Restriction map for
pFN21AE1393
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# NO CUT ENZYMES ( 206 enzymes) #
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Acc65I AccII AccIII AciI AclI
AflII AflIII AgeI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspHI AspI AvaI AvaII AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI BmyI Bpu1102I
BpuAI BsaAI BsaBI BsaJI BsaWI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I ClaI CpoI
DpnII DraI DraII DraIII DrdI
DsaI EaeI EagI EarI EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRI EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HaeIII HapII
HgaI HhaI HinP1I HindIII HinfI
HpaII KasI KpnI Ksp632I KspI
MaeIII MamI MboI MfeI MflI
MluI MluNI MroI MscI MspA1I
MspI MunI MvnI MwoI NaeI
NarI NciI NcoI NdeI NdeII
NgoMIV NheI NlaIV NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
Sau3AI Sau96I SbfI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI Tsp45I Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AcsI 72 GAATTT [GA]^AATT[CT]
AfaI 54 GTAC GT^AC
AhdI 117 GACCGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI 11 AGCT AG^CT
ApoI 72 GAATTT [GA]^AATT[CT]
AspEI 117 GACCGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvI 241 GCAGC GCAGC(8/12)
BfaI 112 CTAG C^TAG
BpmI 13 CTGGAG CTGGAG(16/14)
BsaI 115 GAGACC GGTCTC(1/5)
BsmAI 115 GAGAC GTCTC(1/5)
BssKI 204 CCAGG ^CC[ACGT]GG
BstF5I 93 GGATG GGATG(2/0)
BstNI 204 CCAGG CC^[AT]GG
DdeI 128 CTCAG C^T[ACGT]AG
Eam1105I 117 GACCGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 60 CCTGATAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 204 CCAGG ^CC[AT]GG
EcoRV 175 GATATC GAT^ATC
Fnu4HI 241 GCAGC GC^[ACGT]GC
FokI 93 GGATG GGATG(9/13)
HpaI 196 GTTAAC GTT^AAC
HphI 222 GGTGA GGTGA(8/7)
ItaI 241 GCAGC GC^[ACGT]GC
MaeI 112 CTAG C^TAG
MseI 197 TTAA T^TAA
MslI 216 CATTCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 204 CCAGG CC^[AT]GG
RsaI 54 GTAC GT^AC
ScaI 53 AGTACT AGT^ACT
ScrFI 204 CCAGG CC^[ACGT]GG
SfcI 87 CTACAG C^T[GA][CT]AG
TaqI 38 TCGA T^CGA
Tru9I 197 TTAA T^TAA
TseI 241 GCAGC G^C[AT]GC
TspRI 183 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 38 TCGA T^CGA
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# TWO CUT ENZYMES ( 13 enzymes) #
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AatII 40 GACGTC GACGT^C
154 GACGTC
AccI 43 GTCTAC GT^[AC][GT]AC
85 GTCTAC
AcyI 40 GACGTC G[GA]^CG[CT]C
154 GACGTC
BsaHI 40 GACGTC G[GA]^CG[CT]C
154 GACGTC
BsgI 149 GTGCAG GTGCAG(16/14)
191 GTGCAG
Hin1I 40 GACGTC G[GA]^CG[CT]C
154 GACGTC
HincII 3 GTTGAC GT[CT]^[GA]AC
196 GTTAAC
HindII 3 GTTGAC GT[CT]^[GA]AC
196 GTTAAC
MaeII 41 ACGT A^CGT
155 ACGT
MboII 102 GAAGA GAAGA(8/7)
262 GAAGA
MnlI 127 CCTC CCTC(7/6)
213 CCTC
NlaIII 79 CATG CATG^
259 CATG
Tsp509I 73 AATT ^AATT
107 AATT
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# 3 OR MORE CUT ENZYMES ( 2 enzymes) #
#########################################
BsiYI BslI