Restriction map for
pFN21AE6136
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# NO CUT ENZYMES ( 198 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BalI BanI BbeI BbrPI
BbsI BcgI BclI BcnI BfaI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
Cac8I CelII Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HapII HgaI Hin1I HincII HindII
HindIII HpaI HpaII HphI KasI
KpnI KspI MaeI MaeII MamI
MfeI MluI MluNI MroI MscI
MslI MspA1I MspI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacII SalI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AspHI 199 GAGCTC G[AT]GC[AT]^C
BamHI 170 GGATCC G^GATCC
BanII 199 GAGCTC G[GA]GC[CT]^C
BbvCI 136 CCTCAGC CCTCAGC(-5/-2)
BbvI 268 GCTGC GCAGC(8/12)
BciVI 259 GGATAC GTATCC(6/5)
BglI 22 GCCATCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 199 GAGCTC G[AGT]GC[ACT]^C
BsiHKAI 199 GAGCTC G[AT]GC[AT]^C
Bsp1286I 199 GAGCTC G[AGT]GC[ACT]^C
BssKI 27 CCTGG ^CC[ACGT]GG
BssSI 196 CACGAG CACGAG(-5/-1)
BstF5I 24 CATCC GGATG(2/0)
BstNI 27 CCTGG CC^[AT]GG
BstYI 170 GGATCC [GA]^GATC[CT]
CfoI 294 GCGC GCG^C
Cfr13I 302 GGGCC G^G[ACGT]CC
DsaI 248 CCATGG C^C[GA][CT]GG
EaeI 29 TGGCCG [CT]^GGCC[GA]
EarI 3 GAAGAG CTCTTC(1/4)
EcoRII 27 CCTGG ^CC[AT]GG
EcoT14I 248 CCATGG C^C[AT][AT]GG
FokI 24 CATCC GGATG(9/13)
HaeII 293 AGCGCC [GA]GCGC^[CT]
HhaI 294 GCGC GCG^C
HinP1I 294 GCGC G^CGC
Ksp632I 3 GAAGAG CTCTTC(1/4)
MaeIII 46 GTAAC ^GT[ACGT]AC
MboII 3 GAAGA GAAGA(8/7)
MflI 170 GGATCC [GA]^GATC[CT]
MvaI 27 CCTGG CC^[AT]GG
NcoI 248 CCATGG C^CATGG
SacI 199 GAGCTC GAGCT^C
SapI 3 GAAGAGC GCTCTTC(1/4)
Sau96I 302 GGGCC G^G[ACGT]CC
ScrFI 27 CCTGG CC^[ACGT]GG
SfcI 225 CTACAG C^T[GA][CT]AG
StyI 248 CCATGG C^C[AT][AT]GG
TseI 268 GCTGC G^C[AT]GC
XhoII 170 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AciI 32 CCGC CCGC(-3/-1)
40 GCGG
AluI 153 AGCT AG^CT
200 AGCT
BsaJI 248 CCATGG C^C[ACGT][ACGT]GG
297 CCGAGG
DpnII 12 GATC ^GATC
171 GATC
Fnu4HI 31 GCCGC GC^[ACGT]GC
268 GCTGC
HaeIII 30 GGCC GG^CC
303 GGCC
HinfI 61 GAATC G^A[ACGT]TC
79 GAATC
ItaI 31 GCCGC GC^[ACGT]GC
268 GCTGC
MboI 12 GATC ^GATC
171 GATC
MseI 92 TTAA T^TAA
104 TTAA
MwoI 22 GCCATCCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
31 GCCGCCTTAGC
NdeII 12 GATC ^GATC
171 GATC
NlaIV 170 GGATCC GG[ACGT]^[ACGT]CC
301 GGGGCC
Sau3AI 12 GATC ^GATC
171 GATC
TfiI 61 GAATC G^A[AT]TC
79 GAATC
Tru9I 92 TTAA T^TAA
104 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 4 enzymes) #
#########################################
AlwI DdeI MnlI NlaIII