Restriction map for
pFN21AE1492
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AgeI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
AspI BalI BamHI BbeI BbrPI
BbvCI BglI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspEI BspHI BsrBI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CpoI DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV FseI HaeII
HgaI Hin1I HpaI KasI KspI
MluI MluNI MroI MscI MspA1I
MvnI NarI NdeI NheI NotI
NruI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI StuI SwaI Tth111I XmaCI
XmaI
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# ONE CUT ENZYMES ( 62 enzymes) #
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Acc65I 798 GGTACC G^GTACC
AflII 301 CTTAAG C^TTAAG
Asp700I 2413 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 798 GGTACC G^GTACC
AvaI 344 CTCGAG C^[CT]CG[GA]G
AviII 456 TGCGCA TGC^GCA
AvrII 1429 CCTAGG C^CTAGG
BanI 798 GGTACC G^G[CT][GA]CC
BbsI 1320 GAAGAC GAAGAC(2/6)
BcgI 312 GCAACTTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 301 CTTAAG C^TTAAG
BglII 268 AGATCT A^GATCT
BlnI 1429 CCTAGG C^CTAGG
BlpI 2295 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2295 GCTGAGC GC^T[ACGT]AGC
BpuAI 1320 GAAGAC GAAGAC(2/6)
BsaAI 1667 CACGTA [CT]AC^GT[GA]
BseRI 1969 GAGGAG GAGGAG(10/8)
BsoBI 344 CTCGAG C^[CT]CG[GA]G
BspDI 1012 ATCGAT AT^CGAT
BsrDI 1886 CATTGC GCAATG(2/0)
BsrFI 2307 GCCGGC [GA]^CCGG[CT]
BssSI 433 CTCGTG CACGAG(-5/-1)
BstXI 2028 CCAGGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2295 GCTGAGC GC^T[ACGT]AGC
CfoI 457 GCGC GCG^C
Cfr10I 2307 GCCGGC [GA]^CCGG[CT]
ClaI 1012 ATCGAT AT^CGAT
DraII 2274 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 686 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 383 CCATGG C^C[GA][CT]GG
EaeI 2511 TGGCCG [CT]^GGCC[GA]
EcoO109I 2274 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 2041 GAATTC G^AATTC
EcoT22I 2063 ATGCAT ATGCA^T
FauI 481 GCGGG CCCGC(4/6)
FspI 456 TGCGCA TGC^GCA
HhaI 457 GCGC GCG^C
HinP1I 457 GCGC G^CGC
HincII 1651 GTTGAC GT[CT]^[GA]AC
HindII 1651 GTTGAC GT[CT]^[GA]AC
KpnI 798 GGTACC GGTAC^C
MaeII 1668 ACGT A^CGT
MfeI 1165 CAATTG C^AATTG
MunI 1165 CAATTG C^AATTG
NaeI 2307 GCCGGC GCC^GGC
NcoI 383 CCATGG C^CATGG
NgoMIV 2307 GCCGGC G^CCGGC
NsiI 2063 ATGCAT ATGCA^T
PaeR7I 344 CTCGAG C^TCGAG
PflMI 1566 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2274 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1133 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2377 GAGCTC GAGCT^C
SapI 964 GCTCTTC GCTCTTC(1/4)
SexAI 1385 ACCTGGT A^CC[AT]GGT
SphI 712 GCATGC GCATG^C
Van91I 1566 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 271 TCTAGA T^CTAGA
XcmI 561 CCAGTTTGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 344 CTCGAG C^TCGAG
XmnI 2413 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AciI 481 GCGG CCGC(-3/-1)
2479 GCGG
AflIII 650 ACATGT A^C[GA][CT]GT
1078 ACATGT
AhdI 673 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1981 GACGATGAGTC
AlwNI 1028 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
1567 CAGCCTCTG
AspEI 673 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1981 GACGATGAGTC
AspHI 2082 GTGCTC G[AT]GC[AT]^C
2377 GAGCTC
BanII 1866 GAGCCC G[GA]GC[CT]^C
2377 GAGCTC
BciVI 5 GGATAC GTATCC(6/5)
87 GGATAC
BclI 585 TGATCA T^GATCA
1710 TGATCA
BpmI 2471 CTCCAG CTGGAG(16/14)
1835 CTGGAG
BsaBI 786 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
910 GATGTTGATC
BsaI 1628 GAGACC GGTCTC(1/5)
413 GGTCTC
BsiHKAI 2082 GTGCTC G[AT]GC[AT]^C
2377 GAGCTC
BspLU11I 650 ACATGT A^CATGT
1078 ACATGT
BstYI 268 AGATCT [GA]^GATC[CT]
516 GGATCT
DraI 549 TTTAAA TTT^AAA
2398 TTTAAA
Eam1105I 673 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1981 GACGATGAGTC
FbaI 585 TGATCA T^GATCA
1710 TGATCA
MamI 786 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
910 GATGTTGATC
MflI 268 AGATCT [GA]^GATC[CT]
516 GGATCT
PstI 2050 CTGCAG CTGCA^G
2237 CTGCAG
ScaI 1183 AGTACT AGT^ACT
1210 AGTACT
SfcI 2050 CTGCAG C^T[GA][CT]AG
2237 CTGCAG
SspI 2122 AATATT AAT^ATT
2529 AATATT
TaqI 345 TCGA T^CGA
1013 TCGA
TthHB8I 345 TCGA T^CGA
1013 TCGA
XhoII 268 AGATCT [GA]^GATC[CT]
516 GGATCT
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AvaII BbvI BcnI BfaI BmyI
BsaJI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI