Restriction map for
pFN21AE1112
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# NO CUT ENZYMES ( 180 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI AlwI AlwNI AocI
Aor51HI ApaI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AvaI
AviII AvrII BalI BamHI BanI
BanII BbeI BbsI BbvCI BbvI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BsrI BssSI Bst1107I BstAPI
BstBI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DpnII DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HaeII Hin1I HinfI
HpaI KasI KpnI Ksp632I KspI
MaeI MamI MboI MboII MfeI
MflI MluI MluNI MroI MscI
MslI MspA1I MunI NaeI NarI
NcoI NdeI NdeII NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PleI PmeI
PpuMI PshBI Psp1406I PstI PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfaNI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TfiI
TseI Tsp509I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Alw44I 364 GTGCAC G^TGCAC
ApaLI 364 GTGCAC G^TGCAC
AspI 283 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 282 GGACC G^G[AT]CC
BbrPI 217 CACGTG CAC^GTG
BcnI 285 CCCGG CC^[GC]GG
BpmI 88 CTGGAG CTGGAG(16/14)
BsaAI 217 CACGTG [CT]AC^GT[GA]
BsgI 259 CTGCAC GTGCAG(16/14)
BsmAI 9 GTCTC GTCTC(1/5)
BspMI 257 ACCTGC ACCTGC(4/8)
BssHII 56 GCGCGC G^CGCGC
BstEII 321 GGTGACC G^GT[ACGT]ACC
BstF5I 95 GGATG GGATG(2/0)
BstPI 321 GGTGACC G^GT[ACGT]ACC
BstXI 203 CCAACGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 83 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 83 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 321 GGTGACC G^GT[ACGT]ACC
FokI 95 GGATG GGATG(9/13)
HapII 286 CCGG C^CGG
HgaI 193 GACGC GACGC(5/10)
HincII 289 GTCAAC GT[CT]^[GA]AC
HindII 289 GTCAAC GT[CT]^[GA]AC
HindIII 271 AAGCTT A^AGCTT
HpaII 286 CCGG C^CGG
MaeIII 322 GTGAC ^GT[ACGT]AC
MseI 167 TTAA T^TAA
MspI 286 CCGG C^CGG
NciI 285 CCCGG CC^[GC]GG
NlaIII 228 CATG CATG^
NlaIV 282 GGACCC GG[ACGT]^[ACGT]CC
NspI 227 ACATGC [GA]CATG^[CT]
PflFI 283 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 217 CACGTG CAC^GTG
PmlI 217 CACGTG CAC^GTG
PshAI 24 GACCAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
TaqI 140 TCGA T^CGA
Tru9I 167 TTAA T^TAA
Tsp45I 322 GTGAC ^GT[GC]AC
TspRI 310 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 283 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 140 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AluI 272 AGCT AG^CT
299 AGCT
AspHI 364 GTGCAC G[AT]GC[AT]^C
399 GAGCAC
BcgI 69 CGAGTATGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
267 GCACAAGCTTCG
BmyI 364 GTGCAC G[AGT]GC[ACT]^C
399 GAGCAC
BsiHKAI 364 GTGCAC G[AT]GC[AT]^C
399 GAGCAC
BsiYI 86 CCCTGGAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
162 CCAGGTTAAGG
BslI 86 CCCTGGAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
162 CCAGGTTAAGG
Bsp1286I 364 GTGCAC G[AGT]GC[ACT]^C
399 GAGCAC
EaeI 188 TGGCCG [CT]^GGCC[GA]
329 TGGCCG
FauI 132 CCCGC CCCGC(4/6)
343 CCCGC
Fnu4HI 37 GCCGC GC^[ACGT]GC
331 GCCGC
HphI 321 GGTGA GGTGA(8/7)
353 TCACC
ItaI 37 GCCGC GC^[ACGT]GC
331 GCCGC
MaeII 29 ACGT A^CGT
218 ACGT
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AccII AciI BsaJI BssKI BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DsaI EcoRII HaeIII HhaI HinP1I
MnlI MvaI MvnI MwoI Sau96I
ScrFI