##################################
# NO CUT ENZYMES (  211 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        AluI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BciVI       
BclI        BcnI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrFI       BsrGI       BsrI        BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      ClaI        
CpoI        DdeI        DraI        DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT14I     FauI        
FbaI        FokI        FseI        FspI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
HpaII       HphI        KasI        KpnI        Ksp632I     
KspI        MaeI        MaeII       MamI        MfeI        
MluI        MluNI       MroI        MscI        MseI        
MspA1I      MspI        MunI        MvnI        MwoI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        TaqI        Tru9I       Tth111I     TthHB8I     
Van91I      XbaI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

AcsI          197  AAATTT               [GA]^AATT[CT]
AlwI          117  GGATC                GGATC(4/5)
ApoI          197  AAATTT               [GA]^AATT[CT]
AvaII         303  GGACC                G^G[AT]CC
BbvI           17  GCAGC                GCAGC(8/12)
BcgI          278  GCAAGTATATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaJI          12  CCTGGG               C^C[ACGT][ACGT]GG
BsiYI          76  CCTAATGAGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           76  CCTAATGAGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         172  GAGAC                GTCTC(1/5)
BsrDI          53  GCAATG               GCAATG(2/0)
BstYI         117  GGATCT               [GA]^GATC[CT]
Cfr13I        303  GGACC                G^G[ACGT]CC
DraII         302  AGGACCC              [GA]G^G[ACGT]CC[CT]
EcoO109I      302  AGGACCC              [GA]G^G[ACGT]CC[CT]
Fnu4HI         17  GCAGC                GC^[ACGT]GC
ItaI           17  GCAGC                GC^[ACGT]GC
MaeIII         43  GTGAC                ^GT[ACGT]AC
MflI          117  GGATCT               [GA]^GATC[CT]
MslI           36  CACCTTCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NlaIV         303  GGACCC               GG[ACGT]^[ACGT]CC
NspI          175  ACATGC               [GA]CATG^[CT]
PpuMI         302  AGGACCC              [GA]G^G[AT]CC[CT]
Sau96I        303  GGACC                G^G[ACGT]CC
SfaNI          58  GCATC                GCATC(5/9)
TseI           17  GCAGC                G^C[AT]GC
Tsp45I         43  GTGAC                ^GT[GC]AC
Tsp509I       198  AATT                 ^AATT
TspRI         327  CAGTG                CA[GC]TG[ACGT][ACGT]^
XcmI          104  CCAGGAAATGCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         117  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

DpnII          27  GATC                 ^GATC
              118  GATC                
EcoT22I        56  ATGCAT               ATGCA^T
              111  ATGCAT              
HinfI          97  GATTC                G^A[ACGT]TC
              257  GAATC               
MboI           27  GATC                 ^GATC
              118  GATC                
MboII         297  GAAGA                GAAGA(8/7)
              260  TCTTC               
MnlI           82  GAGG                 CCTC(7/6)
              271  GAGG                
NdeII          27  GATC                 ^GATC
              118  GATC                
NsiI           56  ATGCAT               ATGCA^T
              111  ATGCAT              
Sau3AI         27  GATC                 ^GATC
              118  GATC                
TfiI           97  GATTC                G^A[AT]TC
              257  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (    6 enzymes) #
#########################################

BssKI       BstNI       EcoRII      MvaI        NlaIII      
ScrFI