Restriction map for
pFN21AE6705
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BfrI BglI BlnI BsaBI
BsaHI BseAI BsiEI BsiWI BspDI
BspEI BspLU11I BssHII Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CfoI
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FauI FseI FspI HaeII HhaI
Hin1I HinP1I HindIII HpaI KasI
KpnI KspI MamI MluI MroI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspI StuI SwaI XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccII 1489 CGCG CG^CG
AflIII 65 ACGTGT A^C[GA][CT]GT
ApaI 1621 GGGCCC GGGCC^C
BalI 760 TGGCCA TGG^CCA
BanI 2064 GGCACC G^G[CT][GA]CC
BanII 1621 GGGCCC G[GA]GC[CT]^C
BciVI 1956 GGATAC GTATCC(6/5)
BcnI 1829 CCCGG CC^[GC]GG
BglII 1762 AGATCT A^GATCT
BlpI 531 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 531 GCTAAGC GC^T[ACGT]AGC
BsaWI 1634 TCCGGT [AT]^CCGG[AT]
BsmBI 1479 GAGACG CGTCTC(1/5)
BsmI 1928 GCATTC GAATGC(1/-1)
BsrBI 1604 GAGCGG CCGCTC(-3/-3)
BsrDI 813 CATTGC GCAATG(2/0)
BsrFI 80 GCCGGC [GA]^CCGG[CT]
BssSI 1332 CACGAG CACGAG(-5/-1)
BstUI 1489 CGCG CG^CG
CelII 531 GCTAAGC GC^T[ACGT]AGC
Cfr10I 80 GCCGGC [GA]^CCGG[CT]
DsaI 757 CCATGG C^C[GA][CT]GG
EaeI 760 TGGCCA [CT]^GGCC[GA]
HgaI 1481 GACGC GACGC(5/10)
HincII 403 GTTGAC GT[CT]^[GA]AC
HindII 403 GTTGAC GT[CT]^[GA]AC
MfeI 347 CAATTG C^AATTG
MluNI 760 TGGCCA TGG^CCA
MscI 760 TGGCCA TGG^CCA
MspA1I 1019 CAGCTG C[AC]G^C[GT]G
MunI 347 CAATTG C^AATTG
MvnI 1489 CGCG CG^CG
NaeI 80 GCCGGC GCC^GGC
NciI 1829 CCCGG CC^[GC]GG
NcoI 757 CCATGG C^CATGG
NgoMIV 80 GCCGGC G^CCGGC
PflMI 286 CCAGCTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 1019 CAGCTG CAG^CTG
SbfI 2250 CCTGCAGG CCTGCA^GG
ScaI 1908 AGTACT AGT^ACT
SmlI 2081 CTCAAG C^T[CT][GA]AG
Sse8387I 2250 CCTGCAGG CCTGCA^GG
Van91I 286 CCAGCTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 1943 GAATTT [GA]^AATT[CT]
2087 AAATTC
AlwNI 1834 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
2292 CAGTGCCTG
ApoI 1943 GAATTT [GA]^AATT[CT]
2087 AAATTC
AspI 1661 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1842 GACCCAGTC
AvaI 1238 CTCGGG C^[CT]CG[GA]G
2032 CCCGAG
BclI 497 TGATCA T^GATCA
1062 TGATCA
BfaI 1357 CTAG C^TAG
1933 CTAG
BsaAI 355 TACGTG [CT]AC^GT[GA]
1114 TACGTG
BsaI 780 GAGACC GGTCTC(1/5)
1990 GAGACC
BseRI 1433 GAGGAG GAGGAG(10/8)
1615 GAGGAG
BsgI 653 CTGCAC GTGCAG(16/14)
517 GTGCAG
BsoBI 1238 CTCGGG C^[CT]CG[GA]G
2032 CCCGAG
Bsp1407I 159 TGTACA T^GTACA
1521 TGTACA
BsrGI 159 TGTACA T^GTACA
1521 TGTACA
BstYI 1359 AGATCC [GA]^GATC[CT]
1762 AGATCT
EarI 1147 CTCTTC CTCTTC(1/4)
777 GAAGAG
EcoNI 886 CCTACCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2055 CCTTCTGCAGG
FbaI 497 TGATCA T^GATCA
1062 TGATCA
HphI 309 GGTGA GGTGA(8/7)
1987 GGTGA
Ksp632I 1147 CTCTTC CTCTTC(1/4)
777 GAAGAG
MaeI 1357 CTAG C^TAG
1933 CTAG
MflI 1359 AGATCC [GA]^GATC[CT]
1762 AGATCT
NspI 409 ACATGC [GA]CATG^[CT]
2027 ACATGC
PflFI 1661 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1842 GACCCAGTC
SspBI 159 TGTACA T^GTACA
1521 TGTACA
Tth111I 1661 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1842 GACCCAGTC
XhoII 1359 AGATCC [GA]^GATC[CT]
1762 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BbsI BbvI BmyI BpmI
BpuAI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BspMI
BsrI BssKI BstAPI BstF5I BstNI
BstXI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
ItaI MaeII MaeIII MboI MboII
MnlI MseI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
PpuMI PstI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I