Restriction map for
pFN21AE2584
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AvaI AviII AvrII
BalI BanI BbeI BbrPI BcgI
BciVI BclI BfrI BglI BglII
BlnI BpmI BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoT22I FbaI FseI
FspI HgaI Hin1I HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MseI MunI
MvnI NarI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsaI RsrII SacII
SapI SbfI ScaI SexAI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 79 GTCGAC GT^[AC][GT]AC
AcsI 30 GAATTC [GA]^AATT[CT]
ApoI 30 GAATTC [GA]^AATT[CT]
AspI 360 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 603 GGATCC G^GATCC
BcnI 98 CCCGG CC^[GC]GG
BfaI 308 CTAG C^TAG
BlpI 151 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 151 GCTGAGC GC^T[ACGT]AGC
BsaI 303 GGTCTC GGTCTC(1/5)
BsgI 298 GTGCAG GTGCAG(16/14)
BsmFI 514 GGGAC GGGAC(10/14)
BspMI 300 GCAGGT ACCTGC(4/8)
BsrFI 10 GCCGGC [GA]^CCGG[CT]
BsrI 363 CCAGT ACTGG(1/-1)
BstAPI 291 GCATGACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 69 GGATG GGATG(2/0)
BstYI 603 GGATCC [GA]^GATC[CT]
CelII 151 GCTGAGC GC^T[ACGT]AGC
Cfr10I 10 GCCGGC [GA]^CCGG[CT]
DpnII 604 GATC ^GATC
DraII 194 AGGTCCT [GA]G^G[ACGT]CC[CT]
EaeI 12 CGGCCA [CT]^GGCC[GA]
EarI 169 GAAGAG CTCTTC(1/4)
EcoNI 37 CCTAACAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 194 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 30 GAATTC G^AATTC
EcoRV 19 GATATC GAT^ATC
FokI 69 GGATG GGATG(9/13)
HaeII 310 AGCGCC [GA]GCGC^[CT]
HincII 79 GTCGAC GT[CT]^[GA]AC
HindII 79 GTCGAC GT[CT]^[GA]AC
HindIII 460 AAGCTT A^AGCTT
Ksp632I 169 GAAGAG CTCTTC(1/4)
MaeI 308 CTAG C^TAG
MaeII 296 ACGT A^CGT
MboI 604 GATC ^GATC
MflI 603 GGATCC [GA]^GATC[CT]
MslI 84 CACAGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI 291 GCATGACGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI 10 GCCGGC GCC^GGC
NciI 98 CCCGG CC^[GC]GG
NdeII 604 GATC ^GATC
NgoMIV 10 GCCGGC G^CCGGC
PflFI 360 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 194 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 422 CAGCTG CAG^CTG
SalI 79 GTCGAC G^TCGAC
Sau3AI 604 GATC ^GATC
SmlI 229 CTCAAG C^T[CT][GA]AG
TaqI 80 TCGA T^CGA
Tth111I 360 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 80 TCGA T^CGA
XhoII 603 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwI 604 GATCC GGATC(4/5)
603 GGATC
AspHI 142 GAGCTC G[AT]GC[AT]^C
226 GAGCTC
BbsI 381 GAAGAC GAAGAC(2/6)
414 GAAGAC
BbvCI 572 CCTCAGC CCTCAGC(-5/-2)
130 GCTGAGG
BpuAI 381 GAAGAC GAAGAC(2/6)
414 GAAGAC
BsiHKAI 142 GAGCTC G[AT]GC[AT]^C
226 GAGCTC
BsmAI 467 GAGAC GTCTC(1/5)
304 GTCTC
Cac8I 10 GCCGGC GC[ACGT]^[ACGT]GC
287 GCTGGC
CfoI 189 GCGC GCG^C
311 GCGC
EcoT14I 161 CCAAGG C^C[AT][AT]GG
206 CCAAGG
FauI 607 CCCGC CCCGC(4/6)
577 GCGGG
HaeIII 13 GGCC GG^CC
244 GGCC
HapII 11 CCGG C^CGG
99 CCGG
HhaI 189 GCGC GCG^C
311 GCGC
HinP1I 189 GCGC G^CGC
311 GCGC
HpaII 11 CCGG C^CGG
99 CCGG
HphI 538 GGTGA GGTGA(8/7)
586 GGTGA
MspA1I 422 CAGCTG C[AC]G^C[GT]G
575 CAGCGG
MspI 11 CCGG C^CGG
99 CCGG
NlaIV 514 GGGACC GG[ACGT]^[ACGT]CC
603 GGATCC
PflMI 192 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
281 CCAGGAGCTGG
SacI 142 GAGCTC GAGCT^C
226 GAGCTC
StyI 161 CCAAGG C^C[AT][AT]GG
206 CCAAGG
Tsp509I 31 AATT ^AATT
177 AATT
Van91I 192 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
281 CCAGGAGCTGG
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AluI AlwNI AvaII BanII
BbvI BmyI BsaJI BseRI BsiYI
BslI Bsp1286I BssKI BstNI Cfr13I
DdeI DrdI EcoRII Fnu4HI HinfI
ItaI MaeIII MboII MnlI MvaI
NlaIII PleI Sau96I ScrFI TseI
Tsp45I TspRI