Restriction map for
pFN21AB8859
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# NO CUT ENZYMES ( 192 enzymes) #
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Acc65I AccI AccII AccIII AciI
AclI AcsI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BcnI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DdeI DraI DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FauI FseI
FspI HaeII HaeIII HapII HhaI
HinP1I HincII HindII HindIII HpaI
HpaII HphI KasI KpnI Ksp632I
KspI MamI MboII MfeI MflI
MluI MluNI MroI MscI MseI
MspA1I MspI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NlaIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
TfiI Tru9I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 37 GACGTC GACGT^C
AfaI 261 GTAC GT^AC
AlwNI 76 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 94 GAGCTC G[AT]GC[AT]^C
AvaII 177 GGACC G^G[AT]CC
BanII 94 GAGCTC G[GA]GC[CT]^C
BclI 29 TGATCA T^GATCA
BfaI 10 CTAG C^TAG
BmyI 94 GAGCTC G[AGT]GC[ACT]^C
BsiHKAI 94 GAGCTC G[AT]GC[AT]^C
BsmAI 13 GAGAC GTCTC(1/5)
Bsp1286I 94 GAGCTC G[AGT]GC[ACT]^C
BstF5I 153 GGATG GGATG(2/0)
Cac8I 71 GCACGC GC[ACGT]^[ACGT]GC
Cfr13I 177 GGACC G^G[ACGT]CC
DpnII 30 GATC ^GATC
DraII 176 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoNI 81 CCTGACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 176 AGGACCT [GA]G^G[ACGT]CC[CT]
FbaI 29 TGATCA T^GATCA
FokI 153 GGATG GGATG(9/13)
HgaI 184 GACGC GACGC(5/10)
HinfI 278 GACTC G^A[ACGT]TC
MaeI 10 CTAG C^TAG
MaeIII 254 GTCAC ^GT[ACGT]AC
MboI 30 GATC ^GATC
MnlI 64 GAGG CCTC(7/6)
MslI 21 CATGGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 20 CCATGG C^CATGG
NdeII 30 GATC ^GATC
PleI 278 GACTC GAGTC(4/5)
PpuMI 176 AGGACCT [GA]G^G[AT]CC[CT]
PstI 133 CTGCAG CTGCA^G
RsaI 261 GTAC GT^AC
SacI 94 GAGCTC GAGCT^C
Sau3AI 30 GATC ^GATC
Sau96I 177 GGACC G^G[ACGT]CC
ScaI 260 AGTACT AGT^ACT
SfcI 133 CTGCAG C^T[GA][CT]AG
Tsp45I 254 GTCAC ^GT[GC]AC
Tsp509I 167 AATT ^AATT
TspRI 213 CAGTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 13 enzymes) #
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AcyI 37 GACGTC G[GA]^CG[CT]C
184 GACGCC
AluI 95 AGCT AG^CT
119 AGCT
BsaHI 37 GACGTC G[GA]^CG[CT]C
184 GACGCC
BsiYI 48 CCGATATTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
81 CCTGACCAAGG
BslI 48 CCGATATTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
81 CCTGACCAAGG
DsaI 20 CCATGG C^C[GA][CT]GG
158 CCGTGG
EcoT14I 20 CCATGG C^C[AT][AT]GG
86 CCAAGG
Hin1I 37 GACGTC G[GA]^CG[CT]C
184 GACGCC
MaeII 38 ACGT A^CGT
244 ACGT
NlaIII 21 CATG CATG^
27 CATG
SfaNI 154 GATGC GCATC(5/9)
196 GATGC
SmlI 97 CTCAAG C^T[CT][GA]AG
172 CTCAAG
StyI 20 CCATGG C^C[AT][AT]GG
86 CCAAGG
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# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
BbvI BsaJI BssKI BstNI EcoRII
Fnu4HI ItaI MvaI MwoI ScrFI
TseI