Restriction map for
pFN21AE3664
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AlwI AocI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII BamHI BbeI BbvCI BcgI
BciVI BclI BfrI BglII BsaBI
BsaWI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI Bst1107I BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco81I EcoNI EcoO65I EcoRI EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KpnI MamI
MfeI MflI MluI MroI MseI
MslI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tth111I XbaI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AcyI 1121 GACGCC G[GA]^CG[CT]C
AfaI 1919 GTAC GT^AC
Alw44I 1696 GTGCAC G^TGCAC
ApaLI 1696 GTGCAC G^TGCAC
BanI 1570 GGCACC G^G[CT][GA]CC
BbrPI 607 CACGTG CAC^GTG
BbsI 1413 GTCTTC GAAGAC(2/6)
BglI 1640 GCCACCTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 696 GCTGAGC GC^T[ACGT]AGC
BpmI 1600 CTGGAG CTGGAG(16/14)
Bpu1102I 696 GCTGAGC GC^T[ACGT]AGC
BpuAI 1413 GTCTTC GAAGAC(2/6)
BsaAI 607 CACGTG [CT]AC^GT[GA]
BsaHI 1121 GACGCC G[GA]^CG[CT]C
BsiEI 535 CGGCCG CG[GA][CT]^CG
BsrFI 368 GCCGGC [GA]^CCGG[CT]
BssHII 1675 GCGCGC G^CGCGC
BssSI 1184 CTCGTG CACGAG(-5/-1)
BstAPI 213 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 696 GCTGAGC GC^T[ACGT]AGC
Cfr10I 368 GCCGGC [GA]^CCGG[CT]
EagI 535 CGGCCG C^GGCCG
EclXI 535 CGGCCG C^GGCCG
Eco52I 535 CGGCCG C^GGCCG
EcoRV 1030 GATATC GAT^ATC
Hin1I 1121 GACGCC G[GA]^CG[CT]C
HinfI 328 GACTC G^A[ACGT]TC
MaeII 608 ACGT A^CGT
NaeI 368 GCCGGC GCC^GGC
NgoMIV 368 GCCGGC G^CCGGC
NspI 1671 GCATGC [GA]CATG^[CT]
PleI 328 GACTC GAGTC(4/5)
PmaCI 607 CACGTG CAC^GTG
PmlI 607 CACGTG CAC^GTG
PvuII 1795 CAGCTG CAG^CTG
RsaI 1919 GTAC GT^AC
SexAI 1655 ACCTGGT A^CC[AT]GGT
SfcI 1891 CTACAG C^T[GA][CT]AG
SphI 1671 GCATGC GCATG^C
Tsp509I 1476 AATT ^AATT
XcmI 153 CCATGGCGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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Aor51HI 452 AGCGCT AGC^GCT
1043 AGCGCT
AvrII 79 CCTAGG C^CTAGG
1644 CCTAGG
BlnI 79 CCTAGG C^CTAGG
1644 CCTAGG
BsaI 288 GGTCTC GGTCTC(1/5)
729 GGTCTC
BseRI 148 CTCCTC GAGGAG(10/8)
22 GAGGAG
BsmBI 1455 CGTCTC CGTCTC(1/5)
737 GAGACG
BsrBI 145 CCGCTC CCGCTC(-3/-3)
1834 CCGCTC
EarI 1271 CTCTTC CTCTTC(1/4)
430 GAAGAG
Eco47III 452 AGCGCT AGC^GCT
1043 AGCGCT
Ksp632I 1271 CTCTTC CTCTTC(1/4)
430 GAAGAG
KspI 1116 CCGCGG CCGC^GG
1808 CCGCGG
PflMI 939 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1483 CCATCCTCTGG
SacII 1116 CCGCGG CCGC^GG
1808 CCGCGG
TaqI 893 TCGA T^CGA
1682 TCGA
TthHB8I 893 TCGA T^CGA
1682 TCGA
Van91I 939 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1483 CCATCCTCTGG
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# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AluI AlwNI ApaI
AspHI AvaI AvaII BalI BanII
BbvI BcnI BfaI BmyI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HpaII
HphI ItaI MaeI MaeIII MboI
MboII MluNI MnlI MscI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI SmaI StyI
TseI Tsp45I TspRI XmaCI XmaI