Restriction map for
pFN21AE2612
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AhdI Alw44I AlwNI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AviII BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BglII BsaAI BsaBI
BsaHI BsaI BsiEI BsiWI BsmAI
BsmBI Bsp1407I BspDI BspHI BsrGI
Bst1107I BstAPI BstBI BstYI BstZ17I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco52I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI Hin1I HindIII HpaI KasI
KpnI KspI MamI MflI MluI
NaeI NarI NdeI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflMI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SbfI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Van91I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccII 118 CGCG CG^CG
AccIII 739 TCCGGA T^CCGGA
AcsI 715 AAATTT [GA]^AATT[CT]
AgeI 707 ACCGGT A^CCGGT
AlwI 867 GGATC GGATC(4/5)
AocI 67 CCTGAGG CC^T[ACGT]AGG
Aor51HI 400 AGCGCT AGC^GCT
ApoI 715 AAATTT [GA]^AATT[CT]
AspHI 946 GAGCTC G[AT]GC[AT]^C
AspI 144 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 502 CCTAGG C^CTAGG
BanI 1021 GGCACC G^G[CT][GA]CC
BlnI 502 CCTAGG C^CTAGG
BlpI 1065 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1065 GCTCAGC GC^T[ACGT]AGC
BseAI 739 TCCGGA T^CCGGA
BsgI 97 GTGCAG GTGCAG(16/14)
BsiHKAI 946 GAGCTC G[AT]GC[AT]^C
BsmI 1014 GAATGC GAATGC(1/-1)
BspEI 739 TCCGGA T^CCGGA
BsrBI 547 GAGCGG CCGCTC(-3/-3)
BsrDI 929 CATTGC GCAATG(2/0)
BssHII 117 GCGCGC G^CGCGC
BstEII 45 GGTGACC G^GT[ACGT]ACC
BstPI 45 GGTGACC G^GT[ACGT]ACC
BstUI 118 CGCG CG^CG
Bsu36I 67 CCTGAGG CC^T[ACGT]AGG
CelII 1065 GCTCAGC GC^T[ACGT]AGC
DpnII 868 GATC ^GATC
DraII 764 AGGCCCT [GA]G^G[ACGT]CC[CT]
Eco47III 400 AGCGCT AGC^GCT
Eco81I 67 CCTGAGG CC^T[ACGT]AGG
EcoO109I 764 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 45 GGTGACC G^GT[ACGT]ACC
HincII 1074 GTTGAC GT[CT]^[GA]AC
HindII 1074 GTTGAC GT[CT]^[GA]AC
MaeII 1049 ACGT A^CGT
MboI 868 GATC ^GATC
MroI 739 TCCGGA T^CCGGA
MseI 248 TTAA T^TAA
MspA1I 238 CAGCTG C[AC]G^C[GT]G
MvnI 118 CGCG CG^CG
NcoI 914 CCATGG C^CATGG
NdeII 868 GATC ^GATC
NheI 787 GCTAGC G^CTAGC
PflFI 144 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 707 ACCGGT A^CCGGT
PshAI 48 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 238 CAGCTG CAG^CTG
SacI 946 GAGCTC GAGCT^C
SapI 948 GCTCTTC GCTCTTC(1/4)
Sau3AI 868 GATC ^GATC
ScaI 749 AGTACT AGT^ACT
TfiI 1135 GATTC G^A[AT]TC
Tru9I 248 TTAA T^TAA
Tth111I 144 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AflIII 74 ACATGT A^C[GA][CT]GT
659 ACATGT
AvaI 58 CCCGAG C^[CT]CG[GA]G
476 CTCGGG
BalI 335 TGGCCA TGG^CCA
620 TGGCCA
BanII 313 GAGCCC G[GA]GC[CT]^C
946 GAGCTC
BbsI 678 GAAGAC GAAGAC(2/6)
1043 GTCTTC
BcnI 177 CCGGG CC^[GC]GG
537 CCGGG
BmyI 313 GAGCCC G[AGT]GC[ACT]^C
946 GAGCTC
BpuAI 678 GAAGAC GAAGAC(2/6)
1043 GTCTTC
BseRI 735 CTCCTC GAGGAG(10/8)
61 GAGGAG
BsmFI 253 GGGAC GGGAC(10/14)
55 GTCCC
BsoBI 58 CCCGAG C^[CT]CG[GA]G
476 CTCGGG
Bsp1286I 313 GAGCCC G[AGT]GC[ACT]^C
946 GAGCTC
BspLU11I 74 ACATGT A^CATGT
659 ACATGT
BspMI 99 GCAGGT ACCTGC(4/8)
891 GCAGGT
BsrFI 707 ACCGGT [GA]^CCGG[CT]
980 GCCGGT
BssSI 7 CTCGTG CACGAG(-5/-1)
94 CTCGTG
BstXI 302 CCATCGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1071 CCAGTTGACTGG
Cfr10I 707 ACCGGT [GA]^CCGG[CT]
980 GCCGGT
DsaI 155 CCACGG C^C[GA][CT]GG
914 CCATGG
EaeI 335 TGGCCA [CT]^GGCC[GA]
620 TGGCCA
EarI 949 CTCTTC CTCTTC(1/4)
1147 GAAGAG
EcoNI 93 CCTCGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
756 CCTGAAAGAGG
FauI 37 GCGGG CCCGC(4/6)
243 GCGGG
HaeII 400 AGCGCT [GA]GCGC^[CT]
581 AGCGCC
Ksp632I 949 CTCTTC CTCTTC(1/4)
1147 GAAGAG
MfeI 519 CAATTG C^AATTG
920 CAATTG
MluNI 335 TGGCCA TGG^CCA
620 TGGCCA
MscI 335 TGGCCA TGG^CCA
620 TGGCCA
MunI 519 CAATTG C^AATTG
920 CAATTG
NciI 177 CCGGG CC^[GC]GG
537 CCGGG
SmlI 227 CTCAAG C^T[CT][GA]AG
886 CTCAAG
StuI 380 AGGCCT AGG^CCT
432 AGGCCT
XcmI 155 CCACGGGCAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
905 CCAATGAAGCCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BfaI BpmI BsaJI BsaWI BsiYI
BslI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeIII MboII MnlI
MslI MspI MvaI MwoI NlaIII
NlaIV NspI PleI RsaI Sau96I
ScrFI SfaNI StyI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I