Restriction map for
pFN21AE6447
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# NO CUT ENZYMES ( 125 enzymes) #
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AatII AccI AccII AccIII AcyI
AflII AflIII AgeI AhdI Aor51HI
ApaI AscI AseI AsnI Asp700I
AspEI AviII AvrII BbeI BbrPI
BclI BfrI BglI BlnI BsaAI
BsaBI BsaHI BsaWI BseAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I ClaI DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HgaI Hin1I KasI Ksp632I
KspI MaeIII MamI MfeI MluI
MroI MunI MvnI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI PvuI RcaI SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tsp45I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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Acc65I 184 GGTACC G^GTACC
AclI 446 AACGTT AA^CGTT
AfaI 185 GTAC GT^AC
Alw44I 78 GTGCAC G^TGCAC
AocI 784 CCTGAGG CC^T[ACGT]AGG
ApaLI 78 GTGCAC G^TGCAC
Asp718I 184 GGTACC G^GTACC
AspI 808 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 977 CTCGGG C^[CT]CG[GA]G
AvaII 90 GGTCC G^G[AT]CC
BamHI 858 GGATCC G^GATCC
BbsI 1194 GAAGAC GAAGAC(2/6)
BcgI 61 CGACCGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 832 GTATCC GTATCC(6/5)
BcnI 83 CCGGG CC^[GC]GG
BglII 953 AGATCT A^GATCT
BlpI 45 GCTCAGC GC^T[ACGT]AGC
BpmI 889 CTGGAG CTGGAG(16/14)
Bpu1102I 45 GCTCAGC GC^T[ACGT]AGC
BpuAI 1194 GAAGAC GAAGAC(2/6)
BsaI 902 GAGACC GGTCTC(1/5)
BsgI 1357 CTGCAC GTGCAG(16/14)
BsiEI 61 CGACCG CG[GA][CT]^CG
BsmI 1379 GCATTC GAATGC(1/-1)
BsoBI 977 CTCGGG C^[CT]CG[GA]G
BsrDI 534 CATTGC GCAATG(2/0)
BsrFI 641 ACCGGC [GA]^CCGG[CT]
BstXI 816 CCAGTGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 784 CCTGAGG CC^T[ACGT]AGG
CelII 45 GCTCAGC GC^T[ACGT]AGC
Cfr10I 641 ACCGGC [GA]^CCGG[CT]
CpoI 89 CGGTCCG CG^G[AT]CCG
DraI 253 TTTAAA TTT^AAA
DraIII 1126 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 784 CCTGAGG CC^T[ACGT]AGG
HaeII 1085 GGCGCT [GA]GCGC^[CT]
HincII 123 GTTAAC GT[CT]^[GA]AC
HindII 123 GTTAAC GT[CT]^[GA]AC
HindIII 1279 AAGCTT A^AGCTT
HpaI 123 GTTAAC GTT^AAC
KpnI 184 GGTACC GGTAC^C
MslI 1360 CACTATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 83 CCGGG CC^[GC]GG
NdeI 208 CATATG CA^TATG
NspI 1106 GCATGT [GA]CATG^[CT]
PflFI 808 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 446 AACGTT AA^CGTT
PstI 1024 CTGCAG CTGCA^G
RsaI 185 GTAC GT^AC
RsrII 89 CGGTCCG CG^G[AT]CCG
SmlI 1229 CTCAAG C^T[CT][GA]AG
StuI 774 AGGCCT AGG^CCT
TaqI 798 TCGA T^CGA
Tth111I 808 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 798 TCGA T^CGA
XcmI 816 CCAGTGTCATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AlwNI 52 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
883 CAGGCCCTG
AspHI 78 GTGCAC G[AT]GC[AT]^C
1371 GAGCAC
BanI 184 GGTACC G^G[CT][GA]CC
1131 GGTGCC
BbvCI 202 CCTCAGC CCTCAGC(-5/-2)
966 CCTCAGC
BsiHKAI 78 GTGCAC G[AT]GC[AT]^C
1371 GAGCAC
CfoI 71 GCGC GCG^C
1086 GCGC
EcoT14I 495 CCAAGG C^C[AT][AT]GG
1118 CCAAGG
HapII 83 CCGG C^CGG
642 CCGG
HhaI 71 GCGC GCG^C
1086 GCGC
HinP1I 71 GCGC G^CGC
1086 GCGC
HpaII 83 CCGG C^CGG
642 CCGG
MaeII 258 ACGT A^CGT
447 ACGT
MspA1I 246 CAGCTG C[AC]G^C[GT]G
1354 CAGCTG
MspI 83 CCGG C^CGG
642 CCGG
MwoI 45 GCTCAGCCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
71 GCGCTTCGTGC
PvuII 246 CAGCTG CAG^CTG
1354 CAGCTG
SfaNI 565 GATGC GCATC(5/9)
1320 GATGC
SfcI 1024 CTGCAG C^T[GA][CT]AG
1220 CTACAG
StyI 495 CCAAGG C^C[AT][AT]GG
1118 CCAAGG
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# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
BalI BanII BbvI BfaI BmyI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeI MboI MboII MflI
MluNI MnlI MscI MseI MvaI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI TfiI Tru9I TseI
Tsp509I TspRI XhoII