Restriction map for
pFN21AE1401
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# NO CUT ENZYMES ( 192 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsiYI BslI
BsmAI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DdeI
DraI DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FseI FspI Hin1I
HincII HindII HindIII HinfI HpaI
HphI KasI KpnI Ksp632I KspI
MaeI MaeII MaeIII MamI MfeI
MflI MluI MluNI MroI MscI
MseI MslI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I Tsp45I Tth111I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AlwI 277 GATCC GGATC(4/5)
Aor51HI 106 AGCGCT AGC^GCT
ApaI 94 GGGCCC GGGCC^C
BclI 185 TGATCA T^GATCA
BcnI 34 CCCGG CC^[GC]GG
BsmFI 31 GTCCC GGGAC(10/14)
BssHII 56 GCGCGC G^CGCGC
BstNI 91 CCAGG CC^[AT]GG
DraII 93 AGGGCCC [GA]G^G[ACGT]CC[CT]
Eco47III 106 AGCGCT AGC^GCT
EcoO109I 93 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRII 91 CCAGG ^CC[AT]GG
EcoT14I 227 CCAAGG C^C[AT][AT]GG
FbaI 185 TGATCA T^GATCA
HaeII 106 AGCGCT [GA]GCGC^[CT]
HapII 35 CCGG C^CGG
HgaI 84 GACGC GACGC(5/10)
HpaII 35 CCGG C^CGG
MboII 252 GAAGA GAAGA(8/7)
MspA1I 119 CCGCTG C[AC]G^C[GT]G
MspI 35 CCGG C^CGG
MvaI 91 CCAGG CC^[AT]GG
NciI 34 CCCGG CC^[GC]GG
NlaIV 94 GGGCCC GG[ACGT]^[ACGT]CC
SacI 45 GAGCTC GAGCT^C
StyI 227 CCAAGG C^C[AT][AT]GG
TaqI 43 TCGA T^CGA
TspRI 220 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 43 TCGA T^CGA
XcmI 227 CCAAGGTGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcsI 266 AAATTT [GA]^AATT[CT]
314 AAATTT
AluI 46 AGCT AG^CT
137 AGCT
ApoI 266 AAATTT [GA]^AATT[CT]
314 AAATTT
AspHI 45 GAGCTC G[AT]GC[AT]^C
222 GTGCTC
AvaII 37 GGTCC G^G[AT]CC
112 GGTCC
BanII 45 GAGCTC G[GA]GC[CT]^C
94 GGGCCC
BsiHKAI 45 GAGCTC G[AT]GC[AT]^C
222 GTGCTC
BssKI 34 CCCGG ^CC[ACGT]GG
91 CCAGG
BstF5I 175 CATCC GGATG(2/0)
306 CATCC
Cac8I 56 GCGCGC GC[ACGT]^[ACGT]GC
103 GCCAGC
DpnII 186 GATC ^GATC
277 GATC
FokI 175 CATCC GGATG(9/13)
306 CATCC
MboI 186 GATC ^GATC
277 GATC
MnlI 6 CCTC CCTC(7/6)
245 CCTC
NdeII 186 GATC ^GATC
277 GATC
Sau3AI 186 GATC ^GATC
277 GATC
ScrFI 34 CCCGG CC^[ACGT]GG
91 CCAGG
Tsp509I 267 AATT ^AATT
315 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AccII AciI BbvI BmyI BsaJI
Bsp1286I BstUI CfoI Cfr13I Fnu4HI
HaeIII HhaI HinP1I ItaI MvnI
MwoI Sau96I TseI