##################################
# NO CUT ENZYMES (  183 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       AvrII       BanII       BbeI        
BbsI        BbvCI       BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaBI       
BsaHI       BsaI        BsaJI       BseAI       BseRI       
BsiEI       BsiHKAI     BsiWI       BsiYI       BslI        
BsmAI       BsmBI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      Cfr13I      ClaI        CpoI        DdeI        
DraI        DraII       DraIII      DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       HhaI        Hin1I       
HinP1I      HindIII     KasI        KpnI        KspI        
MaeI        MamI        MfeI        MluI        MroI        
MslI        MspA1I      MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NlaIII      NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmeI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        Sau96I      SbfI        ScaI        SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AcsI          105  GAATTT               [GA]^AATT[CT]
AlwNI         299  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          105  GAATTT               [GA]^AATT[CT]
BalI          362  TGGCCA               TGG^CCA
BamHI         356  GGATCC               G^GATCC
BanI          345  GGCACC               G^G[CT][GA]CC
BbrPI         424  CACGTG               CAC^GTG
BmyI           81  GTGCCC               G[AGT]GC[ACT]^C
BpmI          467  CTCCAG               CTGGAG(16/14)
BsaAI         424  CACGTG               [CT]AC^GT[GA]
BsaWI          93  TCCGGT               [AT]^CCGG[AT]
BsgI           97  GTGCAG               GTGCAG(16/14)
BsmFI          57  GGGAC                GGGAC(10/14)
Bsp1286I       81  GTGCCC               G[AGT]GC[ACT]^C
BspMI         403  ACCTGC               ACCTGC(4/8)
BssSI         388  CTCGTG               CACGAG(-5/-1)
EaeI          362  TGGCCA               [CT]^GGCC[GA]
EarI           40  CTCTTC               CTCTTC(1/4)
HapII          94  CCGG                 C^CGG
HgaI          176  GACGC                GACGC(5/10)
HincII        289  GTTAAC               GT[CT]^[GA]AC
HindII        289  GTTAAC               GT[CT]^[GA]AC
HpaI          289  GTTAAC               GTT^AAC
HpaII          94  CCGG                 C^CGG
Ksp632I        40  CTCTTC               CTCTTC(1/4)
MaeIII        535  GTCAC                ^GT[ACGT]AC
MluNI         362  TGGCCA               TGG^CCA
MscI          362  TGGCCA               TGG^CCA
MspI           94  CCGG                 C^CGG
PleI          481  GACTC                GAGTC(4/5)
PmaCI         424  CACGTG               CAC^GTG
PmlI          424  CACGTG               CAC^GTG
SexAI         542  ACCAGGT              A^CC[AT]GGT
TaqI          269  TCGA                 T^CGA
TfiI          261  GAATC                G^A[AT]TC
Tsp45I        535  GTCAC                ^GT[GC]AC
TthHB8I       269  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

AciI           26  CCGC                 CCGC(-3/-1)
               49  GCGG                
BstF5I        183  CATCC                GGATG(2/0)
              411  CATCC               
Cac8I          34  GCTGGC               GC[ACGT]^[ACGT]GC
              303  GCCTGC              
FokI          183  CATCC                GGATG(9/13)
              411  CATCC               
HinfI         261  GAATC                G^A[ACGT]TC
              481  GACTC               
MaeII         113  ACGT                 A^CGT
              425  ACGT                
NlaIV         345  GGCACC               GG[ACGT]^[ACGT]CC
              356  GGATCC              
Tsp509I       106  AATT                 ^AATT
              144  AATT                
TspRI          29  CACTG                CA[GC]TG[ACGT][ACGT]^
              476  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   29 enzymes) #
#########################################

AfaI        AluI        AlwI        BbvI        BsrI        
BssKI       BstNI       BstYI       DpnII       EcoRII      
Fnu4HI      HaeIII      HphI        ItaI        MboI        
MboII       MflI        MnlI        MseI        MvaI        
MwoI        NdeII       RsaI        Sau3AI      ScrFI       
SfaNI       Tru9I       TseI        XhoII