Restriction map for
pFN21AE3939
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# NO CUT ENZYMES ( 138 enzymes) #
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AatII Acc65I AclI AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AvaI AviII
AvrII BalI BanI BbeI BbvCI
BcgI BciVI BclI BglI BglII
BlnI BlpI Bpu1102I BsaI BseRI
BsiEI BsiWI BsiYI BslI BsmAI
BsmBI BsoBI Bsp1407I BspDI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HhaI HinP1I HincII HindII
HpaI HphI KasI KpnI Ksp632I
KspI MluI MluNI MscI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PaeR7I
PflMI PinAI PmeI PpuMI PshAI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 1433 GTCTAC GT^[AC][GT]AC
AccII 21 CGCG CG^CG
AccIII 899 TCCGGA T^CCGGA
AciI 22 GCGG CCGC(-3/-1)
AcyI 919 GACGCC G[GA]^CG[CT]C
AflII 75 CTTAAG C^TTAAG
AflIII 26 ACACGT A^C[GA][CT]GT
AlwNI 707 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 187 ATTAAT AT^TAAT
AsnI 187 ATTAAT AT^TAAT
Asp700I 1501 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 30 GTGCTC G[AT]GC[AT]^C
AspI 1243 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 418 GGATCC G^GATCC
BanII 1022 GAGCCC G[GA]GC[CT]^C
BbrPI 27 CACGTG CAC^GTG
BcnI 813 CCGGG CC^[GC]GG
BfrI 75 CTTAAG C^TTAAG
BpmI 696 CTCCAG CTGGAG(16/14)
BsaAI 27 CACGTG [CT]AC^GT[GA]
BsaBI 709 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 919 GACGCC G[GA]^CG[CT]C
BsaWI 899 TCCGGA [AT]^CCGG[AT]
BseAI 899 TCCGGA T^CCGGA
BsgI 761 CTGCAC GTGCAG(16/14)
BsiHKAI 30 GTGCTC G[AT]GC[AT]^C
BsmI 517 GAATGC GAATGC(1/-1)
BspEI 899 TCCGGA T^CCGGA
BspHI 141 TCATGA T^CATGA
BssSI 1331 CTCGTG CACGAG(-5/-1)
BstUI 21 CGCG CG^CG
DsaI 1117 CCATGG C^C[GA][CT]GG
HgaI 919 GACGC GACGC(5/10)
Hin1I 919 GACGCC G[GA]^CG[CT]C
HindIII 579 AAGCTT A^AGCTT
MamI 709 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 615 CAATTG C^AATTG
MroI 899 TCCGGA T^CCGGA
MunI 615 CAATTG C^AATTG
MvnI 21 CGCG CG^CG
NciI 813 CCGGG CC^[GC]GG
NcoI 1117 CCATGG C^CATGG
PacI 184 TTAATTAA TTAAT^TAA
PflFI 1243 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1243 GACTC GAGTC(4/5)
PmaCI 27 CACGTG CAC^GTG
PmlI 27 CACGTG CAC^GTG
PshBI 187 ATTAAT AT^TAAT
RcaI 141 TCATGA T^CATGA
SspI 891 AATATT AAT^ATT
Tsp45I 821 GTGAC ^GT[GC]AC
Tth111I 1243 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1501 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AcsI 1135 GAATTT [GA]^AATT[CT]
1406 AAATTC
AfaI 283 GTAC GT^AC
1062 GTAC
ApoI 1135 GAATTT [GA]^AATT[CT]
1406 AAATTC
AvaII 1114 GGACC G^G[AT]CC
1412 GGTCC
BfaI 574 CTAG C^TAG
886 CTAG
BmyI 30 GTGCTC G[AGT]GC[ACT]^C
1022 GAGCCC
Bsp1286I 30 GTGCTC G[AGT]GC[ACT]^C
1022 GAGCCC
BsrDI 651 CATTGC GCAATG(2/0)
62 GCAATG
Cac8I 569 GCAAGC GC[ACGT]^[ACGT]GC
928 GCGTGC
EcoT14I 626 CCTTGG C^C[AT][AT]GG
1117 CCATGG
HapII 813 CCGG C^CGG
900 CCGG
HpaII 813 CCGG C^CGG
900 CCGG
MaeI 574 CTAG C^TAG
886 CTAG
MaeIII 744 GTTAC ^GT[ACGT]AC
821 GTGAC
MslI 213 CATTAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1126 CATATATATG
MspI 813 CCGG C^CGG
900 CCGG
MwoI 1150 GCAATTAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1336 GCCTCAAACGC
RsaI 283 GTAC GT^AC
1062 GTAC
StyI 626 CCTTGG C^C[AT][AT]GG
1117 CCATGG
TaqI 107 TCGA T^CGA
1075 TCGA
TthHB8I 107 TCGA T^CGA
1075 TCGA
XcmI 423 CCATTCTATGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
522 CCAGGATGACTTTGG
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AluI AlwI BbsI BbvI BpuAI
BsaJI BsmFI BsrI BssKI BstF5I
BstNI BstYI Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HinfI
ItaI MaeII MboI MboII MflI
MnlI MseI MspA1I MvaI NdeII
NlaIII NlaIV PvuII Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TfiI
Tru9I TseI Tsp509I TspRI XhoII