Restriction map for
pFN21AE4543
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BcgI BcnI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiEI BsiWI BspEI BssHII
BssSI BstBI BstEII BstPI Bsu36I
CelII CfoI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I KasI KpnI KspI MaeII
MamI MluI MroI MspA1I NarI
NciI NdeI NheI NotI NruI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspI StuI SwaI Tth111I Van91I
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AccI 1503 GTATAC GT^[AC][GT]AC
AccII 1386 CGCG CG^CG
AflIII 962 ACATGT A^C[GA][CT]GT
Asp700I 937 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1232 CTCGAG C^[CT]CG[GA]G
BciVI 79 GGATAC GTATCC(6/5)
BclI 497 TGATCA T^GATCA
BglII 1443 AGATCT A^GATCT
BpmI 772 CTGGAG CTGGAG(16/14)
BsgI 1129 CTGCAC GTGCAG(16/14)
BsmAI 1177 GAGAC GTCTC(1/5)
BsmBI 1177 GAGACG CGTCTC(1/5)
BsmFI 675 GGGAC GGGAC(10/14)
BsmI 1003 GAATGC GAATGC(1/-1)
BsoBI 1232 CTCGAG C^[CT]CG[GA]G
Bsp1407I 1247 TGTACA T^GTACA
BspDI 1666 ATCGAT AT^CGAT
BspHI 1835 TCATGA T^CATGA
BspLU11I 962 ACATGT A^CATGT
BsrBI 1189 GAGCGG CCGCTC(-3/-3)
BsrDI 74 GCAATG GCAATG(2/0)
BsrFI 1037 GCCGGC [GA]^CCGG[CT]
BsrGI 1247 TGTACA T^GTACA
Bst1107I 1503 GTATAC GTA^TAC
BstAPI 1747 GCAAAATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1386 CGCG CG^CG
BstXI 1757 CCATCTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1443 AGATCT [GA]^GATC[CT]
BstZ17I 1503 GTATAC GTA^TAC
Cfr10I 1037 GCCGGC [GA]^CCGG[CT]
ClaI 1666 ATCGAT AT^CGAT
DrdI 1555 GACATTATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 234 CCATGG C^C[GA][CT]GG
EcoNI 645 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 234 CCATGG C^C[AT][AT]GG
EcoT22I 1005 ATGCAT ATGCA^T
FauI 975 GCGGG CCCGC(4/6)
FbaI 497 TGATCA T^GATCA
HapII 1038 CCGG C^CGG
HpaI 1225 GTTAAC GTT^AAC
HpaII 1038 CCGG C^CGG
MfeI 521 CAATTG C^AATTG
MflI 1443 AGATCT [GA]^GATC[CT]
MspI 1038 CCGG C^CGG
MunI 521 CAATTG C^AATTG
MvnI 1386 CGCG CG^CG
NaeI 1037 GCCGGC GCC^GGC
NcoI 234 CCATGG C^CATGG
NgoMIV 1037 GCCGGC G^CCGGC
NsiI 1005 ATGCAT ATGCA^T
PaeR7I 1232 CTCGAG C^TCGAG
PstI 1012 CTGCAG CTGCA^G
RcaI 1835 TCATGA T^CATGA
ScaI 868 AGTACT AGT^ACT
SphI 1007 GCATGC GCATG^C
SspBI 1247 TGTACA T^GTACA
StyI 234 CCATGG C^C[AT][AT]GG
TfiI 1404 GAATC G^A[AT]TC
XcmI 1079 CCAAGTTGAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1232 CTCGAG C^TCGAG
XhoII 1443 AGATCT [GA]^GATC[CT]
XmnI 937 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AspHI 37 GAGCTC G[AT]GC[AT]^C
802 GAGCTC
BalI 2 TGGCCA TGG^CCA
1536 TGGCCA
BanI 337 GGCACC G^G[CT][GA]CC
614 GGTGCC
BbsI 1707 GAAGAC GAAGAC(2/6)
1804 GAAGAC
BfaI 329 CTAG C^TAG
1616 CTAG
BpuAI 1707 GAAGAC GAAGAC(2/6)
1804 GAAGAC
BsiHKAI 37 GAGCTC G[AT]GC[AT]^C
802 GAGCTC
BspMI 902 ACCTGC ACCTGC(4/8)
912 GCAGGT
DraII 641 AGGACCT [GA]G^G[ACGT]CC[CT]
1625 AGGACCC
EaeI 2 TGGCCA [CT]^GGCC[GA]
1536 TGGCCA
EcoO109I 641 AGGACCT [GA]G^G[ACGT]CC[CT]
1625 AGGACCC
MaeI 329 CTAG C^TAG
1616 CTAG
MluNI 2 TGGCCA TGG^CCA
1536 TGGCCA
MscI 2 TGGCCA TGG^CCA
1536 TGGCCA
PleI 883 GACTC GAGTC(4/5)
1235 GAGTC
PpuMI 641 AGGACCT [GA]G^G[AT]CC[CT]
1625 AGGACCC
SacI 37 GAGCTC GAGCT^C
802 GAGCTC
SapI 799 GAAGAGC GCTCTTC(1/4)
1186 GAAGAGC
SexAI 1680 ACCAGGT A^CC[AT]GGT
1808 ACCTGGT
SfcI 176 CTACAG C^T[GA][CT]AG
1012 CTGCAG
Tsp45I 1048 GTCAC ^GT[GC]AC
1253 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanII BbvCI BbvI BmyI BsaJI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EarI EcoRII Fnu4HI FokI
HaeIII HincII HindII HindIII HinfI
HphI ItaI Ksp632I MaeIII MboI
MboII MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV NspI
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI TaqI Tru9I TseI Tsp509I
TspRI TthHB8I