Restriction map for
pFN21AE1483
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# NO CUT ENZYMES ( 131 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI AlwI
AlwNI AocI Aor51HI ApaI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BbrPI BbsI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BpuAI BsaAI BsaBI
BsaWI BseAI BsgI BsiEI BsiWI
BsmFI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KpnI KspI MaeI
MamI MfeI MflI MluI MroI
MunI MvnI NaeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tth111I XbaI XhoI
XhoII
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcyI 519 GGCGCC G[GA]^CG[CT]C
Alw44I 384 GTGCAC G^TGCAC
ApaLI 384 GTGCAC G^TGCAC
Asp700I 431 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 519 GGCGCC GGCGC^C
BbvCI 1153 GCTGAGG CCTCAGC(-5/-2)
BglI 1040 GCCCCACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1131 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1131 GCTGAGC GC^T[ACGT]AGC
BsaHI 519 GGCGCC G[GA]^CG[CT]C
BssSI 349 CTCGTG CACGAG(-5/-1)
BstAPI 50 GCAATGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1131 GCTGAGC GC^T[ACGT]AGC
CfoI 520 GCGC GCG^C
EarI 256 CTCTTC CTCTTC(1/4)
FauI 788 CCCGC CCCGC(4/6)
HaeII 519 GGCGCC [GA]GCGC^[CT]
HgaI 660 GCGTC GACGC(5/10)
HhaI 520 GCGC GCG^C
Hin1I 519 GGCGCC G[GA]^CG[CT]C
HinP1I 520 GCGC G^CGC
HincII 748 GTTGAC GT[CT]^[GA]AC
HindII 748 GTTGAC GT[CT]^[GA]AC
KasI 519 GGCGCC G^GCGCC
Ksp632I 256 CTCTTC CTCTTC(1/4)
MseI 688 TTAA T^TAA
MslI 1080 CACTGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 536 CAGCTG C[AC]G^C[GT]G
NarI 519 GGCGCC GG^CGCC
NcoI 924 CCATGG C^CATGG
NdeI 812 CATATG CA^TATG
PflMI 1023 CCAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 631 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 536 CAGCTG CAG^CTG
SacI 64 GAGCTC GAGCT^C
SapI 255 GCTCTTC GCTCTTC(1/4)
SexAI 773 ACCTGGT A^CC[AT]GGT
SfoI 519 GGCGCC GGC^GCC
SmaI 188 CCCGGG CCC^GGG
SmlI 1282 CTTGAG C^T[CT][GA]AG
SphI 1127 GCATGC GCATG^C
StuI 1157 AGGCCT AGG^CCT
TaqI 650 TCGA T^CGA
Tru9I 688 TTAA T^TAA
TthHB8I 650 TCGA T^CGA
Van91I 1023 CCAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 188 CCCGGG C^CCGGG
XmaI 188 CCCGGG C^CCGGG
XmnI 431 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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AccI 202 GTAGAC GT^[AC][GT]AC
610 GTAGAC
AciI 249 CCGC CCGC(-3/-1)
789 CCGC
AcsI 362 GAATTC [GA]^AATT[CT]
760 GAATTC
ApoI 362 GAATTC [GA]^AATT[CT]
760 GAATTC
AvaI 188 CCCGGG C^[CT]CG[GA]G
311 CCCGAG
AvaII 357 GGACC G^G[AT]CC
1173 GGACC
BsaI 1097 GAGACC GGTCTC(1/5)
391 GGTCTC
BseRI 131 GAGGAG GAGGAG(10/8)
1297 GAGGAG
BsmBI 466 CGTCTC CGTCTC(1/5)
163 GAGACG
BsoBI 188 CCCGGG C^[CT]CG[GA]G
311 CCCGAG
DpnII 339 GATC ^GATC
870 GATC
DsaI 924 CCATGG C^C[GA][CT]GG
957 CCGTGG
EcoNI 1101 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1281 CCTTGAGAAGG
EcoRI 362 GAATTC G^AATTC
760 GAATTC
EcoT14I 924 CCATGG C^C[AT][AT]GG
1106 CCAAGG
HapII 189 CCGG C^CGG
517 CCGG
HinfI 139 GATTC G^A[ACGT]TC
1312 GATTC
HpaII 189 CCGG C^CGG
517 CCGG
MboI 339 GATC ^GATC
870 GATC
MspI 189 CCGG C^CGG
517 CCGG
NdeII 339 GATC ^GATC
870 GATC
NspI 180 GCATGT [GA]CATG^[CT]
1127 GCATGC
Sau3AI 339 GATC ^GATC
870 GATC
SfcI 539 CTGTAG C^T[GA][CT]AG
860 CTGTAG
StyI 924 CCATGG C^C[AT][AT]GG
1106 CCAAGG
TfiI 139 GATTC G^A[AT]TC
1312 GATTC
XcmI 414 CCACAGCTTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1019 CCATCCAGTTTGTGG
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# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AfaI AluI AspHI BalI BanI
BanII BbvI BcnI BmyI BpmI
BsaJI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstXI Cac8I Cfr13I DdeI
EaeI EcoRII Fnu4HI FokI HaeIII
HphI ItaI MaeII MaeIII MboII
MluNI MnlI MscI MvaI MwoI
NciI NlaIII NlaIV RsaI Sau96I
ScrFI SfaNI TseI Tsp45I Tsp509I
TspRI