Restriction map for
pFN21AE4373
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII AccIII AclI AcyI AflII
AflIII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I AspEI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BlnI
BlpI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr10I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HaeII HapII
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII KasI MfeI
MluI MluNI MroI MscI MspI
MunI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmeI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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Acc65I 1067 GGTACC G^GTACC
AccI 275 GTAGAC GT^[AC][GT]AC
AccII 1105 CGCG CG^CG
AcsI 408 GAATTT [GA]^AATT[CT]
ApaI 895 GGGCCC GGGCC^C
ApoI 408 GAATTT [GA]^AATT[CT]
Asp718I 1067 GGTACC G^GTACC
BanI 1067 GGTACC G^G[CT][GA]CC
BfaI 692 CTAG C^TAG
BglII 490 AGATCT A^GATCT
BsaBI 757 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 494 CTGCAC GTGCAG(16/14)
BstUI 1105 CGCG CG^CG
BstXI 233 CCATCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 490 AGATCT [GA]^GATC[CT]
DraIII 872 CACGTAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1125 TGGCCG [CT]^GGCC[GA]
FauI 900 CCCGC CCCGC(4/6)
HgaI 181 GACGC GACGC(5/10)
HphI 161 TCACC GGTGA(8/7)
KpnI 1067 GGTACC GGTAC^C
KspI 1104 CCGCGG CCGC^GG
MaeI 692 CTAG C^TAG
MamI 757 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 490 AGATCT [GA]^GATC[CT]
MvnI 1105 CGCG CG^CG
NdeI 851 CATATG CA^TATG
SacI 539 GAGCTC GAGCT^C
SacII 1104 CCGCGG CCGC^GG
ScaI 108 AGTACT AGT^ACT
SfaNI 190 GATGC GCATC(5/9)
Tsp509I 409 AATT ^AATT
TspRI 522 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 490 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
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AfaI 109 GTAC GT^AC
1068 GTAC
BanII 539 GAGCTC G[GA]GC[CT]^C
895 GGGCCC
BsmAI 200 GAGAC GTCTC(1/5)
971 GAGAC
DsaI 1104 CCGCGG C^C[GA][CT]GG
1128 CCGTGG
EcoNI 313 CCTCCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
886 CCTACACGAGG
HinfI 847 GATTC G^A[ACGT]TC
1084 GATTC
MseI 44 TTAA T^TAA
413 TTAA
MspA1I 592 CCGCTG C[AC]G^C[GT]G
1104 CCGCGG
PpuMI 360 GGGACCT [GA]G^G[AT]CC[CT]
474 GGGTCCT
RsaI 109 GTAC GT^AC
1068 GTAC
SapI 536 GAAGAGC GCTCTTC(1/4)
27 GCTCTTC
SfcI 840 CTACAG C^T[GA][CT]AG
1089 CTACAG
SmlI 981 CTCAAG C^T[CT][GA]AG
1005 CTCAAG
TaqI 582 TCGA T^CGA
969 TCGA
TfiI 847 GATTC G^A[AT]TC
1084 GATTC
Tru9I 44 TTAA T^TAA
413 TTAA
TthHB8I 582 TCGA T^CGA
969 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
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AciI AluI AspHI AvaII BbrPI
BbvI BmyI BsaAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BssSI BstF5I BstNI
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII Fnu4HI FokI HaeIII
ItaI Ksp632I MaeII MaeIII MboI
MboII MnlI MslI MvaI MwoI
NdeII NlaIII NlaIV PflMI PmaCI
PmlI Sau3AI Sau96I ScrFI TseI
Tsp45I Van91I