Restriction map for
pFN21AE5122
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# NO CUT ENZYMES ( 182 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BsiEI BsiWI
BsiYI BslI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrFI BsrGI BsrI BssHII Bst1107I
BstAPI BstBI BstEII BstNI BstPI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRII EcoRV EcoT22I
FauI FbaI FseI FspI HaeIII
HincII HindII HindIII HinfI HpaI
HphI KasI KpnI KspI MaeII
MamI MfeI MflI MluNI MroI
MscI MseI MslI MspA1I MunI
MvaI MwoI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tru9I Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AcyI 6 GGCGTC G[GA]^CG[CT]C
AflIII 83 ACGCGT A^C[GA][CT]GT
AluI 52 AGCT AG^CT
AlwNI 64 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 51 GAGCTC G[AT]GC[AT]^C
AvaI 171 CTCGGG C^[CT]CG[GA]G
AvaII 146 GGACC G^G[AT]CC
BanII 51 GAGCTC G[GA]GC[CT]^C
BcnI 110 CCGGG CC^[GC]GG
BfaI 205 CTAG C^TAG
BmyI 51 GAGCTC G[AGT]GC[ACT]^C
BsaHI 6 GGCGTC G[GA]^CG[CT]C
BseRI 136 CTCCTC GAGGAG(10/8)
BsgI 310 CTGCAC GTGCAG(16/14)
BsiHKAI 51 GAGCTC G[AT]GC[AT]^C
BsmAI 134 GTCTC GTCTC(1/5)
BsmFI 145 GGGAC GGGAC(10/14)
BsoBI 171 CTCGGG C^[CT]CG[GA]G
Bsp1286I 51 GAGCTC G[AGT]GC[ACT]^C
BspHI 323 TCATGA T^CATGA
BspMI 89 ACCTGC ACCTGC(4/8)
BssKI 110 CCGGG ^CC[ACGT]GG
BssSI 139 CTCGTG CACGAG(-5/-1)
CfoI 115 GCGC GCG^C
Cfr13I 146 GGACC G^G[ACGT]CC
DpnII 228 GATC ^GATC
DraII 145 GGGACCT [GA]G^G[ACGT]CC[CT]
EarI 40 GAAGAG CTCTTC(1/4)
EcoO109I 145 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 255 CCAAGG C^C[AT][AT]GG
HaeII 114 GGCGCT [GA]GCGC^[CT]
HapII 110 CCGG C^CGG
HhaI 115 GCGC GCG^C
Hin1I 6 GGCGTC G[GA]^CG[CT]C
HinP1I 115 GCGC G^CGC
HpaII 110 CCGG C^CGG
Ksp632I 40 GAAGAG CTCTTC(1/4)
MaeI 205 CTAG C^TAG
MaeIII 128 GTTAC ^GT[ACGT]AC
MboI 228 GATC ^GATC
MboII 40 GAAGA GAAGA(8/7)
MluI 83 ACGCGT A^CGCGT
MnlI 138 CCTC CCTC(7/6)
MspI 110 CCGG C^CGG
NciI 110 CCGGG CC^[GC]GG
NdeII 228 GATC ^GATC
NlaIII 324 CATG CATG^
NlaIV 145 GGGACC GG[ACGT]^[ACGT]CC
PpuMI 145 GGGACCT [GA]G^G[AT]CC[CT]
PstI 118 CTGCAG CTGCA^G
RcaI 323 TCATGA T^CATGA
SacI 51 GAGCTC GAGCT^C
Sau3AI 228 GATC ^GATC
Sau96I 146 GGACC G^G[ACGT]CC
ScaI 44 AGTACT AGT^ACT
ScrFI 110 CCGGG CC^[ACGT]GG
SfaNI 260 GCATC GCATC(5/9)
SfcI 118 CTGCAG C^T[GA][CT]AG
SmlI 48 CTTGAG C^T[CT][GA]AG
StyI 255 CCAAGG C^C[AT][AT]GG
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# TWO CUT ENZYMES ( 14 enzymes) #
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AccII 29 CGCG CG^CG
84 CGCG
AfaI 45 GTAC GT^AC
87 GTAC
BbvI 63 GCAGC GCAGC(8/12)
117 GCTGC
BsaJI 110 CCGGGG C^C[ACGT][ACGT]GG
255 CCAAGG
BstF5I 201 CATCC GGATG(2/0)
306 CATCC
BstUI 29 CGCG CG^CG
84 CGCG
Cac8I 273 GCGAGC GC[ACGT]^[ACGT]GC
298 GCCAGC
DdeI 36 CTTAG C^T[ACGT]AG
217 CTGAG
Fnu4HI 63 GCAGC GC^[ACGT]GC
117 GCTGC
FokI 201 CATCC GGATG(9/13)
306 CATCC
ItaI 63 GCAGC GC^[ACGT]GC
117 GCTGC
MvnI 29 CGCG CG^CG
84 CGCG
RsaI 45 GTAC GT^AC
87 GTAC
TseI 63 GCAGC G^C[AT]GC
117 GCTGC
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# 3 OR MORE CUT ENZYMES ( 2 enzymes) #
#########################################
AciI HgaI