Restriction map for
pFN21AE6223
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AflIII AgeI Alw44I Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I AspHI AspI AvrII
BamHI BanII BbeI BbrPI BbvCI
BciVI BclI BfaI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstXI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV FauI FbaI FseI
HaeII Hin1I HincII HindII HindIII
HpaI KasI KspI MaeI MaeII
MamI MfeI MluI MroI MslI
MspA1I MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NspI PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI SspBI SspI SwaI
TfiI Tsp509I Tth111I XbaI XcmI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 251 GGTACC G^GTACC
AflII 120 CTTAAG C^TTAAG
AhdI 22 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 256 CCTCAGG CC^T[ACGT]AGG
Asp718I 251 GGTACC G^GTACC
AspEI 22 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 439 CTCGAG C^[CT]CG[GA]G
AviII 89 TGCGCA TGC^GCA
BbsI 550 GTCTTC GAAGAC(2/6)
BcgI 126 CGATGCCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 333 CCGGG CC^[GC]GG
BfrI 120 CTTAAG C^TTAAG
BglI 403 GCCACCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 550 GTCTTC GAAGAC(2/6)
BsoBI 439 CTCGAG C^[CT]CG[GA]G
BstBI 553 TTCGAA TT^CGAA
BstYI 99 GGATCT [GA]^GATC[CT]
Bsu36I 256 CCTCAGG CC^T[ACGT]AGG
DsaI 530 CCGTGG C^C[GA][CT]GG
Eam1105I 22 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 616 CTCTTC CTCTTC(1/4)
Eco81I 256 CCTCAGG CC^T[ACGT]AGG
EcoT14I 650 CCAAGG C^C[AT][AT]GG
EcoT22I 523 ATGCAT ATGCA^T
FspI 89 TGCGCA TGC^GCA
HgaI 222 GACGC GACGC(5/10)
HinfI 312 GACTC G^A[ACGT]TC
KpnI 251 GGTACC GGTAC^C
Ksp632I 616 CTCTTC CTCTTC(1/4)
MaeIII 424 GTGAC ^GT[ACGT]AC
MflI 99 GGATCT [GA]^GATC[CT]
NciI 333 CCGGG CC^[GC]GG
NsiI 523 ATGCAT ATGCA^T
NspV 553 TTCGAA TT^CGAA
PaeR7I 439 CTCGAG C^TCGAG
PflMI 390 CCAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 312 GACTC GAGTC(4/5)
SbfI 264 CCTGCAGG CCTGCA^GG
SfuI 553 TTCGAA TT^CGAA
Sse8387I 264 CCTGCAGG CCTGCA^GG
StuI 464 AGGCCT AGG^CCT
StyI 650 CCAAGG C^C[AT][AT]GG
Tsp45I 424 GTGAC ^GT[GC]AC
Van91I 390 CCAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 439 CTCGAG C^TCGAG
XhoII 99 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
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AlwI 714 GATCC GGATC(4/5)
99 GGATC
BanI 159 GGTGCC G^G[CT][GA]CC
251 GGTACC
BmyI 134 GTGCCC G[AGT]GC[ACT]^C
275 GTGCCC
BpmI 454 CTCCAG CTGGAG(16/14)
7 CTGGAG
Bsp1286I 134 GTGCCC G[AGT]GC[ACT]^C
275 GTGCCC
DdeI 257 CTCAG C^T[ACGT]AG
492 CTTAG
DpnII 100 GATC ^GATC
714 GATC
EcoNI 150 CCTCAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
615 CCTCTTCCAGG
HapII 333 CCGG C^CGG
717 CCGG
HpaII 333 CCGG C^CGG
717 CCGG
MboI 100 GATC ^GATC
714 GATC
MseI 107 TTAA T^TAA
121 TTAA
MspI 333 CCGG C^CGG
717 CCGG
NdeII 100 GATC ^GATC
714 GATC
Sau3AI 100 GATC ^GATC
714 GATC
Tru9I 107 TTAA T^TAA
121 TTAA
TspRI 395 CACTG CA[GC]TG[ACGT][ACGT]^
602 CAGTG
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# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AvaII BalI BbvI BsaJI BsiYI
BslI BsmFI BspMI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DraII EaeI EcoO109I EcoRII
Fnu4HI FokI HaeIII HhaI HinP1I
HphI ItaI MboII MluNI MnlI
MscI MvaI MvnI MwoI NlaIII
NlaIV PpuMI PstI RsaI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TseI TthHB8I