Restriction map for
pFN21AE6433
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII AccIII AclI AflII Aor51HI
AscI AseI AsnI Asp700I AviII
BalI BamHI BbeI BbrPI BbvCI
BcgI BfrI BglI BglII BlpI
Bpu1102I BsaAI BsaBI BseAI BseRI
BsgI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BsrBI BsrGI BssHII
Bst1107I BstAPI BstEII BstPI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI Eco47III EcoO65I EcoRI EcoRV
FseI FspI HaeII HindIII HpaI
KasI MamI MfeI MluI MluNI
MroI MscI MslI MunI NarI
NcoI NdeI NheI NruI PacI
PaeR7I PflMI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Van91I
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 64 enzymes) #
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Acc65I 269 GGTACC G^GTACC
AcyI 127 GACGCC G[GA]^CG[CT]C
AgeI 272 ACCGGT A^CCGGT
AhdI 460 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 999 GTGCAC G^TGCAC
AocI 309 CCTGAGG CC^T[ACGT]AGG
ApaI 338 GGGCCC GGGCC^C
ApaLI 999 GTGCAC G^TGCAC
Asp718I 269 GGTACC G^GTACC
AspEI 460 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 433 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1108 CCTAGG C^CTAGG
BanII 338 GGGCCC G[GA]GC[CT]^C
BciVI 642 GTATCC GTATCC(6/5)
BclI 300 TGATCA T^GATCA
BlnI 1108 CCTAGG C^CTAGG
BsaHI 127 GACGCC G[GA]^CG[CT]C
BspLU11I 1214 ACATGT A^CATGT
BsrDI 80 GCAATG GCAATG(2/0)
BssSI 43 CTCGTG CACGAG(-5/-1)
BstBI 413 TTCGAA TT^CGAA
BstXI 1075 CCAAAATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1112 GGATCT [GA]^GATC[CT]
Bsu36I 309 CCTGAGG CC^T[ACGT]AGG
CfoI 139 GCGC GCG^C
DsaI 156 CCGCGG C^C[GA][CT]GG
EagI 153 CGGCCG C^GGCCG
Eam1105I 460 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 153 CGGCCG C^GGCCG
Eco52I 153 CGGCCG C^GGCCG
Eco81I 309 CCTGAGG CC^T[ACGT]AGG
EcoNI 771 CCTAGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1108 CCTAGG C^C[AT][AT]GG
EcoT22I 1138 ATGCAT ATGCA^T
FbaI 300 TGATCA T^GATCA
HhaI 139 GCGC GCG^C
Hin1I 127 GACGCC G[GA]^CG[CT]C
HinP1I 139 GCGC G^CGC
HincII 468 GTCGAC GT[CT]^[GA]AC
HindII 468 GTCGAC GT[CT]^[GA]AC
HphI 498 GGTGA GGTGA(8/7)
KpnI 269 GGTACC GGTAC^C
KspI 156 CCGCGG CCGC^GG
MflI 1112 GGATCT [GA]^GATC[CT]
MseI 1195 TTAA T^TAA
NaeI 148 GCCGGC GCC^GGC
NgoMIV 148 GCCGGC G^CCGGC
NotI 152 GCGGCCGC GC^GGCCGC
NsiI 1138 ATGCAT ATGCA^T
NspI 1214 ACATGT [GA]CATG^[CT]
NspV 413 TTCGAA TT^CGAA
PflFI 433 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 272 ACCGGT A^CCGGT
PpuMI 874 GGGACCT [GA]G^G[AT]CC[CT]
SacII 156 CCGCGG CCGC^GG
SalI 468 GTCGAC G^TCGAC
SfuI 413 TTCGAA TT^CGAA
SmaI 344 CCCGGG CCC^GGG
StyI 1108 CCTAGG C^C[AT][AT]GG
Tru9I 1195 TTAA T^TAA
Tth111I 433 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 1112 GGATCT [GA]^GATC[CT]
XmaCI 344 CCCGGG C^CCGGG
XmaI 344 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 468 GTCGAC GT^[AC][GT]AC
1157 GTAGAC
AflIII 206 ACGTGT A^C[GA][CT]GT
1214 ACATGT
AlwI 780 GGATC GGATC(4/5)
1112 GGATC
AlwNI 304 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
985 CAGAAGCTG
AspHI 946 GAGCAC G[AT]GC[AT]^C
999 GTGCAC
AvaI 244 CCCGAG C^[CT]CG[GA]G
344 CCCGGG
BpmI 72 CTGGAG CTGGAG(16/14)
1124 CTGGAG
BsaI 593 GAGACC GGTCTC(1/5)
198 GGTCTC
BsaWI 272 ACCGGT [AT]^CCGG[AT]
510 TCCGGT
BsiEI 153 CGGCCG CG[GA][CT]^CG
470 CGACCG
BsiHKAI 946 GAGCAC G[AT]GC[AT]^C
999 GTGCAC
BsoBI 244 CCCGAG C^[CT]CG[GA]G
344 CCCGGG
BspMI 596 ACCTGC ACCTGC(4/8)
519 GCAGGT
BstF5I 189 CATCC GGATG(2/0)
1163 CATCC
EarI 1046 CTCTTC CTCTTC(1/4)
775 GAAGAG
FauI 136 CCCGC CCCGC(4/6)
141 GCGGG
FokI 189 CATCC GGATG(9/13)
1163 CATCC
Ksp632I 1046 CTCTTC CTCTTC(1/4)
775 GAAGAG
MaeII 206 ACGT A^CGT
437 ACGT
MaeIII 572 GTGAC ^GT[ACGT]AC
1188 GTCAC
PstI 517 CTGCAG CTGCA^G
1336 CTGCAG
PvuII 289 CAGCTG CAG^CTG
484 CAGCTG
Tsp45I 572 GTGAC ^GT[GC]AC
1188 GTCAC
TspRI 697 CACTG CA[GC]TG[ACGT][ACGT]^
1239 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI AvaII BanI BbsI BbvI
BcnI BfaI BmyI BpuAI BsaJI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BsrFI BsrI BssKI BstNI
BstUI Cac8I Cfr10I Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
Fnu4HI HaeIII HapII HgaI HinfI
HpaII ItaI MaeI MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TseI Tsp509I
TthHB8I