Restriction map for
pFN21AE3628
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# NO CUT ENZYMES ( 181 enzymes) #
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AatII Acc65I AccI AccII AccIII
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BcnI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
EcoT22I FauI FokI FseI FspI
HapII HgaI Hin1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MaeI MfeI MflI MluI
MluNI MroI MscI MseI MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tru9I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AclI 59 AACGTT AA^CGTT
AfaI 43 GTAC GT^AC
AlwI 127 GATCC GGATC(4/5)
AvaII 302 GGACC G^G[AT]CC
BclI 132 TGATCA T^GATCA
BpmI 201 CTCCAG CTGGAG(16/14)
BsaBI 127 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 79 GAGGAG GAGGAG(10/8)
BsgI 223 GTGCAG GTGCAG(16/14)
BsmAI 288 GAGAC GTCTC(1/5)
CfoI 153 GCGC GCG^C
Cfr13I 302 GGACC G^G[ACGT]CC
DraII 301 GGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 119 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 426 TGGCCG [CT]^GGCC[GA]
EarI 400 GAAGAG CTCTTC(1/4)
EcoO109I 301 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 352 GAATTC G^AATTC
EcoT14I 347 CCAAGG C^C[AT][AT]GG
FbaI 132 TGATCA T^GATCA
HaeII 152 AGCGCC [GA]GCGC^[CT]
HhaI 153 GCGC GCG^C
HinP1I 153 GCGC G^CGC
Ksp632I 400 GAAGAG CTCTTC(1/4)
MamI 127 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 436 CATGCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 51 CAGCTG C[AC]G^C[GT]G
MwoI 103 GCTTCCCAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 301 GGGACC GG[ACGT]^[ACGT]CC
PleI 199 GACTC GAGTC(4/5)
PpuMI 301 GGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 59 AACGTT AA^CGTT
PvuII 51 CAGCTG CAG^CTG
RsaI 43 GTAC GT^AC
Sau96I 302 GGACC G^G[ACGT]CC
SfcI 183 CTACAG C^T[GA][CT]AG
StyI 347 CCAAGG C^C[AT][AT]GG
TfiI 513 GATTC G^A[AT]TC
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AciI 429 CCGC CCGC(-3/-1)
488 CCGC
AcsI 280 GAATTT [GA]^AATT[CT]
352 GAATTC
ApoI 280 GAATTT [GA]^AATT[CT]
352 GAATTC
BbvI 50 GCAGC GCAGC(8/12)
343 GCTGC
BmyI 165 GGGCAC G[AGT]GC[ACT]^C
209 GTGCCC
BsiYI 156 CCATTCACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
396 CCCAGAAGAGG
BslI 156 CCATTCACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
396 CCCAGAAGAGG
BsmFI 301 GGGAC GGGAC(10/14)
494 GGGAC
Bsp1286I 165 GGGCAC G[AGT]GC[ACT]^C
209 GTGCCC
BsrDI 261 CATTGC GCAATG(2/0)
357 CATTGC
Cac8I 53 GCTGGC GC[ACGT]^[ACGT]GC
207 GCGTGC
DdeI 99 CTCAG C^T[ACGT]AG
121 CTCAG
HinfI 199 GACTC G^A[ACGT]TC
513 GATTC
HphI 369 GGTGA GGTGA(8/7)
173 TCACC
MaeII 60 ACGT A^CGT
270 ACGT
MaeIII 179 GTGAC ^GT[ACGT]AC
272 GTCAC
MboII 400 GAAGA GAAGA(8/7)
418 GAAGA
NlaIII 292 CATG CATG^
436 CATG
NspI 291 ACATGC [GA]CATG^[CT]
435 ACATGC
TseI 50 GCAGC G^C[AT]GC
343 GCTGC
Tsp45I 179 GTGAC ^GT[GC]AC
272 GTCAC
Tsp509I 281 AATT ^AATT
353 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
AluI BsaJI BsrI BssKI BstNI
DpnII EcoRII Fnu4HI HaeIII ItaI
MboI MnlI MvaI NdeII Sau3AI
ScrFI TspRI