Restriction map for
pFN21AE3938
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# NO CUT ENZYMES ( 162 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BamHI BanI
BbeI BbrPI BbsI BciVI BclI
BfaI BfrI BglI BglII BlnI
BpuAI BsaBI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmFI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstXI BstYI BstZ17I Bsu36I
ClaI DraI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MamI
MfeI MflI MluI MroI MseI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsaI SacI
SacII SalI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tsp509I Tth111I XbaI XcmI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcyI 36 GGCGTC G[GA]^CG[CT]C
AlwNI 67 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 185 CCCGGG C^[CT]CG[GA]G
BbvCI 534 CCTCAGC CCTCAGC(-5/-2)
BcgI 14 GCAAGGTGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 403 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 403 GCTCAGC GC^T[ACGT]AGC
BsaAI 127 TACGTG [CT]AC^GT[GA]
BsaHI 36 GGCGTC G[GA]^CG[CT]C
BsmBI 109 GAGACG CGTCTC(1/5)
BsoBI 185 CCCGGG C^[CT]CG[GA]G
BspMI 165 GCAGGT ACCTGC(4/8)
BsrFI 458 ACCGGC [GA]^CCGG[CT]
BsrI 410 ACTGG ACTGG(1/-1)
BstEII 336 GGTCACC G^GT[ACGT]ACC
BstF5I 567 GGATG GGATG(2/0)
BstPI 336 GGTCACC G^GT[ACGT]ACC
Cac8I 493 GCCAGC GC[ACGT]^[ACGT]GC
CelII 403 GCTCAGC GC^T[ACGT]AGC
CfoI 462 GCGC GCG^C
Cfr10I 458 ACCGGC [GA]^CCGG[CT]
CpoI 455 CGGACCG CG^G[AT]CCG
DpnII 105 GATC ^GATC
EarI 6 GAAGAG CTCTTC(1/4)
EcoO65I 336 GGTCACC G^GT[ACGT]ACC
FokI 567 GGATG GGATG(9/13)
HaeII 461 GGCGCT [GA]GCGC^[CT]
HhaI 462 GCGC GCG^C
Hin1I 36 GGCGTC G[GA]^CG[CT]C
HinP1I 462 GCGC G^CGC
HinfI 274 GAGTC G^A[ACGT]TC
Ksp632I 6 GAAGAG CTCTTC(1/4)
MboI 105 GATC ^GATC
NdeII 105 GATC ^GATC
NlaIII 85 CATG CATG^
PflMI 84 CCATGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 274 GAGTC GAGTC(4/5)
RsrII 455 CGGACCG CG^G[AT]CCG
SapI 6 GAAGAGC GCTCTTC(1/4)
Sau3AI 105 GATC ^GATC
SfcI 255 CTATAG C^T[GA][CT]AG
SmaI 185 CCCGGG CCC^GGG
SmlI 298 CTCAAG C^T[CT][GA]AG
TaqI 107 TCGA T^CGA
TthHB8I 107 TCGA T^CGA
Van91I 84 CCATGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 185 CCCGGG C^CCGGG
XmaI 185 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccI 388 GTAGAC GT^[AC][GT]AC
484 GTCTAC
AvaII 348 GGTCC G^G[AT]CC
456 GGACC
BalI 30 TGGCCA TGG^CCA
491 TGGCCA
BpmI 61 CTGGAG CTGGAG(16/14)
472 CTGGAG
BsmAI 109 GAGAC GTCTC(1/5)
142 GAGAC
DdeI 404 CTCAG C^T[ACGT]AG
535 CTCAG
EaeI 30 TGGCCA [CT]^GGCC[GA]
491 TGGCCA
HphI 429 GGTGA GGTGA(8/7)
338 TCACC
MaeII 128 ACGT A^CGT
245 ACGT
MaeIII 337 GTCAC ^GT[ACGT]AC
365 GTGAC
MboII 6 GAAGA GAAGA(8/7)
543 GAAGA
MluNI 30 TGGCCA TGG^CCA
491 TGGCCA
MscI 30 TGGCCA TGG^CCA
491 TGGCCA
MslI 123 CATCTACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
339 CACCATCGTG
MspA1I 379 CAGCGG C[AC]G^C[GT]G
555 CAGCGG
Tsp45I 337 GTCAC ^GT[GC]AC
365 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AluI BanII BbvI BcnI
BmyI BsaJI BsiYI BslI Bsp1286I
BssKI BstNI Cfr13I EcoRII EcoT14I
Fnu4HI HaeIII HapII HgaI HpaII
ItaI MnlI MspI MvaI MwoI
NciI NlaIV Sau96I ScrFI StyI
TseI TspRI