##################################
# NO CUT ENZYMES (  203 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BcnI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaJI       BseAI       BseRI       BsgI        BsiEI       
BsiWI       BsmBI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspLU11I    BspMI       BsrBI       
BsrFI       BsrGI       BsrI        BssHII      BssKI       
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstF5I      BstNI       BstPI       BstUI       BstYI       
BstZ17I     Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRII      EcoRV       EcoT14I     EcoT22I     FauI        
FokI        FseI        FspI        HaeII       HaeIII      
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HinfI       HpaI        KasI        
KpnI        KspI        MamI        MflI        MluI        
MluNI       MroI        MscI        MspA1I      MvaI        
MvnI        MwoI        NaeI        NarI        NciI        
NcoI        NgoMIV      NheI        NlaIV       NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RsrII       SacII       SalI        SapI        Sau96I      
SbfI        ScaI        ScrFI       SexAI       SfaNI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        TaqI        TfiI        Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AcsI          191  AAATTT               [GA]^AATT[CT]
AluI          174  AGCT                 AG^CT
AlwI          318  GATCC                GGATC(4/5)
ApoI          191  AAATTT               [GA]^AATT[CT]
AspHI         173  GAGCTC               G[AT]GC[AT]^C
BanII         173  GAGCTC               G[GA]GC[CT]^C
BbvI          160  GCAGC                GCAGC(8/12)
BclI          444  TGATCA               T^GATCA
BfaI          170  CTAG                 C^TAG
BmyI          173  GAGCTC               G[AGT]GC[ACT]^C
BpmI          215  CTCCAG               CTGGAG(16/14)
BsaWI         229  ACCGGA               [AT]^CCGG[AT]
BsiHKAI       173  GAGCTC               G[AT]GC[AT]^C
BsiYI         230  CCGGAGAAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          230  CCGGAGAAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         436  GAGAC                GTCTC(1/5)
BsmFI         149  GGGAC                GGGAC(10/14)
Bsp1286I      173  GAGCTC               G[AGT]GC[ACT]^C
BspHI         177  TCATGA               T^CATGA
BsrDI          76  GCAATG               GCAATG(2/0)
BstXI          65  CCACTGAGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI           68  CTGAG                C^T[ACGT]AG
FbaI          444  TGATCA               T^GATCA
Fnu4HI        160  GCAGC                GC^[ACGT]GC
HapII         230  CCGG                 C^CGG
HpaII         230  CCGG                 C^CGG
ItaI          160  GCAGC                GC^[ACGT]GC
MaeI          170  CTAG                 C^TAG
MaeII         401  ACGT                 A^CGT
MfeI           38  CAATTG               C^AATTG
MspI          230  CCGG                 C^CGG
MunI           38  CAATTG               C^AATTG
NdeI          155  CATATG               CA^TATG
NlaIII        178  CATG                 CATG^
RcaI          177  TCATGA               T^CATGA
SacI          173  GAGCTC               GAGCT^C
TseI          160  GCAGC                G^C[AT]GC
Tsp45I        103  GTCAC                ^GT[GC]AC
TspRI          66  CACTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

AfaI           62  GTAC                 GT^AC
              241  GTAC                
DpnII         318  GATC                 ^GATC
              445  GATC                
EarI           93  CTCTTC               CTCTTC(1/4)
              220  GAAGAG              
HphI          294  GGTGA                GGTGA(8/7)
              388  TCACC               
Ksp632I        93  CTCTTC               CTCTTC(1/4)
              220  GAAGAG              
MaeIII        103  GTCAC                ^GT[ACGT]AC
              113  GTTAC               
MboI          318  GATC                 ^GATC
              445  GATC                
MslI           66  CACTGAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              396  CACAGACGTG          
NdeII         318  GATC                 ^GATC
              445  GATC                
RsaI           62  GTAC                 GT^AC
              241  GTAC                
Sau3AI        318  GATC                 ^GATC
              445  GATC                


#########################################
# 3 OR MORE CUT ENZYMES (    5 enzymes) #
#########################################

MboII       MnlI        MseI        Tru9I       Tsp509I