Restriction map for
pFN21AE4558
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI Alw44I AlwNI
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspI AviII BamHI
BbeI BbrPI BbvCI BcgI BfrI
BglI BlpI Bpu1102I BsaHI BsaI
BsaWI BseAI BsgI BsiWI BsmBI
BspDI BspEI BspLU11I BspMI BsrBI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoT22I FseI
FspI HaeII HhaI Hin1I HinP1I
HpaI KasI KpnI MfeI MluI
MroI MunI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PstI PvuI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccII 1047 CGCG CG^CG
AhdI 1380 GACATCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 271 GGGCCC GGGCC^C
AspEI 1380 GACATCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 609 CCCGAG C^[CT]CG[GA]G
AvrII 714 CCTAGG C^CTAGG
BalI 899 TGGCCA TGG^CCA
BciVI 1507 GGATAC GTATCC(6/5)
BglII 686 AGATCT A^GATCT
BlnI 714 CCTAGG C^CTAGG
BpmI 1253 CTGGAG CTGGAG(16/14)
BsiEI 1614 CGACCG CG[GA][CT]^CG
BsmI 1339 GAATGC GAATGC(1/-1)
BsoBI 609 CCCGAG C^[CT]CG[GA]G
BspHI 1280 TCATGA T^CATGA
BstEII 796 GGTGACC G^GT[ACGT]ACC
BstPI 796 GGTGACC G^GT[ACGT]ACC
BstUI 1047 CGCG CG^CG
EaeI 899 TGGCCA [CT]^GGCC[GA]
Eam1105I 1380 GACATCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 796 GGTGACC G^GT[ACGT]ACC
EcoRV 535 GATATC GAT^ATC
FauI 1045 CCCGC CCCGC(4/6)
HgaI 767 GCGTC GACGC(5/10)
KspI 1046 CCGCGG CCGC^GG
MluNI 899 TGGCCA TGG^CCA
MscI 899 TGGCCA TGG^CCA
MvnI 1047 CGCG CG^CG
NcoI 542 CCATGG C^CATGG
NdeI 925 CATATG CA^TATG
NspI 870 GCATGC [GA]CATG^[CT]
PpuMI 1517 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 1573 GACATTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 960 CAGCTG CAG^CTG
RcaI 1280 TCATGA T^CATGA
SacII 1046 CCGCGG CCGC^GG
SfcI 306 CTACAG C^T[GA][CT]AG
SphI 870 GCATGC GCATG^C
SspI 829 AATATT AAT^ATT
TfiI 980 GATTC G^A[AT]TC
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# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcsI 1144 AAATTC [GA]^AATT[CT]
1709 AAATTT
AflIII 458 ACACGT A^C[GA][CT]GT
1496 ACGTGT
ApoI 1144 AAATTC [GA]^AATT[CT]
1709 AAATTT
BanI 1050 GGTGCC G^G[CT][GA]CC
1398 GGTGCC
BclI 92 TGATCA T^GATCA
623 TGATCA
BcnI 42 CCCGG CC^[GC]GG
1293 CCGGG
BfaI 715 CTAG C^TAG
749 CTAG
BsaAI 459 CACGTA [CT]AC^GT[GA]
1417 TACGTG
BsaBI 51 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
311 GATGGCTATC
BseRI 158 GAGGAG GAGGAG(10/8)
1988 GAGGAG
BsmAI 1571 GAGAC GTCTC(1/5)
362 GTCTC
BsrDI 1656 CATTGC GCAATG(2/0)
1300 GCAATG
DraII 271 GGGCCCT [GA]G^G[ACGT]CC[CT]
1517 AGGACCC
DrdI 394 GACCAGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
760 GACCTGAGCGTC
DsaI 542 CCATGG C^C[GA][CT]GG
1046 CCGCGG
EarI 119 CTCTTC CTCTTC(1/4)
672 GAAGAG
EcoO109I 271 GGGCCCT [GA]G^G[ACGT]CC[CT]
1517 AGGACCC
FbaI 92 TGATCA T^GATCA
623 TGATCA
HapII 43 CCGG C^CGG
1293 CCGG
HincII 523 GTCAAC GT[CT]^[GA]AC
1694 GTCAAC
HindII 523 GTCAAC GT[CT]^[GA]AC
1694 GTCAAC
HindIII 1322 AAGCTT A^AGCTT
1795 AAGCTT
HpaII 43 CCGG C^CGG
1293 CCGG
Ksp632I 119 CTCTTC CTCTTC(1/4)
672 GAAGAG
MaeI 715 CTAG C^TAG
749 CTAG
MamI 51 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
311 GATGGCTATC
MslI 627 CATCCTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
871 CATGCTTATG
MspA1I 960 CAGCTG C[AC]G^C[GT]G
1046 CCGCGG
MspI 43 CCGG C^CGG
1293 CCGG
NciI 42 CCCGG CC^[GC]GG
1293 CCGGG
TaqI 1056 TCGA T^CGA
1613 TCGA
TthHB8I 1056 TCGA T^CGA
1613 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AfaI AluI AlwI Asp700I
AspHI AvaII BanII BbsI BbvI
BmyI BpuAI BsaJI BsiHKAI BsiYI
BslI BsmFI Bsp1286I Bsp1407I BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeII MaeIII MboI
MboII MflI MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SspBI StyI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII XmnI