Restriction map for
pFN21AB5020
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI AspEI
AspI AviII AvrII BamHI BbeI
BbrPI BcgI BclI BfrI BglI
BlnI BlpI Bpu1102I BsaBI BseAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BsrBI BsrDI BsrGI
BssSI Bst1107I BstBI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI Eam1105I Eco47III Eco81I
EcoRI EcoRV FbaI FseI FspI
HincII HindII HpaI KasI KspI
MamI MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SmaI
SnaBI SpeI Sse8387I SspBI StuI
SwaI Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcsI 252 GAATTT [GA]^AATT[CT]
AcyI 61 GGCGTC G[GA]^CG[CT]C
AflIII 1103 ACATGT A^C[GA][CT]GT
AgeI 1647 ACCGGT A^CCGGT
AlwNI 1195 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 252 GAATTT [GA]^AATT[CT]
BbsI 19 GTCTTC GAAGAC(2/6)
BciVI 188 GGATAC GTATCC(6/5)
BfaI 865 CTAG C^TAG
BglII 491 AGATCT A^GATCT
BpuAI 19 GTCTTC GAAGAC(2/6)
BsaAI 721 TACGTG [CT]AC^GT[GA]
BsaHI 61 GGCGTC G[GA]^CG[CT]C
BsgI 1351 GTGCAG GTGCAG(16/14)
BspLU11I 1103 ACATGT A^CATGT
BspMI 850 GCAGGT ACCTGC(4/8)
BsrFI 1647 ACCGGT [GA]^CCGG[CT]
BssHII 9 GCGCGC G^CGCGC
BstAPI 212 GCAGGAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 1647 ACCGGT [GA]^CCGG[CT]
EcoNI 603 CCTGGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1255 ATGCAT ATGCA^T
FauI 1273 GCGGG CCCGC(4/6)
Hin1I 61 GGCGTC G[GA]^CG[CT]C
HindIII 1434 AAGCTT A^AGCTT
MaeI 865 CTAG C^TAG
MaeII 722 ACGT A^CGT
MseI 1616 TTAA T^TAA
NsiI 1255 ATGCAT ATGCA^T
PinAI 1647 ACCGGT A^CCGGT
PstI 210 CTGCAG CTGCA^G
PvuII 412 CAGCTG CAG^CTG
SapI 1300 GAAGAGC GCTCTTC(1/4)
SfcI 210 CTGCAG C^T[GA][CT]AG
SgrAI 1646 CACCGGTG C[GA]^CCGG[CT]G
SmlI 980 CTCAAG C^T[CT][GA]AG
SphI 1013 GCATGC GCATG^C
SspI 920 AATATT AAT^ATT
TaqI 23 TCGA T^CGA
Tru9I 1616 TTAA T^TAA
Tsp509I 253 AATT ^AATT
TthHB8I 23 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 824 GGTACC G^GTACC
1394 GGTACC
AccII 10 CGCG CG^CG
12 CGCG
Asp700I 342 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1482 GAAGGACTTC
Asp718I 824 GGTACC G^GTACC
1394 GGTACC
AspHI 219 GTGCTC G[AT]GC[AT]^C
1492 GTGCTC
AvaI 802 CCCGAG C^[CT]CG[GA]G
1045 CCCGAG
BalI 996 TGGCCA TGG^CCA
1064 TGGCCA
BanII 765 GGGCTC G[GA]GC[CT]^C
1303 GAGCCC
BbvCI 1311 CCTCAGC CCTCAGC(-5/-2)
1177 GCTGAGG
BpmI 316 CTCCAG CTGGAG(16/14)
637 CTGGAG
BsaI 226 GAGACC GGTCTC(1/5)
1653 GGTCTC
BsaWI 649 ACCGGA [AT]^CCGG[AT]
1647 ACCGGT
BsiHKAI 219 GTGCTC G[AT]GC[AT]^C
1492 GTGCTC
BsmAI 226 GAGAC GTCTC(1/5)
1654 GTCTC
BsoBI 802 CCCGAG C^[CT]CG[GA]G
1045 CCCGAG
BstUI 10 CGCG CG^CG
12 CGCG
DsaI 714 CCATGG C^C[GA][CT]GG
792 CCATGG
EagI 14 CGGCCG C^GGCCG
69 CGGCCG
EclXI 14 CGGCCG C^GGCCG
69 CGGCCG
Eco52I 14 CGGCCG C^GGCCG
69 CGGCCG
EcoT14I 714 CCATGG C^C[AT][AT]GG
792 CCATGG
HaeII 42 GGCGCT [GA]GCGC^[CT]
90 GGCGCT
HgaI 3 GACGC GACGC(5/10)
62 GCGTC
KpnI 824 GGTACC GGTAC^C
1394 GGTACC
MluNI 996 TGGCCA TGG^CCA
1064 TGGCCA
MscI 996 TGGCCA TGG^CCA
1064 TGGCCA
MslI 927 CATGGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1014 CATGCTGGTG
MspA1I 50 CAGCGG C[AC]G^C[GT]G
412 CAGCTG
MvnI 10 CGCG CG^CG
12 CGCG
NcoI 714 CCATGG C^CATGG
792 CCATGG
NspI 1013 GCATGC [GA]CATG^[CT]
1103 ACATGT
PflMI 368 CCAACACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1512 CCAGCACATGG
PpuMI 860 GGGTCCT [GA]G^G[AT]CC[CT]
1380 AGGTCCT
StyI 714 CCATGG C^C[AT][AT]GG
792 CCATGG
Van91I 368 CCAACACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1512 CCAGCACATGG
XcmI 368 CCAACACCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
999 CCATGACTGGGGTGG
XmnI 342 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1482 GAAGGACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanI BbvI BcnI BmyI BsaJI
BseRI BsiEI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstEII BstF5I
BstNI BstPI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoO65I EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MflI MnlI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI TfiI TseI
Tsp45I TspRI XhoII