Restriction map for
pFN21AE4364
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AvaI
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BcgI BciVI BclI
BfaI BfrI BlnI BpmI BpuAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BsmI BsoBI Bsp1407I BspEI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstXI BstZ17I Cfr10I CpoI DraI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MamI
MfeI MluI MroI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccI 157 GTAGAC GT^[AC][GT]AC
AcyI 637 GACGCC G[GA]^CG[CT]C
AlwNI 823 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 160 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 363 GGACC G^G[AT]CC
BbvCI 66 CCTCAGC CCTCAGC(-5/-2)
BglII 785 AGATCT A^GATCT
BlpI 378 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 378 GCTCAGC GC^T[ACGT]AGC
BsaHI 637 GACGCC G[GA]^CG[CT]C
BsgI 1048 GTGCAG GTGCAG(16/14)
BsmBI 353 GAGACG CGTCTC(1/5)
BspDI 562 ATCGAT AT^CGAT
BspHI 206 TCATGA T^CATGA
BspMI 844 ACCTGC ACCTGC(4/8)
BstEII 193 GGTCACC G^GT[ACGT]ACC
BstPI 193 GGTCACC G^GT[ACGT]ACC
CelII 378 GCTCAGC GC^T[ACGT]AGC
ClaI 562 ATCGAT AT^CGAT
EcoO65I 193 GGTCACC G^GT[ACGT]ACC
HaeII 449 AGCGCC [GA]GCGC^[CT]
Hin1I 637 GACGCC G[GA]^CG[CT]C
MseI 791 TTAA T^TAA
NcoI 716 CCATGG C^CATGG
NspI 885 GCATGT [GA]CATG^[CT]
PflFI 160 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 822 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 206 TCATGA T^CATGA
SapI 528 GAAGAGC GCTCTTC(1/4)
ScaI 515 AGTACT AGT^ACT
SmlI 343 CTCAAG C^T[CT][GA]AG
Tru9I 791 TTAA T^TAA
Tsp45I 194 GTCAC ^GT[GC]AC
Tsp509I 37 AATT ^AATT
Tth111I 160 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 822 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AccII 388 CGCG CG^CG
625 CGCG
AflIII 419 ACACGT A^C[GA][CT]GT
1004 ACGTGT
AocI 46 CCTGAGG CC^T[ACGT]AGG
611 CCTCAGG
AspHI 376 GTGCTC G[AT]GC[AT]^C
838 GTGCTC
BalI 89 TGGCCA TGG^CCA
801 TGGCCA
BanII 990 GGGCTC G[GA]GC[CT]^C
1026 GGGCTC
BcnI 453 CCGGG CC^[GC]GG
705 CCGGG
BglI 287 GCCACGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
491 GCCTGACGGGC
BsaAI 373 TACGTG [CT]AC^GT[GA]
835 TACGTG
BsiHKAI 376 GTGCTC G[AT]GC[AT]^C
838 GTGCTC
BsmAI 136 GAGAC GTCTC(1/5)
353 GAGAC
BsmFI 128 GGGAC GGGAC(10/14)
594 GTCCC
BstUI 388 CGCG CG^CG
625 CGCG
BstYI 785 AGATCT [GA]^GATC[CT]
929 AGATCC
Bsu36I 46 CCTGAGG CC^T[ACGT]AGG
611 CCTCAGG
CfoI 450 GCGC GCG^C
898 GCGC
EarI 528 GAAGAG CTCTTC(1/4)
954 GAAGAG
Eco81I 46 CCTGAGG CC^T[ACGT]AGG
611 CCTCAGG
EcoT14I 92 CCAAGG C^C[AT][AT]GG
716 CCATGG
HapII 453 CCGG C^CGG
705 CCGG
HhaI 450 GCGC GCG^C
898 GCGC
HinP1I 450 GCGC G^CGC
898 GCGC
HinfI 138 GACTC G^A[ACGT]TC
238 GAGTC
HpaII 453 CCGG C^CGG
705 CCGG
Ksp632I 528 GAAGAG CTCTTC(1/4)
954 GAAGAG
MaeIII 11 GTAAC ^GT[ACGT]AC
194 GTCAC
MflI 785 AGATCT [GA]^GATC[CT]
929 AGATCC
MluNI 89 TGGCCA TGG^CCA
801 TGGCCA
MscI 89 TGGCCA TGG^CCA
801 TGGCCA
MslI 207 CATGACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
711 CATCTCCATG
MspI 453 CCGG C^CGG
705 CCGG
MvnI 388 CGCG CG^CG
625 CGCG
NciI 453 CCGGG CC^[GC]GG
705 CCGGG
NlaIV 363 GGACCC GG[ACGT]^[ACGT]CC
818 GGAACC
PleI 138 GACTC GAGTC(4/5)
238 GAGTC
PstI 769 CTGCAG CTGCA^G
949 CTGCAG
PvuII 980 CAGCTG CAG^CTG
1051 CAGCTG
SfaNI 407 GCATC GCATC(5/9)
647 GCATC
StyI 92 CCAAGG C^C[AT][AT]GG
716 CCATGG
XhoII 785 AGATCT [GA]^GATC[CT]
929 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BmyI BsaJI BseRI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DsaI
EaeI EcoRII FauI Fnu4HI FokI
HaeIII HgaI HphI ItaI MaeII
MboI MboII MnlI MspA1I MvaI
MwoI NdeII NlaIII RsaI Sau3AI
Sau96I ScrFI SfcI TaqI TseI
TspRI TthHB8I