Restriction map for
pFN21AE6250
##################################
# NO CUT ENZYMES ( 154 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaBI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MflI MluNI MroI MscI
MseI MslI MspA1I MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Tth111I
XbaI XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AatII 412 GACGTC GACGT^C
AflIII 653 ACGCGT A^C[GA][CT]GT
ApaI 8 GGGCCC GGGCC^C
BpmI 425 CTCCAG CTGGAG(16/14)
BsaAI 559 TACGTG [CT]AC^GT[GA]
BsaI 201 GGTCTC GGTCTC(1/5)
BseRI 603 GAGGAG GAGGAG(10/8)
BsgI 172 CTGCAC GTGCAG(16/14)
BsrDI 691 GCAATG GCAATG(2/0)
BstEII 165 GGTGACC G^GT[ACGT]ACC
BstPI 165 GGTGACC G^GT[ACGT]ACC
BstXI 740 CCAAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 133 GACAGGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 144 CCATGG C^C[GA][CT]GG
EcoO65I 165 GGTGACC G^GT[ACGT]ACC
FauI 665 GCGGG CCCGC(4/6)
HaeII 227 GGCGCT [GA]GCGC^[CT]
HinfI 754 GAGTC G^A[ACGT]TC
MluI 653 ACGCGT A^CGCGT
NcoI 144 CCATGG C^CATGG
NlaIII 145 CATG CATG^
PflMI 191 CCAGTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 754 GAGTC GAGTC(4/5)
PstI 598 CTGCAG CTGCA^G
SapI 81 GCTCTTC GCTCTTC(1/4)
SmaI 161 CCCGGG CCC^GGG
Tsp509I 632 AATT ^AATT
Van91I 191 CCAGTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 59 CCAGCCCTATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 161 CCCGGG C^CCGGG
XmaI 161 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 654 CGCG CG^CG
664 CGCG
AcyI 412 GACGTC G[GA]^CG[CT]C
675 GACGCC
AfaI 489 GTAC GT^AC
570 GTAC
AvaI 161 CCCGGG C^[CT]CG[GA]G
526 CTCGGG
BfaI 379 CTAG C^TAG
485 CTAG
BsaHI 412 GACGTC G[GA]^CG[CT]C
675 GACGCC
BsmAI 202 GTCTC GTCTC(1/5)
756 GTCTC
BsoBI 161 CCCGGG C^[CT]CG[GA]G
526 CTCGGG
BstUI 654 CGCG CG^CG
664 CGCG
DpnII 264 GATC ^GATC
513 GATC
EarI 82 CTCTTC CTCTTC(1/4)
87 CTCTTC
Hin1I 412 GACGTC G[GA]^CG[CT]C
675 GACGCC
HphI 165 GGTGA GGTGA(8/7)
221 TCACC
Ksp632I 82 CTCTTC CTCTTC(1/4)
87 CTCTTC
MaeI 379 CTAG C^TAG
485 CTAG
MboI 264 GATC ^GATC
513 GATC
MvnI 654 CGCG CG^CG
664 CGCG
NdeII 264 GATC ^GATC
513 GATC
PpuMI 307 GGGACCT [GA]G^G[AT]CC[CT]
338 GGGACCC
RsaI 489 GTAC GT^AC
570 GTAC
Sau3AI 264 GATC ^GATC
513 GATC
SfaNI 253 GATGC GCATC(5/9)
323 GCATC
SfcI 384 CTACAG C^T[GA][CT]AG
598 CTGCAG
TaqI 262 TCGA T^CGA
658 TCGA
TthHB8I 262 TCGA T^CGA
658 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AluI AvaII BanII BbvI
BcnI BmyI BsaJI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DraII EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HpaII ItaI MaeII MaeIII
MboII MnlI MspI MvaI MwoI
NciI NlaIV Sau96I ScrFI StyI
TseI Tsp45I TspRI