Restriction map for
pFN21AE1441
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbrPI BbsI BbvCI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaWI BseAI BsiWI BspDI BspEI
BspHI BspLU11I BspMI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I CelII ClaI CpoI
DraI DrdI Eam1105I Eco47III EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI MamI MfeI MluI
MroI MunI NdeI NheI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmlI SnaBI SpeI Sse8387I SspI
SwaI Tsp509I Tth111I Van91I XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 373 GACGTC GACGT^C
AlwI 504 GATCC GGATC(4/5)
AocI 636 CCTGAGG CC^T[ACGT]AGG
ApaI 1183 GGGCCC GGGCC^C
AvaI 601 CCCGGG C^[CT]CG[GA]G
BcgI 1188 CGACGGGCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1185 GCCCGACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI 670 CTGCAC GTGCAG(16/14)
BsmBI 371 GAGACG CGTCTC(1/5)
BsmI 748 GCATTC GAATGC(1/-1)
BsoBI 601 CCCGGG C^[CT]CG[GA]G
BsrDI 279 GCAATG GCAATG(2/0)
BsrFI 1051 GCCGGC [GA]^CCGG[CT]
BstF5I 477 CATCC GGATG(2/0)
BstYI 503 AGATCC [GA]^GATC[CT]
Bsu36I 636 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1051 GCCGGC [GA]^CCGG[CT]
DpnII 504 GATC ^GATC
DraII 716 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 868 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 660 CGGCCG C^GGCCG
EarI 103 CTCTTC CTCTTC(1/4)
EclXI 660 CGGCCG C^GGCCG
Eco52I 660 CGGCCG C^GGCCG
Eco81I 636 CCTGAGG CC^T[ACGT]AGG
EcoO109I 716 AGGCCCC [GA]G^G[ACGT]CC[CT]
FokI 477 CATCC GGATG(9/13)
HphI 681 TCACC GGTGA(8/7)
Ksp632I 103 CTCTTC CTCTTC(1/4)
KspI 521 CCGCGG CCGC^GG
MboI 504 GATC ^GATC
MflI 503 AGATCC [GA]^GATC[CT]
NaeI 1051 GCCGGC GCC^GGC
NcoI 274 CCATGG C^CATGG
NdeII 504 GATC ^GATC
NgoMIV 1051 GCCGGC G^CCGGC
NotI 659 GCGGCCGC GC^GGCCGC
NspI 1194 GCATGC [GA]CATG^[CT]
PshAI 428 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 521 CCGCGG CCGC^GG
Sau3AI 504 GATC ^GATC
SfaNI 536 GCATC GCATC(5/9)
SmaI 601 CCCGGG CCC^GGG
SphI 1194 GCATGC GCATG^C
StuI 952 AGGCCT AGG^CCT
XbaI 417 TCTAGA T^CTAGA
XhoII 503 AGATCC [GA]^GATC[CT]
XmaCI 601 CCCGGG C^CCGGG
XmaI 601 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 376 GTCTAC GT^[AC][GT]AC
802 GTCTAC
BalI 68 TGGCCA TGG^CCA
686 TGGCCA
BanII 6 GGGCTC G[GA]GC[CT]^C
1183 GGGCCC
BbeI 525 GGCGCC GGCGC^C
1163 GGCGCC
BfaI 418 CTAG C^TAG
1147 CTAG
BsaI 895 GAGACC GGTCTC(1/5)
1209 GAGACC
BsiEI 489 CGACCG CG[GA][CT]^CG
660 CGGCCG
Bsp1407I 313 TGTACA T^GTACA
842 TGTACA
BsrBI 180 GAGCGG CCGCTC(-3/-3)
657 GAGCGG
BsrGI 313 TGTACA T^GTACA
842 TGTACA
EcoT14I 167 CCTTGG C^C[AT][AT]GG
274 CCATGG
KasI 525 GGCGCC G^GCGCC
1163 GGCGCC
MaeI 418 CTAG C^TAG
1147 CTAG
MluNI 68 TGGCCA TGG^CCA
686 TGGCCA
MscI 68 TGGCCA TGG^CCA
686 TGGCCA
MseI 232 TTAA T^TAA
997 TTAA
NarI 525 GGCGCC GG^CGCC
1163 GGCGCC
SfoI 525 GGCGCC GGC^GCC
1163 GGCGCC
SspBI 313 TGTACA T^GTACA
842 TGTACA
StyI 167 CCTTGG C^C[AT][AT]GG
274 CCATGG
Tru9I 232 TTAA T^TAA
997 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AspHI AvaII BanI BbvI
BcnI BmyI BpmI BsaHI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DsaI EaeI EcoRII FauI Fnu4HI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII ItaI
MaeII MaeIII MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIII NlaIV PleI PstI
PvuII RsaI Sau96I ScrFI SfcI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I