCC
HaeIII 894 GGCC GG^CC
1324 GGCC
HphI 1267 GGTGA GGTGA(8/7)
1359 GGTGA
MaeI 292 CTAG C^TAG
982 CTAG
MaeII 274 ACGT A^CGT
845 ACGT
MflI 197 GGATCT [GA]^GATC[CT]
549 GGATCC
MwoI 49 GCTGTCCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
421 GCTGTGGCAGC
NspI 1283 ACATGT [GA]CATG^[CT]
1393 GCATGC
Sau96I 265 GGTCC G^G[ACGT]CC
1259 GGACC
SfaNI 748 GATGC GCATC(5/9)
433 GCATC
SspBI 964 TGTACA T^GTACA
1016 TGTACA
Tsp45I 8 GTGAC ^GT[GC]AC
722 GTCAC
TspRI 724 CACTG CA[GC]TG[ACGT][ACGT]^
1347 CAGTG
XhoII 197 GGATCT [GA]^GATC[CT]
549 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AfaI AlwI BbvI BsaJI BsmAI
BspMI BsrI BssKI BstNI DpnII
EcoRII EcoT14I Fnu4HI HinfI ItaI
MaeIII MboI MboII MnlI MseI
MvaI NdeII NlaIII NlaIV PstI
RsaI Sau3AI ScrFI SfcI StyI
TaqI TfiI Tru9I TseI Tsp509I
TthHB8I