##################################
# NO CUT ENZYMES (  206 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsiYI       BslI        BsmBI       BsmFI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstF5I      BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FauI        FokI        FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HindIII     HinfI       HpaII       
KasI        KpnI        Ksp632I     KspI        MaeI        
MaeII       MamI        MboII       MfeI        MflI        
MluI        MluNI       MroI        MscI        MslI        
MspI        MunI        MvnI        MwoI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        SexAI       SfaNI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TaqI        TfiI        Tsp509I     
TspRI       Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AfaI          108  GTAC                 GT^AC
AlwNI          14  CAGCAACTG            CAG[ACGT][ACGT][ACGT]^CTG
AvaII         138  GGACC                G^G[AT]CC
BanII         159  GAGCCC               G[GA]GC[CT]^C
BclI          224  TGATCA               T^GATCA
BmyI          159  GAGCCC               G[AGT]GC[ACT]^C
BsaJI         176  CCGAGG               C^C[ACGT][ACGT]GG
BseRI          28  CTCCTC               GAGGAG(10/8)
BsmAI          66  GAGAC                GTCTC(1/5)
Bsp1286I      159  GAGCCC               G[AGT]GC[ACT]^C
BsrI          246  ACTGG                ACTGG(1/-1)
BssKI          96  CCTGG                ^CC[ACGT]GG
BstNI          96  CCTGG                CC^[AT]GG
Cfr13I        138  GGACC                G^G[ACGT]CC
DdeI          104  CTCAG                C^T[ACGT]AG
DpnII         225  GATC                 ^GATC
DraII         137  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoO109I      137  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRII         96  CCTGG                ^CC[AT]GG
FbaI          224  TGATCA               T^GATCA
HincII        250  GTTAAC               GT[CT]^[GA]AC
HindII        250  GTTAAC               GT[CT]^[GA]AC
HpaI          250  GTTAAC               GTT^AAC
HphI           32  TCACC                GGTGA(8/7)
MaeIII        121  GTGAC                ^GT[ACGT]AC
MboI          225  GATC                 ^GATC
MseI          251  TTAA                 T^TAA
MspA1I        211  CAGCTG               C[AC]G^C[GT]G
MvaI           96  CCTGG                CC^[AT]GG
NdeII         225  GATC                 ^GATC
NlaIII         83  CATG                 CATG^
NlaIV          78  GGAGCC               GG[ACGT]^[ACGT]CC
PpuMI         137  AGGACCT              [GA]G^G[AT]CC[CT]
PstI           39  CTGCAG               CTGCA^G
PvuII         211  CAGCTG               CAG^CTG
RsaI          108  GTAC                 GT^AC
Sau3AI        225  GATC                 ^GATC
Sau96I        138  GGACC                G^G[ACGT]CC
ScaI          107  AGTACT               AGT^ACT
ScrFI          96  CCTGG                CC^[ACGT]GG
SfcI           39  CTGCAG               C^T[GA][CT]AG
SmlI          258  CTTGAG               C^T[CT][GA]AG
Tru9I         251  TTAA                 T^TAA
Tsp45I        121  GTGAC                ^GT[GC]AC


###################################
# TWO CUT ENZYMES (    3 enzymes) #
###################################

Cac8I           9  GCTCGC               GC[ACGT]^[ACGT]GC
              288  GCCTGC              
HaeIII        174  GGCC                 GG^CC
              180  GGCC                
MnlI           30  CCTC                 CCTC(7/6)
              178  GAGG                


#########################################
# 3 OR MORE CUT ENZYMES (    5 enzymes) #
#########################################

AluI        BbvI        Fnu4HI      ItaI        TseI