Restriction map for
pFN21AE6241
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AvaII AviII AvrII BalI
BbeI BbrPI BbsI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrFI BsrGI BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CelII Cfr10I Cfr13I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EarI EclXI Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HincII HindII HindIII HpaI
HphI KasI KpnI Ksp632I MaeI
MaeIII MamI MfeI MluI MluNI
MroI MscI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SalI
SapI Sau96I SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspBI
StuI StyI SwaI Tsp45I Tth111I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AfaI 340 GTAC GT^AC
Aor51HI 170 AGCGCT AGC^GCT
Asp700I 231 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 225 GAGCTC G[AT]GC[AT]^C
BamHI 56 GGATCC G^GATCC
BbvCI 502 CCTCAGC CCTCAGC(-5/-2)
BcnI 188 CCCGG CC^[GC]GG
BpmI 484 CTCCAG CTGGAG(16/14)
BsaAI 292 TACGTG [CT]AC^GT[GA]
BsaI 68 GGTCTC GGTCTC(1/5)
BsgI 539 CTGCAC GTGCAG(16/14)
BsiHKAI 225 GAGCTC G[AT]GC[AT]^C
BspMI 394 GCAGGT ACCTGC(4/8)
BsrBI 259 GAGCGG CCGCTC(-3/-3)
BsrDI 200 CATTGC GCAATG(2/0)
BssHII 245 GCGCGC G^CGCGC
BstXI 363 CCAGACTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EaeI 296 TGGCCG [CT]^GGCC[GA]
Eco47III 170 AGCGCT AGC^GCT
HapII 189 CCGG C^CGG
HpaII 189 CCGG C^CGG
KspI 211 CCGCGG CCGC^GG
MaeII 293 ACGT A^CGT
MslI 288 CACTTACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 189 CCGG C^CGG
NciI 188 CCCGG CC^[GC]GG
PflMI 580 CCAGTAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 340 GTAC GT^AC
SacI 225 GAGCTC GAGCT^C
SacII 211 CCGCGG CCGC^GG
SfaNI 192 GCATC GCATC(5/9)
SspI 604 AATATT AAT^ATT
Van91I 580 CCAGTAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 231 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AcsI 590 GAATTT [GA]^AATT[CT]
724 GAATTT
AcyI 14 GGCGTC G[GA]^CG[CT]C
324 GGCGTC
ApoI 590 GAATTT [GA]^AATT[CT]
724 GAATTT
BglI 207 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
210 GCCGCGGCGGC
BsaHI 14 GGCGTC G[GA]^CG[CT]C
324 GGCGTC
BseRI 253 GAGGAG GAGGAG(10/8)
256 GAGGAG
BsmFI 124 GGGAC GGGAC(10/14)
160 GGGAC
BstAPI 394 GCAGGTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
801 GCACAAGTTGC
BstF5I 193 CATCC GGATG(2/0)
22 GGATG
BstYI 56 GGATCC [GA]^GATC[CT]
735 AGATCC
DsaI 211 CCGCGG C^C[GA][CT]GG
308 CCGTGG
FokI 193 CATCC GGATG(9/13)
22 GGATG
HaeII 50 GGCGCT [GA]GCGC^[CT]
170 AGCGCT
HgaI 15 GCGTC GACGC(5/10)
325 GCGTC
Hin1I 14 GGCGTC G[GA]^CG[CT]C
324 GGCGTC
HinfI 443 GAATC G^A[ACGT]TC
564 GAATC
MboII 661 GAAGA GAAGA(8/7)
733 GAAGA
MflI 56 GGATCC [GA]^GATC[CT]
735 AGATCC
MspA1I 211 CCGCGG C[AC]G^C[GT]G
299 CCGCTG
NspI 314 GCATGC [GA]CATG^[CT]
474 GCATGC
PstI 83 CTGCAG CTGCA^G
174 CTGCAG
SfcI 83 CTGCAG C^T[GA][CT]AG
174 CTGCAG
SphI 314 GCATGC GCATG^C
474 GCATGC
TfiI 443 GAATC G^A[AT]TC
564 GAATC
XhoII 56 GGATCC [GA]^GATC[CT]
735 AGATCC
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# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
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AccII AciI AluI AlwI BanI
BanII BbvI BmyI BsaJI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstNI BstUI Cac8I CfoI DdeI
DpnII EcoRII Fnu4HI HaeIII HhaI
HinP1I ItaI MboI MnlI MseI
MvaI MvnI MwoI NdeII NlaIII
NlaIV Sau3AI ScrFI TaqI Tru9I
TseI Tsp509I TspRI TthHB8I