Restriction map for
pFN21AE6230
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# NO CUT ENZYMES ( 135 enzymes) #
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AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BbrPI
BbvCI BcgI BclI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspEI
BspHI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I CelII DraI DraII DrdI
DsaI EagI Eam1105I EclXI Eco52I
EcoNI EcoO109I EcoO65I EcoRV EcoT14I
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MaeII MaeIII MamI MfeI MluI
MroI MseI MslI MunI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StyI
SwaI Tru9I Tsp45I Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 238 GTAGAC GT^[AC][GT]AC
AlwNI 590 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 871 CCTGAGG CC^T[ACGT]AGG
Aor51HI 170 AGCGCT AGC^GCT
AspHI 112 GTGCTC G[AT]GC[AT]^C
BalI 2 TGGCCA TGG^CCA
BanII 62 GAGCCC G[GA]GC[CT]^C
BbeI 137 GGCGCC GGCGC^C
BbsI 310 GAAGAC GAAGAC(2/6)
BciVI 86 GTATCC GTATCC(6/5)
BglII 387 AGATCT A^GATCT
BpuAI 310 GAAGAC GAAGAC(2/6)
BsaI 181 GAGACC GGTCTC(1/5)
BsiHKAI 112 GTGCTC G[AT]GC[AT]^C
BspDI 789 ATCGAT AT^CGAT
BspMI 581 GCAGGT ACCTGC(4/8)
BsrBI 150 GAGCGG CCGCTC(-3/-3)
BsrFI 47 GCCGGC [GA]^CCGG[CT]
BsrI 524 ACTGG ACTGG(1/-1)
BstAPI 589 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 871 CCTGAGG CC^T[ACGT]AGG
Cfr10I 47 GCCGGC [GA]^CCGG[CT]
ClaI 789 ATCGAT AT^CGAT
CpoI 153 CGGTCCG CG^G[AT]CCG
DraIII 471 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 2 TGGCCA [CT]^GGCC[GA]
EarI 737 GAAGAG CTCTTC(1/4)
Eco47III 170 AGCGCT AGC^GCT
Eco81I 871 CCTGAGG CC^T[ACGT]AGG
EcoRI 484 GAATTC G^AATTC
KasI 137 GGCGCC G^GCGCC
Ksp632I 737 GAAGAG CTCTTC(1/4)
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MspA1I 415 CAGCGG C[AC]G^C[GT]G
NaeI 47 GCCGGC GCC^GGC
NarI 137 GGCGCC GG^CGCC
NgoMIV 47 GCCGGC G^CCGGC
PstI 537 CTGCAG CTGCA^G
RsrII 153 CGGTCCG CG^G[AT]CCG
SfoI 137 GGCGCC GGC^GCC
SspI 808 AATATT AAT^ATT
StuI 716 AGGCCT AGG^CCT
TaqI 790 TCGA T^CGA
TthHB8I 790 TCGA T^CGA
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 117 CGCG CG^CG
194 CGCG
AcsI 215 AAATTC [GA]^AATT[CT]
484 GAATTC
AcyI 79 GGCGTC G[GA]^CG[CT]C
137 GGCGCC
AfaI 207 GTAC GT^AC
349 GTAC
AflIII 234 ACATGT A^C[GA][CT]GT
422 ACATGT
AluI 594 AGCT AG^CT
782 AGCT
ApoI 215 AAATTC [GA]^AATT[CT]
484 GAATTC
AvaI 129 CCCGAG C^[CT]CG[GA]G
178 CCCGAG
BamHI 628 GGATCC G^GATCC
765 GGATCC
BanI 137 GGCGCC G^G[CT][GA]CC
160 GGCACC
BpmI 433 CTGGAG CTGGAG(16/14)
525 CTGGAG
BsaHI 79 GGCGTC G[GA]^CG[CT]C
137 GGCGCC
BsoBI 129 CCCGAG C^[CT]CG[GA]G
178 CCCGAG
BspLU11I 234 ACATGT A^CATGT
422 ACATGT
BstUI 117 CGCG CG^CG
194 CGCG
HaeII 137 GGCGCC [GA]GCGC^[CT]
170 AGCGCT
HgaI 80 GCGTC GACGC(5/10)
200 GCGTC
Hin1I 79 GGCGTC G[GA]^CG[CT]C
137 GGCGCC
MvnI 117 CGCG CG^CG
194 CGCG
NspI 234 ACATGT [GA]CATG^[CT]
422 ACATGT
RsaI 207 GTAC GT^AC
349 GTAC
SfcI 537 CTGCAG C^T[GA][CT]AG
834 CTACAG
XcmI 66 CCAACTCTGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
773 CCATAAAAGAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AlwI AvaII BbvI BcnI
BfaI BmyI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MboI MboII MflI MnlI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
TfiI TseI Tsp509I TspRI XhoII